تأثیر یک دوره تمرین تناوبی شدید و استقامتی تداومی بر بیان ژن mir-1 و IGF 1 در کاردیومیوسیت رت‌های نر دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار فیزیولوژی ورزش، دانشکدۀ علوم ورزشی، دانشگاه الزهرا، تهران، ایران

2 . استاد فیزیولوژی ورزش، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

3 .دانشیار مرکز تحقیقات سلولی و ملکولی و گروه هماتولوژی، دانشکدۀ پیراپزشکی، دانشگاه علوم پزشکی ایران، تهران، ایران

4 دانشیار پژوهشی غدد درون ریز و متابولیسم، مرکز تحقیقات دیابت، پژوهشکدۀ علوم بالینی غدد و متابولیسم، دانشگاه علوم پزشکی تهران، تهران، ایران

5 پزشک، محقق مرکز تحقیقات غدد و متابولیسم، پژوهشکدۀ علوم بالینی غدد و متابولیسم، دانشگاه علوم پزشکی تهران، تهران، ایران

چکیده

هدف از مطالعۀ حاضر مقایسۀ یک دوره تمرین تناوبی شدید با استقامتی تداومی بر بیان ژن mir-1 و IGF 1 در کاردیومیوسیت رت‌های دیابتی بود. 21 سر رت نر نژاد ویستار، پس از القای دیابت به‌صورت تصادفی به سه گروه هفت‌تایی (کنترل، استقامتی تداومی و تناوبی شدید) تقسیم شدند، برنامۀ تمرینی 5 روز در هفته به مدت 8 هفته بود، هر جلسۀ تمرین استقامتی 30 دقیقه دویدن با شدت 60 درصد VO2max و هر جلسۀ گروه تناوبی چهار تناوب سه‌دقیقه‌ای با شدت 90 درصد VO2max و یک دقیقه ریکاوری با شدت 30 درصد VO2max بین هر تناوب بود. بیان ژن IGF-1 و mir-1 از بافت بطن چپ، به‌وسیلۀ روش qRT PCR  سنجیده شد. نتایج نشان داد هر دو نوع تمرین سبب کاهش معنادار بیان ژن mir-1 نسبت به گروه کنترل شده است ، اما این کاهش در گروه تناوبی شدید نسبت به گروه استقامتی بیشتر بود (05/0P≥)؛ همچنین بیان ژن IGF-1 در هر دو گروه تمرینی نسبت به گروه کنترل افزایش معناداری یافته بود که این افزایش در گروه تناوبی شدید نسبت به گروه استقامتی بیشتر بود (05/0P≥). به‌نظر می‌رسد تمرین تناوبی شدید با سرکوب mir-1 می‌تواند مداخلۀ مؤثری برای کاهش عوارض کاردیومیوپاتی دیابتی باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of High Intensity Interval Training and Continuous Endurance Training on Gene Expression of mir-1 and IGF-1 in Cardiomyocyte of Diabetic Male Rats

نویسندگان [English]

  • Maryam Delfan 1
  • Mohammad Reza Kordi 2
  • Ali Asghar Ravasi 2
  • Majid Safa 3
  • Ensieh Nasli Esfahani 4
  • Kamelia Rambod 5
1 Assistant Professor of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran
2 Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 Associate Professor of Cellular and Molecular Research Center, Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
4 Research Associate Professor of Endocrinology and Metabolism, Diabetes Research Center, Endocrinology and Metabolism Research Center, University of Medical Sciences, Tehran, Iran
5 Researcher of Endocrinology and Metabolism, Endocrinology and Metabolism Research Center, University of Medical Sciences, Tehran, Iran
چکیده [English]

This study aimed to compare high intensity interval training and continuous endurance training on the gene expression of mir-1 and IGF-1 in cardiomyocytes of diabetic rats. After induction of diabetes, 21 male Wistar rats were randomly divided into three groups (each group 7 subjects): control, continuous endurance training, high intensity interval training). The training program included 5 days a week for 8 weeks. Each endurance session consisted of 30 minutes of running with the intensity of 60% VO2max. Each interval session consisted of 4 intervals, each interval 3 minutes, with the intensity of 90% VO2max and 1 minute of recovery with the intensity of 30% VO2max between each two intervals. Gene expression of IGF-1 and mir-1 of left ventricle tissue was assessed by qRT PCR. The results showed that both types of training significantly reduced the gene expression of mir-1 compared to the control group, but this decline was severer in the high intensity interval group than the endurance group (P≥0.05). Also, the gene expression of IGF-1 significantly increased in both training groups compared to the control group but this increase was severer in high intensity interval group than the endurance group (P≥0.05). It seems that high intensity interval training can be an effective intervention to reduce the complications of diabetic cardiomyopathy by repression of mir-1.

کلیدواژه‌ها [English]

  • Cardiomyopathy
  • IGF-1
  • microRNA-1
  • Training
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