TY - JOUR ID - 64791 TI - The Effect of 4 Weeks of High Intensity Interval Training on Pro-Angiogenesis Gene Expression of Endothelial Cells in Skeletal Muscle of Rats JO - Journal of Sport Biosciences JA - JSB LA - en SN - 2008-9325 AU - Aminizadeh, Mohsen AU - Shafiee, Ahad AU - Kordi, Mohammas Reza AU - Nabavi Zadeh, Mahla sadat AD - PhD Student of Health in Disaster and Emergency Situations, Kerman University of Medical Sciences, Kerman, Iran AD - MSc in Exercise Physiology, University of Tehran, Tehran, Iran AD - Associate Professor, Department of Exercise Physiology, University of Tehran, Tehran, Iran AD - . MSc in injuries and corrective exercises, University of Tehran, Tehran, Iran Y1 - 2017 PY - 2017 VL - 9 IS - 3 SP - 443 EP - 456 KW - angiogenesis KW - high intensity interval training KW - MicroRNA KW - vascular endothelial growth factor DO - 10.22059/jsb.2018.64791 N2 - Mir-210 is pro-angiogenic micro-RNA in endothelial cells that improves angiogenesis process by suppressing (EphrinA3) and increasing the migration of some gene and protein targets (VEGF). In this study, 12 male rats (age: 8 weeks, mean weight: 180±20 g) were selected and randomly divided into control (n=6) and exercise (n=6) groups. High intensity interval training was performed for 4 weeks, 5 days a week including 3 high intervals (4 minutes at 90-100% of VO2max) and 3 low intervals (2 minutes at 50-60% VO2max). Gene expression was calculated by Real time-PCR technique and 2-∆∆CT. Independent t test was used to determine the significance of variables between the groups.The results showed that high intensity interval training significantly changed the increase of gene expression of Mir-210 and VEGF (P=0.005) (P=0.003). Also, the decrease in gene expression of EphrinA3 receptor in the exercise group was significant compared with the control group (P=0.000). Generally, perhaps due to hypoxiawhich happened along with high intensity interval training, increased gene expression of Mir-210 and VEGF improved the pro-angiogenic function of endothelial cells and this adaptation increased angiogenesis in rats. UR - https://jsb.ut.ac.ir/article_64791.html L1 - https://jsb.ut.ac.ir/article_64791_1f32d8533f510de96965995a530f765c.pdf ER -