تأثیر یک جلسه تمرین مقاومتی بر بیان ژن myoD در عضلۀ کند و تندانقباض رت‌های نر نژاد ویستار

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار فیزیولوژی ورزش، گروه تربیت بدنی دانشکدۀ علوم انسانی، دانشگاه لرستان

2 دانشیار فیزیولوژی ورزش، گروه تربیت بدنی دانشکدۀ علوم انسانی دانشگاه تربیت مدرس

3 دانشیار گروه هماتولوژی، دانشکدۀ علوم پزشکی دانشگاه تربیت مدرس

4 دانشیار فیزیولوژی ورزش، دانشکدۀ تربیت بدنی دانشگاه خوارزمی

5 دانشجوی دکتری فیزیولوژی ورزشی دانشگاه شهید چمران

چکیده

سلول‌های ماهواره‌ای، سلول‌های جوانه‌ای عضلات اسکلتی محسوب می‌شوند که بر اثر آسیب عضلانی فعال وارد چرخۀ سلولی می‌شوند. از جمله نشانه‌های فعال‌سازی و تکثیر این سلول‌ها افزایش بیان ژن myoD است. تناقضاتی در پاسخ این ژن در تارهای کند و تند به تمرینات مقاومتی وجود دارد، بنابراین هدف این تحقیق ارزیابی اثر یک جلسه تمرین مقاومتی بر بیان ژن myoD در عضلۀ اسکلتی تند و کندانقباض رت‌های نر نژاد ویستار است.
بدین منظور پانزده رت از انستیتو پاستور تهیه شد و در شرایط طبیعی (دما، چرخۀ تاریکی و روشنایی و دسترسی آزاد به آب و غذا) نگهداری و به‌صورت تصادفی به گروه مقاومتی (ده سر) و کنترل (پنج سر) تقسیم شدند. گروه مقاومتی یک جلسه تمرین مقاومتی را اجرا کردند، سپس 3 و 6 ساعت پس از تمرین، بی‌هوش و تشریح شدند. پس از آن عضلات نعلی و عضلۀ بازکنندۀ دراز انگشتان (EDL) خارج و برای تعیین میزان بیان ژن myoD در آنها از روشReal time RT-PCR  استفاده شد. با استفاده از آزمون آماری t اطلاعات به‌دست‌آمده ارزیابی شد.
در پایان نتایج نشان داد تمرین مقاومتی بیان ژن myoD در عضلۀ EDL را 36/2 برابر (غیرمعنادار) افزایش داد و بیان ژن myoD عضلۀ نعلی تغییر معناداری نکرد. پس می‌توان گفت ژن myoD در عضلۀ تندانقباض نسبت به عضلۀ کندانقباض سریع‌تر و بیشتر تحت تأثیر تمرین مقاومتی قرار می‌گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of resistance exercise on myoD expression in slow and fast muscles of wistar rats

نویسندگان [English]

  • mohammad fathi 1
  • Reza Gharakhanlou 2
  • masoud solimani 3
  • hamid rajabi 4
  • raziyeh rezaei 5
1 Assistant Professor of Exercise Physiology, Physical Education Department, Humanities Faculty, Lorestan University, Khoramabad, Iran
2 Associate Professor of Exercise Physiology, Physical Education Department, Humanities Faculty, Tarbiat Modares University, Tehran, Iran
3 Associate Professor, Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4 Associate Professor of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Kharazmi University, Karaj, Iran
5 Ph.D. Student of Exercise Physiology, Shahid Chamran University, Ahvaz, Iran
چکیده [English]

Abstract
Background and Objective: satellite cells are muscle stem cells that enter cycle cells due to myotrauma. Among activation and proliferation indices of these cells is increase of myoD gene expression. But these are contradiction in response of this gene to resistance exercise in fast and slow fibers. Thus, the aim of this study was to evaluate the effect of a single bout of resistance exercise on myoD gene expression in fast and slow skeletal muscle in Wistar male rats.
Materials and Methods: 15 rats prepared from Pasteur Institute and housed under natural conditions (temperature, light/dark (12-h) cycle, with ad Libitum access to food and water). The rats randomly divided to two groups Resistance exercise (n=10) and control (n=5); the Resistance exercise group performed a session resistance exercise. three and six hours following, the rats were anaesthetized and killed, than to determine myoD gene expression rate the soleus and Extensor digitorum longus (EDL) muscles were removed, to determine of expression rate, the Quantitative Real time RT-PCR was used. Data were analyzed by t test.
Results: the Resistance exercise increased myoD gene expression 2.36 flod (no significantly) in EDL muscle, the SOL myoD gene expression no changed significantly.
Conclusion: myoD gene in fast-twitch muscle than in slow-twitch muscle is more and faster affected by resistance exercise.

کلیدواژه‌ها [English]

  • Resistance exercise
  • myoD
  • Extensor digitorum longus and Soleus muscle
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