نوع مقاله : مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
نویسندگان
1 گروه تربیت بدنی و علوم ورزشی، دانشکدۀ علوم انسانی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران
2 نویسنده مسئول، گروه تربیت بدنی و علوم ورزشی، دانشکدۀ علوم انسانی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران
3 گروه تربیت بدنی و علوم ورزشی، دانشکدۀ علوم انسانی، دانشگاه آزاد اسلامی، واحد مرودشت، مرودشت، ایران
4 گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران
چکیده
مقدمه: بیماری آلزایمر موجب تحلیل هیپوکامپ و از دست رفتن حافظه و زوال عقل می‌شود. به هر حال اثر تمرینات ورزشی اختیاری و اجباری و مصرف ژل رویال بر این بیماری به‌روشنی مشخص نیست. در این پژوهش تأثیر هشت هفته تمرین استقامتی اختیاری و اجباری و مصرف ژل رویال (RJ) بر بیان ژن NF-κB و عوامل آنتی‌اکسیدانی موش‌های صحرایی آلزایمری‌شده با تری متیل تین (TMT) بررسی شد.
روش پژوهش: پس از القای آلزایمر با TMT، 60 سر موش صحرایی نر به‌صورت تصادفی به هشت گروه شامل کنترل آلزایمری‌شدۀ کشتار هفتۀ اول و آخر، تمرین اختیاری (VT)، تمرین اجباری شنا (ST)، شم، تمرین اختیاری + مصرف ژل رویال(VT+ RJ)، تمرین اجباری + مصرف ژل رویال (ST+ RJ) و مصرف ژل رویال (RJ) تقسیم شدند. 12 سر موش دیگر در گروه‌های کنترل سالم کشتار هفتۀ اول و آخر قرار گرفتند. گروه‌های تمرین VT و ST به مدت هشت هفته، سه جلسه در هفته و هر جلسه به مدت 60 دقیقه با استفاده از اصل اضافه‌بار به‌ترتیب در چرخ دوار و استخر ویژۀ جوندگان شنا قرار گرفتند. گروه‌های مصرف RJ به مدت هشت هفته 100mg/kg/day را به‌صورت صفاقی دریافت کردند. برای تجزیه‌وتحلیل آماری مقادیر مالئون دی آلدئید (MDA)، آنتی‌اکسیدانت تام (TAC)، میکروگلوبولین2بتا (B2m) و NF-κB از ANOVA یکطرفه استفاده شد.
یافته‌ها: بیان ژن TAC در بافت هیپوکامپ گروه ST وVT نسبت به گروه سالم معنا‌دار بود (02/0 (P=، اما بیان ژن MDA (165/0P=)، B2m )060/0(P= و NF-κB (069/0P=) معنا‌دار نبود.
نتیجه‌گیری: به‌طور کلی تمرینات استقامتی اختیاری و اجباری و مصرف ژل رویال بیان ژن آنتی‌اکسیدان‌ها را در بافت هیپوکامپ موش‌های آلزایمری‌شده با TMT بهبود می‌بخشند.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The Effect of Eight Weeks of Voluntary and Forced Endurance Training and Royal Jelly Consumption on NF-κB Gene Expression and Antioxidant Agents in Trimethyltin-Treated Alzheimer's Rats
نویسندگان [English]
- Awat Hasanloei 1
- Khalid Mohamadzadeh Salamat 2
- Seyed Ali Hosseini 3
- Samad Akbarzadeh 4
1 Department of Physical Education and Sports Sciences, Faculty of Humanities, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
2 . Corresponding Author Department of Physical Education and Sports Sciences, Faculty of Humanities, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
3 Department of Physical Education and Sport Sciences, Faculty of Humanities, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran.
4 Department of Clinical Biochemistry, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
چکیده [English]
Introduction: Alzheimer's disease causes hippocampal degeneration, memory loss, and dementia. However, the effect of voluntary and forced endurance training and royal jelly consumption on this disease is not clear.
Aim:In this study, the effect of eight weeks of voluntary and forced endurance training and consumption of Royal Jelly (RJ) on NF-κB gene expression and antioxidant factors were investigated in trimethyltin (TMT)-treated Alzheimer’s rats.
Methods: After induction of Alzheimer's disease with TMT, 60 male rats were randomly divided into eight groups, including: first and last week slaughtered Alzheimer's rats control, voluntary training (VT), forced swimming training (ST), sham, voluntary training + RJ consumption (VT + RJ), forced swimming training + RJ Consumption (ST + RJ), and Royal Jelly Consumption (RJ) groups. Also, 12 rats were placed equally in the first and last week slaughtered healthy control groups. The VT and ST groups were placed in a spinning wheel and a special swimming pool for rodents respectively, for eight weeks, and three 60-minute sessions per week using the overload principle. The RJ groups peritoneally received 100 mg/kg/day of royal jelly for eight weeks. One-way ANOVA was used to statistically analyze the values of Malondialdehyde (MDA), Total Antioxidant Capacity (TAC), Beta 2 Microglobulin (B2m), and NF-κB.
Results: TAC gene expression in the hippocampal tissue of ST and VT groups was significant compared with the healthy group (P = 0.02), but MDA gene expression (P=0.165), B2m (P=0.060), and NF-κB (P=0.069) were not significant.
Conclusion: In general, voluntary and forced endurance training and Royal Jelly consumption improve the antioxidant gene expression in the hippocampal tissue of TMT-treated Alzheimer's rats.
کلیدواژه‌ها [English]
- Alzheimer'
- s
- Antioxidant
- Endurance Training
- Royal Jelly
- NF-κB
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