Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


1 PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran

2 . Professor, PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran

3 Associate Professor, Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran


The vanilloid receptor 1 (TRPV1) is a nociceptor receptor in sensitive system which is activated by capsaicin, heat, acidity and some metabolites. The role of this cation receptor is known at pain. New reports suggest that TRPV1 plays a role in other processes and researchers are studying the effect of this receptor on exercise pressure reflex (EPR) and cardiovascular response in some diseases.  In this experimental study, the aim of this study was to find an appropriate answer to perceive the effect of moderate continuous training on the expression of TRPV1 protein in soleus muscle. 16 male Wistar rats (weight: 200-220 gr) were randomly assigned to 2 groups: training and control. The training group performed 6 weeks (5 sessions per week) of moderate continuous training. The control group did not have any training but there was no difference between these groups in all conditions. The rats were anesthetized, sacrificed and their required tissues were extracted by standard procedures. The results of independent t test derived from Western Blotting laboratory findings for TRPV1 receptor protein expression showed a significant increase (P=0.033) in the expression of this receptor in soleus muscle in the training group compared with the control group. These results show that sensory nerves are adapted to training with an increase in TRPV1 receptor protein expression.


1.   Nilius, B., TRP channels in disease. Biochim Biophys Acta, 2007. 1772(8): p. 805-12.
2.   Caterina, M.J., Vanilloid receptors take a TRP beyond the sensory afferent. Pain, 2003. 105(1-2): p. 5-9.
3.   Xin, H.T., H. Yamaguchi, M. Takemori, S. Nakamura, A. Kohama, K., Vanilloid receptor expressed in the sarcoplasmic reticulum of rat skeletal muscle. Biochem Biophys Res Commun, 2005. 332(3): p. 756-62.
4.   Caterina, M.J.L., A. Malmberg, A. B. Martin, W. J.Trafton, Petersen-Zeitz, K. R. Koltzenburg, M., A.I. Basbaum, and D. Julius, Impaired nociception and pain sensation in mice lacking the capsaicin receptor. Science, 2000. 288(5464): p. 306-13.
5.   Kauer, J.A.G., H. E., Hot flash: TRPV channels in the brain. Trends Neurosci, 2009. 32(4): p. 215-24.
6.   Moriyama, T.H., T. Togashi, K. Iida, T. Segi, E. Sugimoto, Y., et al., Sensitization of TRPV1 by EP1 and IP reveals peripheral nociceptive mechanism of prostaglandins. Mol Pain, 2005. 1: p. 3.
7.   White, J.P.C., M. Rei Fidalgo, A. Paule, C. C. Noormohamed, F. Urban, L., M. Maze, and I. Nagy, Role of transient receptor potential and acid-sensing ion channels in peripheral inflammatory pain. Anesthesiology, 2010. 112(3): p. 729-41.
8.   White, J.P.U., L. Nagy, I., TRPV1 function in health and disease. Curr Pharm Biotechnol, 2011. 12(1): p. 130-44.
9.   Mizuno, M.M., M. N.Mitchell, J. H. Smith, S. A., Antagonism of the TRPv1 receptor partially corrects muscle metaboreflex overactivity in spontaneously hypertensive rats. J Physiol, 2011. 589(Pt 24): p. 6191-204.
10. Spicarova, D.N., V. Palecek, J., Update on the role of spinal cord TRPV1 receptors in pain modulation. Physiol Res, 2014. 63 Suppl 1: p. S225-36.
11. Samuel P. Wanner, A.G., and Andrej A, Hyperactive when young, hypoactive and overweight when aged: Connecting the dots in the story about locomotor activity, body mass, and aging in Trpv1 knockout mice. BMC Complement Altern Med, 2011. 3: p. 4.
12. Yang, D.L., Z.Ma, S.Wong, W. T.Ma, L. Zhong, J., et al., Activation of TRPV1 by dietary capsaicin improves endothelium-dependent vasorelaxation and prevents hypertension. Cell Metab, 2010. 12(2): p. 130-41.
13. Notay, K.K., S. L. Lee, J. B. Doherty, C. J. Seed, J. D. Swiatczak, M., D.M. Mutch, and P.J. Millar, TRPV1 and BDKRB2 receptor polymorphisms can influence the exercise pressor reflex. J Physiol, 2018. 596(21): p. 5135-5148.
14. Snitker, S.F., Y. Shen, H. Ott, S. Pi-Sunyer, X. Furuhata, Y. Sato, H. and M. Takahashi, Effects of novel capsinoid treatment on fatness and energy metabolism in humans: possible pharmacogenetic implications. Am J Clin Nutr, 2009. 89(1): p. 45-50.
