The Effect of 8 Weeks of Aerobic Exercises with Ergometer and Nettle Extract Supplementation on Plasma Levels of Nesfatin-1 and C-Reactive Protein in Overweight and Obese Women

Document Type : Research Paper

Authors

1 MSc Student of Exercise Physiology, University of Sistan and Baluchestan, Zahedan, Iran

2 Assistant Professor of Exercise Physiology, Department of Physical Education and Sport Sciences, University of Sistan and Baluchestan, Zahedan, Iran

3 Associate Professor of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran

4 Associate Professor, Department of Nutritional Sciences and Industries, Zahedan University of Medical Sciences, Zahedan, Iran

Abstract

 
The aim of this study was to investigate the effect of 8 weeks of aerobic exercise with an ergometer and nettle extract supplementation on plasma levels of nesfatin-1 and C-reactive protein in overweight and obese women. A total of 46 overweight and obese women (age range of 25-45 years, BMI>25 kg/m2) were purposively selected and randomly divided into 4 groups: exercise+nettle (n=12), exercise+placebo (n=12), nettle (n=11), placebo (n=11). The nettle and placebo groups daily received 8 ml of nettle extraction and placebo solution respectively after their mail meal for 8 weeks. Aerobic exercises were conducted for 8 weeks, 3 sessions per week with an intensity of 60-75% of reserved heart rate. Blood samples were collected after 12 hours of fasting and also 48 hours after the exercises in experimental conditions. Nesfatin-1 plasma level significantly increased in exercise+nettle, exercise+placebo and nettle groups after 8 weeks of aerobic exercises and nettle supplementation (P<0.05) and exercise+nettle group had the highest increase. Weight changes, BMI and BF% showed a significant difference in all intervention groups compared to the pretest (P<0.01). Also, plasma level of CRP significantly reduced in exercise+nettle, exercise+placebo and nettle groups (P<0.05). In general, it seems that aerobic exercise on an ergometer as an effective training method with nettle extract supplementation can immunize the body against inflammatory diseases related to obesity through a reduction in CRP inflammatory marker and an increase in plasma levels of nesfatin-1.

