The Effect of High Intensity Interval Training and Continuous Endurance Training on Gene Expression of mir-1 and IGF-1 in Cardiomyocyte of Diabetic Male Rats

Document Type : Research Paper


1 Assistant Professor of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran

2 Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

3 Associate Professor of Cellular and Molecular Research Center, Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

4 Research Associate Professor of Endocrinology and Metabolism, Diabetes Research Center, Endocrinology and Metabolism Research Center, University of Medical Sciences, Tehran, Iran

5 Researcher of Endocrinology and Metabolism, Endocrinology and Metabolism Research Center, University of Medical Sciences, Tehran, Iran


This study aimed to compare high intensity interval training and continuous endurance training on the gene expression of mir-1 and IGF-1 in cardiomyocytes of diabetic rats. After induction of diabetes, 21 male Wistar rats were randomly divided into three groups (each group 7 subjects): control, continuous endurance training, high intensity interval training). The training program included 5 days a week for 8 weeks. Each endurance session consisted of 30 minutes of running with the intensity of 60% VO2max. Each interval session consisted of 4 intervals, each interval 3 minutes, with the intensity of 90% VO2max and 1 minute of recovery with the intensity of 30% VO2max between each two intervals. Gene expression of IGF-1 and mir-1 of left ventricle tissue was assessed by qRT PCR. The results showed that both types of training significantly reduced the gene expression of mir-1 compared to the control group, but this decline was severer in the high intensity interval group than the endurance group (P≥0.05). Also, the gene expression of IGF-1 significantly increased in both training groups compared to the control group but this increase was severer in high intensity interval group than the endurance group (P≥0.05). It seems that high intensity interval training can be an effective intervention to reduce the complications of diabetic cardiomyopathy by repression of mir-1.


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