15. Kim, K.M.K., T. Ishihara, K. Inoue, K. Fushiki, T., Increase in swimming endurance capacity of mice by capsaicin-induced adrenal catecholamine secretion. Biosci Biotechnol Biochem, 1997. 61(10): p. 1718-23.
16. Shin, K.O.M., T., Alterations of autonomic nervous activity and energy metabolism by capsaicin ingestion during aerobic exercise in healthy men. J Nutr Sci Vitaminol (Tokyo), 2007. 53(2): p. 124-32.
17. Lee, Y.M., K. Talbert, E. E. Kavazis, A. N. Smuder, A. J. Willis, W. T. and S.K. Powers, Exercise protects cardiac mitochondria against ischemia-reperfusion injury. Med Sci Sports Exerc, 2012. 44(3): p. 397-405.
18. Smith, S.A.M., J. H. Li, J., Independent modification of baroreceptor and exercise pressor reflex function by nitric oxide in nucleus tractus solitarius. Am J Physiol Heart Circ Physiol, 2005. 288(5): p. H2068-76.
19. Luo, Z.M., L. Zhao, Z. He, H. Yang, D. Feng, X., et al., TRPV1 activation improves exercise endurance and energy metabolism through PGC-1alpha upregulation in mice. Cell Res, 2012. 22(3): p. 551-64.
20. Amaya, F.S., G. Nagano, M. Ueda, M. Hashimoto, S. Tanaka, Y., H. Suzuki, and M. Tanaka, NGF and GDNF differentially regulate TRPV1 expression that contributes to development of inflammatory thermal hyperalgesia. Eur J Neurosci, 2004. 20(9): p. 2303-10.
21. Chuang, H.H.P., E. D. Kong, H. Shields, S. Jordt, S. E. Basbaum, A. I., M.V. Chao, and D. Julius, Bradykinin and nerve growth factor release the capsaicin receptor from PtdIns(4,5)P2-mediated inhibition. Nature, 2001. 411(6840): p. 957-62.
22. Zhang, L.L.Y.L., D.Ma,L. Q. Luo, Z. D. Cao, T. B. Zhong, J., et al., Activation of transient receptor potential vanilloid type-1 channel prevents adipogenesis and obesity. Circ Res, 2007. 100(7): p. 1063-70.
23. Oh, T.W.O., T. W. Ohta, F., Dose-dependent effect of capsaicin on endurance capacity in rats. Br J Nutr, 2003. 90(3): p. 515-20.
24. Dousset, E.M., T. Decherchi, P. Jammes, Y. Grelot, L., Effects of neonatal capsaicin deafferentation on neuromuscular adjustments, performance, and afferent activities from adult tibialis anterior muscle during exercise. J Neurosci Res, 2004. 76(5): p. 734-41.
25. Trudeau, F.M., M., Capsaicin-sensitive nerves and endurance exercise in the rat. Physiol Behav, 1996. 59(2): p. 355-9.
26. Rong, W.H., K. Davis, J. B. Hicks, G. Winchester, W. J. Grundy, D., Jejunal afferent nerve sensitivity in wild-type and TRPV1 knockout mice. J Physiol, 2004. 560(Pt 3): p. 867-81.
27. Wang, X.M., R. L. Ahern, G. P., Oleoylethanolamide excites vagal sensory neurones, induces visceral pain and reduces short-term food intake in mice via capsaicin receptor TRPV1. J Physiol, 2005. 564(Pt 2): p. 541-7.
28. Guerfali, I.M., C. Durieux, A. C. Bonnefoy, R. Bartegi, A. Freyssenet, D., Calcineurin A and CaMKIV transactivate PGC-1alpha promoter, but differentially regulate cytochrome c promoter in rat skeletal muscle. Pflugers Arch, 2007. 454(2): p. 297-305.
29. Xue, Q.J., B. Chen, T. Schumacher, M. A., Transcription of rat TRPV1 utilizes a dual promoter system that is positively regulated by nerve growth factor. J Neurochem, 2007. 101(1): p. 212-22.
30. Aloe, L.R., M. L. Bianchi, P. Manni, L., Nerve growth factor: from the early discoveries to the potential clinical use. J Transl Med, 2012. 10: p. 239.
31. Smith, S.A.M., J. H. Garry, M. G., The mammalian exercise pressor reflex in health and disease. Exp Physiol, 2006. 91(1): p. 89-102.
32. Chae, C.H.J., S. L. An, S. H. Jung, C. K. Nam, S. N. Kim, H. T., Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats. J Physiol Biochem, 2011. 67(2): p. 235-41.
33. Garry, M.G., Abnormalities of the exercise pressor reflex in heart failure. Exerc Sport Sci Rev, 2011. 39(4): p. 167-76.
34. Wang, H.J.L., Y. L. Gao, L. Zucker, I. H. Wang, W., Alteration in skeletal muscle afferents in rats with chronic heart failure. J Physiol, 2010. 588(Pt 24): p. 5033-47.
35. Ota, H.K., K. Murase, S. Kashio, M. Tominaga, M. and K. Mizumura, TRPV1 and TRPV4 play pivotal roles in delayed onset muscle soreness. PLoS One, 2013. 8(6): p. e65751.
36. Smith, S.A.L., A. K. Williams, M. A. Murphy, M. N.Mitchell, J. H.Garry, M. G., The TRPv1 receptor is a mediator of the exercise pressor reflex in rats. J Physiol, 2010. 588(Pt 7): p. 1179-89.