Keywords


1. بشیری، جبار؛ غلامی، فرهاد؛ رهبران، عادل؛ ظرماهی، وحید (1391). «تأثیر یک جلسه فعالیت هوازی بر سطوح نسفاتین-1 سرمی مردان سالمند غیرورزشکار»، مجلۀ پزشکی دانشگاه علوم پزشکی تبریز، 34(4)، ص 30-25.
2. تاجی طبس، آسیه؛ مقرنسی، مهدی (1393). «اثر ده هفته تمرین مقاومتی بر سطوح سرمی نسفاتین-1 و شاخص مقاومت به انسولین در زنان مبتلا به دیابت نوع 2»، مجلۀ دیابت و متابولیسم ایران، 14(3)، ص 188-179.
3. توسلی، حسن؛ توفیقی، اصغر؛ حسین‌پناه، فرهاد؛ هدایتی، مهدی (1392). «اشتها و تمرین؛ اثر دوازده هفته تمرین مقاومتی دایره‌ای بر نسبت سطح نسفاتین-1 به گرلین آسیل دار در نوجوانان دارای اضافه‌وزن»، مجلۀ غدد درون‌ریز و متابولیسم ایران، 15(6)، ص 526-519.
4. ﺗﻮﻓﯿﻘﯽ، اﺻﻐﺮ؛ ﻣﻬﺮﺑﺎﻧﯽ، ﺟﻮاد؛ ﺧﺪﯾﻮی، ﺳﯿﺪ ﻣﺤﺴﻦ (1391). «اﺛﺮ ﻫﺸﺖ ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ اﺳﺘﻘﺎﻣﺖ ﻫﻮازی ﺑﺮ ﺗﻐﯿﯿﺮات ﻧﺴﻔﺎﺗﯿﻦ-1 و ﮔﺮﻟﯿﻦ آﺳﯿﻞ دار در ﻣﺮدان ﭼﺎق ﺟﻮان». ﻣﺠﻠۀ داﻧﺸﮑﺪۀ ﭘﺰﺷﮑﯽ داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰشکی ﻣﺸﻬﺪ؛ دورۀ 57 ﺷ 3، ص 570-562.
5. جعفری، مهری؛ مقرنسی، مهدی؛ سلیمی خراشاد، علیرضا (1394). «تأثیر هشت هفته تمرین استقامتی و مقاومتی دایره‌ای بر سطوح پلاسمایی کمرین و نسفاتین-1 دختران دارای اضافه‌وزن و چاق».[MO1] 
6. حسنی، علی؛ ابراهیمی، مریم؛ رمضانپور، محمدرضا (1394). «ﺑﺮرﺳﻲ تأثیر ﻫﺸﺖ ﻫﻔﺘﻪ ﺗﻤﺮﻳﻦ ﻣﻨﻈﻢ ﻫﻮازی ﺑﺮ ﻣﺼﺮف ﻋﺼﺎرۀ ﮔﺰﻧﻪ بر سطح ﮔﻠﻮﻛﺰ و ﺷﺎﺧﺺ ﻣﻘﺎوﻣﺖ ﺑﻪ اﻧﺴﻮﻟﻴﻦ زﻧﺎن دﻳﺎﺑﺘﻲ ﻧﻮع دو»، ﻣﺠﻠۀ داﻧﺶ و ﺗﻨﺪرﺳﺘﻲ، دورۀ 10، ﺷ 4.
7. حسینی کاخک، علیرضا؛ امیری پارسا، طیبه؛ آذرنیوه، م.، حامدی‌نیا، محمدرضا (1390).«تأثیر تمرینات هوازی، مقاومتی و بی تمرینی بر نیم‌رخ لیپیدی و CRP در دختران چاق»، مجلۀ دانشگاه علوم پزشکی و خدمات بهداشتی درمانی سبزوار، دورۀ18، ش3.
8. حق‌شناس، روح‌اﷲ؛ رواﺳﯽ، ﻋﻠﯽ‌اﺻﻐﺮ؛ ﮐﺮدی، ﻣﺤﻤﺪرﺿﺎ؛ ﻫﺪاﯾﺘﯽ، ﻣﻬﺪی؛ ﺷﺐ‌ﺧﯿﺰ، ﻓﺎﻃﻤﻪ؛ ﺷﺮﯾﻌﺖ‌زاده، ﻣﺤﻤﺪ (1390). «ﺗﺄﺛﯿﺮ دوازده ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ اﺳﺘﻘﺎﻣﺘﯽ ﺑﺮ وزن، درﯾﺎﻓﺖ ﻏﺬا و ﻧﺴﻔﺎﺗﯿﻦ- 1 ﭘﻼﺳﻤﺎی رتﻫﺎی ﻧﺮ ﭼﺎق». ورزش و ﻋﻠﻮم زﯾﺴﺖ ﺣﺮﮐﺘﯽ، دورۀ 3 ، ش 1، ص 85-77 .
9. حق‌شناس، روح‌الله؛ رواسی، علی‌اکبر؛ کردی، محمدرضا؛ هدایتی، مهدی؛ شب‌خیز، فاطمه؛ شریعت‌زاده جنیدی،محمد (1392). «تأثیر دوازده هفته تمرین استقامتی بر IL-6، IL-10 و نسفاتین-1 پلاسمای رت‌های نر چاق»، علوم زیستی ورزشی، 5(4): ص 122-109.
10. قنبرزاده، مختار؛ کاظمی، عبدالرضا (1394). «مقایسۀ سه شیوۀ مختلف تمرین هوازی بر سطوح پلاسمایی کمرین، مقاومت به انسولین و عملکرد جسمانی زنان سالمند»، مجلۀ دانش و تندرستی، 10(4)، ص 47-40.
11. ﻧﻤﺎزی، نازلی؛ ﻃﺮﻳﻘﺖ اسفنجانی، علی؛ آوری، ﻣسعود؛ حشمتی، جواد (1390). «اﺛﺮ ﻋﺼﺎرۀ ﺷﺎﺧﺺ ﻫﻴﺪرواﻟﻜﻠﻲ ﮔﺰﻧﻪ ﺑﺮ ﺣﺴﺎﺳﻴﺖ ﺑﻪ اﻧﺴﻮﻟﻴﻦ و ﺑﺮﺧﻲ از شاخص‌های اﻟﺘﻬﺎﺑﻲ در ﺑﻴﻤﺎران مبتلا به دﻳﺎﺑﺖ ﻧﻮع2»، ﻣﺠﻠۀ ﻋﻠﻤﻲ داﻧﺸﮕﺎه علوم پزشکی و خدمات بهداشتی درمانی همدان، دورۀ ﻫﺠدهم، ش 4.
12. وکیلی، جواد؛ حسین‌پور، لیلا (1394). « تأثیر هشت هفته تمرین هوازی و مصرف چای سبز بر برخی عوامل خطرزای قلبی- عروقی زنان چاق»، نشریۀ مطالعات کاربردی علوم زیستی در ورزش، دورۀ 3، ش 5.
13. Abedi B, (2012). »The effects of 12-wk combined aerobic/resistance training on C-reactive protein (CRP) serum and interleukin-6 (IL-6) plasma in sedentary men«. Yafteh.; 14: 95-106.
14. Abramson JL and Vaccarino V, (2002). »RelationshipBetween Physical Activity and Inflammation Among Apparently Hearty Middele-Aged and Other Us Adult«. Arch Intern Med .162:1286-1292.
15. Ahangarpour A, Mohammadian M, Dianat M.,( 2012). »Antidiabetic effect of hydroalcholic urtica dioica leaf extract in Male Rats with Fructose- Induced Insulin Resistance«. Iran J Med Sci; 37(3): 181-6.
