نشریه علوم زیستی ورزشی

اثر 4 هفته تمرین هوازی شنا و عصارۀ آبی گزنه بر بیان ژن‌های PGC1α و UCP-1 و غلظت‌های سرمی آیریزین و FGF21 در رت‌های دیابتی چاق

نوع مقاله : مقاله پژوهشی

نویسندگان

عباس رنجبری 1
خالید محمدزاده سلامت 2
صمد اکبرزاده 3
غلامرضا خمیسی پور 4
بهروز نعیمی 5

1 گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، واحد سنندج، سنندج، ایران.

2 نویسنده مسئول، گروه تربیت بدنی و علوم ورزشی، دانشکدۀ علوم انسانی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران

3 گروه بیوشیمی بالینی، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران

4 گروه خون شناسی و بانک خون دانشکدة پیراپزشکی، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران.

5 گروه قارچ شناسی پزشکی دانشگاه علوم پزشکی بوشهر، بوشهر، ایران

https://doi.org/10.22059/jsb.2023.345715.1546
چکیده
مقدمه: فعالیت ورزشی و عصارۀ گیاه گزنه موجب افزایش بیان ژن‌های درگیر در تبدیل بافت چربی سفید به بافت چربی قهوه‌ای می‌شود، اما اثر ترکیبی آنها در این مورد مشخص نیست. هدف از پژوهش حاضر بررسی اثر عصارۀ گیاه گزنه و تمرین هوازی بر غلظت سرمی آیریزین، FGF21  و بیان ژن PGC1α UCP1, در رت‌های دیابتی بود.
روش پژوهش: در یک کارآزمایی تجربی 70 سر رت نر نژاد ویستار (میانگین وزن 52/18±35/227 گرم) تصادفی به هفت گروه شامل کنترل سالم، کنترل دیابتی، عصارۀ گزنه، مِتفورمین، تمرین، گزنه+تمرین و مِتفورمین+تمرین تقسیم شدند. گروه‌های تمرینی به مدت چهار هفته، هر هفته پنج روز، هرروز از ۱۵ دقیقه (در  هفتة اول) تا۵۰ دقیقه (در  هفتة چهارم) تمرین شنای اجباری انجام دادند. گروه‌های گزنه 25/1 و متفورمین 100 میلی‌گرم بر هر کیلوگرم از وزن بدن خود از این دو ماده را روزانه به‌صورت گاواژ دریافت کردند. پس از پایان مداخله  نمونة خون، بافت‌های عضلة پشت ران، پانکراس و چربی(احشایی) رت‌ها برای بررسی متغیرهای پژوهش گرفته شد. آزمون تحلیل واریانس یکطرفه و آزمون تعقیبی بنفرونی با سطح معناداری 05/0، به‌منظور بررسی تغییرات داده‌ها استفاده شد.
یافته‌ها: غلظت آیریزین گروه مِتفورمین+تمرین در مقایسه با گروه کنترل دیابتی به‌طور معناداری بیشتر بود (001/0=P). تفاوت معناداری نیز در میزان غلظت FGF21 سرم گروه‌ها مشاهده نشد. افزایش معنادار بیان ژن‌های PGC1α و UCP-1 در همۀ گروه‌های درمان نسبت به گروه کنترل سالم و کنترل دیابتی مشاهده شد (05/0>P).
نتیجه‌گیری: مصرف همزمان عصارۀ گزنه و تمرین شنا موجب کاهش گلوکز خون و همچنین فعال شدن بیان ژن‌های PGC-1α و UCP-1 می‌شود که در روند قهوه‌ای شدن بافت چربی نقش دارند.

کلیدواژه‌ها

تمرین هوازی
دیابت
آیریزین
PGC1α
UCP-1
عصارۀ گزنه
موضوعات

عنوان مقاله English

The Effect of 4 Weeks Aerobic Swimming Training and Aqueous Urtica Dioica Extract on PGC-1α and UCP-1 Gene Expression and Serum Concentrations of Irisin and FGF21 in Diabetic Rats

نویسندگان English

abas ranjbari 1
Khalid Mohamadzadeh Salamat 2
Samad Akbarzadeh 3
Gholamreza Khamisipour 4
Behrouz Naeimi 5
1 Department of Physical Education and Sport Sciences, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran
2 . Corresponding Author Department of Physical Education and Sports Sciences, Faculty of Humanities, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
3 Department of Biochemistry, Bushehr University of Medical Sciences, Bushehr, Iran.
4 Department of Laboratory Hematology and Blood Bank, Bushehr University of Medical Sciences, Bushehr, Iran.
5 Department of Medical Mycology, Bushehr University of Medical Sciences, Bushehr, Iran
چکیده English

Introduction: Sports activity and Urtica Dioica extract consumption increase the expression of genes involved in the conversion of white to brown adipose tissue, but their combined effect is not yet clear. This study aimed to investigate the effect of Urtica dioica extract consumption and aerobic training on serum Irisin and FGF21 concentration and PGC-1α and UCP1 gene expression in diabetic obese rats.
Methods: In an experimental design, Seventy male Wistar rats (mean weight: 227.35± 18.52 grams) were randomly divided into seven Healthy Control, Diabetic Control, Urtica Dioica, Metformin, Training, Urtica Dioica + Training, and Metformin + Training groups. The Training groups performed forced swimming training for four weeks, five days per week, and each day from 15 minutes (in the first week) to 50 minutes (in the fourth week). Each day, Urtica Dioica and Metformin groups received 1.25 and 100 mg/kg of these two substances, respectively via gavage. After the end of the intervention, rats’ blood samples, posterior thigh muscle tissues, pancreas, and fat (visceral) were taken to check the research variables. One-way analysis of variance and Bonferroni's post hoc test with a significance level of 0.05 was used to analyze the data.
Results: The serum Irisin concentration of the Metformin + exercise group was significantly higher than the Diabetic Control group (P<0.05). No significant difference was observed in the serum FGF21 concentration of all groups. A significant increase in PGC-1α and UCP-1 gene expression was observed in all treatment groups compared to Healthy Control and Diabetic Control groups (P<0.05).
Conclusion: The simultaneous consumption of Urtica Dioica extract and swimming training reduces blood glucose and also activates the PGC-1α and UCP-1 gene expression, which play a role in adipose tissue browning.

کلیدواژه‌ها English

Aerobic Training
Diabetes
Irisin
PGC-1α
UCP-1
Urtica Dioica extract
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  • تاریخ دریافت 21 تیر 1401
  • تاریخ بازنگری 28 بهمن 1401
  • تاریخ پذیرش 02 اسفند 1401

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