The Effect of 4 Weeks of High Intensity Interval Training on Pro-Angiogenesis Gene Expression of Endothelial Cells in Skeletal Muscle of Rats

Document Type : Research Paper I Open Access I Released under CC BY 4.0 license


1 PhD Student of Health in Disaster and Emergency Situations, Kerman University of Medical Sciences, Kerman, Iran

2 MSc in Exercise Physiology, University of Tehran, Tehran, Iran

3 Associate Professor, Department of Exercise Physiology, University of Tehran, Tehran, Iran

4 . MSc in injuries and corrective exercises, University of Tehran, Tehran, Iran


Mir-210 is pro-angiogenic micro-RNA in endothelial cells that improves angiogenesis process by suppressing (EphrinA3) and increasing the migration of some gene and protein targets (VEGF). In this study, 12 male rats (age: 8 weeks, mean weight: 180±20 g) were selected and randomly divided into control (n=6) and exercise (n=6) groups. High intensity interval training was performed for 4 weeks, 5 days a week including 3 high intervals (4 minutes at 90-100% of VO2max) and 3 low intervals (2 minutes at 50-60% VO2max). Gene expression was calculated by Real time-PCR technique and 2-∆∆CT. Independent t test was used to determine the significance of variables between the groups.The results showed that high intensity interval training significantly changed the increase of gene expression of Mir-210 and VEGF (P=0.005) (P=0.003). Also, the decrease in gene expression of EphrinA3 receptor in the exercise group was significant compared with the control group (P=0.000). Generally, perhaps due to hypoxiawhich happened along with high intensity interval training, increased gene expression of Mir-210 and VEGF improved the pro-angiogenic function of endothelial cells and this adaptation increased angiogenesis in rats.


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Volume 9, Issue 3
November 2017
Pages 443-456
  • Receive Date: 22 January 2017
  • Revise Date: 31 May 2017
  • Accept Date: 12 July 2017
  • First Publish Date: 22 November 2017