16. Andreas O, Anke T, Nora K, Mathias F (2006). »Effect of a 4 week physical training program on plasma concentrations of inflammatory markers in patients with abnormal glucose tolerance«. Eur J Endocrinol;154: 577-85.
17. Bastard JP, Jardel C, Bruckert E, Blondy P, Capeau, Laville M, et al, (2000). »Elevated levels of interleukin-6 are reduced in serum and subcutaneous adipose tissue of obese women after weight loss«. J Clin Endocrinol Metab; 85: 3338-42.
18. Bluher M, (2012). »Clinical relevance of adipokines, Diabetes and Metabolism Journal«, Vol. 36, No. 5, pp. 317-327.
19. Chaolu H, Asakawa A, Ushikai M, Li YX, Cheng KC, Li JB, et al., (2011.) »Effect of exercise and high-fat diet on plasma adiponectin and nesfatin levels in mice«. Exp Ther Med; 2: 369-373.
20. Chrubasik JE, Roufogalis BD, Wagner H, Chrubasik S., (2007). »A comprehensive review on the stinging nettle effect and efficacy profiles«. Part II: urticae radix. Phytomedicine; 14: 568-79.
21. Dowling EA. (2001). »How exercise affects lipid profiles in women: what to recommend for patients«. Phys Sportsmed; 29(9): 45-52.
22. Foldes, G., Horkay, F., Szokodi, I., Vuolteenaho, O., Ilves, M., Lindstedt, KA., et al. (2003). »Circulating and Cardiac Levels of Apelin, the Novel Ligand of the Orphan Receptor APJ, in Patients with Heart Failure«. Biochem Biophys Res Commun., Vol. 308, No. 3, PP: 480-5.
23. Ghanbari-Niaki, A., Kraemer, R.R., Soltani, R., (2010). »Plasma nesfatin-1 and glucoregulatory hormone responses to two different anaerobic exercise sessions«, Eur J Appl Physiol. Vol. 110, No.4,pp.863-8.
24. Ghanbari-Niaki, A., Rahmati-Ahmadabad, S., Ansari-Pirsaraei, Z., (2013). »Effect of Aerobic Training on Tissu Nesfatin-1/Necleobindin-2 mRNA. Plasma Nesfatin-1 and High-density Lipoprotein Concentration in Female Rats«. Iranian Journal of Health and Physical Activity, Vol. 4,pp. 1-7.
25. Goebel-Stengel, M., Wang, L., (2013). »Central and peripheral expression and distribution of NUCB2/nesfatin-1«, Curr Pharm Des, Vol. 19, No.39, pp. 6935-40.
26. Hallschmid M, Patel S, et al., (2010). »Identification of nesfatin-1 in human and murine adipose tissue: a novel depot-specific adipokine with increased levels in obesity«. Endocrinology; 151: 3169-3180.
27. Hamedinia MR, Haghighi AH, Ravasi A., (2007). »The effect of aerobic training on inflammatory factors. of heart disease in obese men«. Iranian Journal of Harkat; 34: 47-58.
28. Katrhryn E (2005). »Inflammation,stress and diabetes«. J.Clin Invest; 1 (15): 1111-1119.
29. Kelly AS et al., (2007); »In the absence of weight loss, exercise training does not improve adipokines or oxidative stress in overweight children«. Metabolism Clinical Experimental.; 56: 1005-1009.
30. Kim K, Valentine RJ, Shin Y, Gong K., (2008). »Associations of visceral adiposity and exercise participation with C- reactive protein, insulin resistance, and endothelial dysfunction in Korean healthy adults«. Metabolism; 57: 1181-9.
31. Lakka T. A, Lakka H. M, Rankinen T, & et al. (2005). »Effect of exercise training on plasma levels of c-reactive protein in healthy adults: the heritage family study«. Eur Heart J, 26: 2018–25.
32. Liesen H, Dufaux B, Hollmann W., (1977). »Modifications of serum glycoproteins the days following a prolonged physical exercise and the influence of physical training«. Eur J Appl Physiol Occup Physiol; 37(4): 243-54.
