Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


1 MSc, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran

2 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran

3 Professor, Department of Immunogenetics, Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran

4 Assistant Professor, Department of Physical Education, Faculty of Humanities, University of Ayatollah Alozma Boroujerdi, Boroujerd, Lorestan, Iran


Previous studies have investigated the effect of continuous aerobic training (CAT) on gene expression of positive regulatory domain-containing protein 16 (PRDM16) in white adipose tissue. However, no study has yet investigated the effect of different models of training especially high intensity interval training (HIIT) on the expression of this gene. Thus, the aim of this study was to investigate the effect of two models of HIIT and two models of CAT on gene expression of PRDM16. 40 rats were divided randomly into five groups: 1) control 2) moderate volume CAT 3) high volume CAT 4) moderate volume HIIT and 5) high volume HIIT. The subjects of training groups underwent two models of HIIT and two models of CAT on a treadmill for 8 weeks, 5 sessions per week. After the last training session, rats were euthanized and subcutaneous adipose tissue were dissected. The Real Time–PCR method was used to measure the relative gene expression of PRDM16. Data showed that the gene expression of PRDM16 had no significant difference in two HIIT and two CAT groups compared with the control group (P>0.05). The results of this study indicated that training exercises including CAT and HIIT do not change the gene expression of PRDM16 in subcutaneous adipose tissue.


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