33. Marinou K. Tousoulis D.Antonopoulos AS. Stefanadi E. Stefanadis C., (2010). »Obesity and cardiovascular disease: From pathophysiology to risk stratification«. Int J Cardiol; 138: 3–8.
34. McLaughlin T, Abbasi F, Lamendola C (2002). »Differentiation between obesity and insulin resistance in the association with C-reactive protein«. Circulation; 106: 2908-12.
35. Namazi A, Tarighhat esfanjani A, Heshmati J, Bahrami., A., (2011). »The effect of Hydro Alcholic Nettle Extracts on Insulin Sensitivity and some Inflammatory Indicators in patient with type2 diabetes«. Pakistan J 14(15):775-779.
36. Ramanjaneya, M., Chen, J., Brown, J.E., Tripathi, G., Hallschmid, M., Patel, S., et al., (2010). »Identification of nesfatin-1 in human and murine adipose tissue: a novel depot-specific adipokine with increased levels in obesity«. Endocrinology. Vol. 151, No.7,pp. 3169-80.
37. Retnakaran R, Hanley AJ, Raif N, Connelly PW, Sermer M, Zinman B., (2003). »C-reactive protein and gestational diabetes: the central role of maternal obesity«. J Clin Endocrinol Metab; 88: 3507-12.
38. Robergs, R.A., Landwehr, R., (2002). »The surprising history of the HR max = 220 – age equation«, Journal of Exercise Physiology online, Vol. 5, No. 2, PP. 1-10.
39. Selvin E, Paynter NP, Erlinger TP., (2007).» The effect of weight loss on C-reactive protein: a systematic review« .Arch Intern Med; 167: 31-39.
40. Shahraki M, MirshekariH, Shahraki A, Shahraki E., (2008). ».Effects of Urtica Dioica on glucose, insulin and lipid profile in diabetic rat«. Journal of Gonabad University of Medical Sciences; 3: 121-4.
41. Stewart LK, Flynn MG, Campbell WW, Craig BA, Robinson JP, Timmerman KL, et al., (2007).» The influence of exercise training on inflammatory cytokines and C-reactive protein«. Med Sci Sports Exerc; 39: 1714-9.
42. Stengel A, Goebel M, Wang L, Tache Y.,( 2010). »Ghre- lin, desacyl ghrelin and nesfatin-1 in gastric X/A- like cells«: Role as regulators of food intake and body weight. Peptides; 31: 357-369.
43. Stengel A, Goebel M, Yakubov I, Wang L, Witcher D, Coskun T, et al., (2009). »Identification and characterization of nesfatin-1 immunoreactivity in endocrine cell types of the rat gastric oxyntic mucosa«. Endocrinology; 150 (1): 232-238.
44. Stunkard AJ., (2003). »Depression and obesity«: Biological Psychiatry; 54(3): 330-7.
45. Tsuchiya, T., Shimizu, H., Yamada, M., Osaki, A., Oh-I, S., Ariyama, Y., et al., ,(2010).» Fasting concentrations of nesfatin-1 are negatively correlated with body mass index in non-obese males«. Clin endocrinol. Vol. 73,No.4,pp.484-90.
46. Vendrell J: Broch. M., Vilarrasa N Molina A., Gomez JM., Gutierrez C “ et al., . (2004). »Resistin adiponectin gherlin leptin and proinflammatory cytokines Relationship in Obesity«. “Obes Res “Vol 12 No 6. pp. 962-71.
47. Vieira, V.J., Valentine, R.J., Wilund, K.R., Antao, N., Baynard, T., Woods, J.A., (2009). »Effects of exercise and low-fat diet on adipose tissue inflammation and metabolic complications in obese mice«. Am J Physiol Endocrinol Metab. Vol.296, No.5,pp. 1164-71.
48. Weight LM, Alexander D, Jacobs P., (1991). »Strenuous exercise: analogous to the acute-phase response?«. Clin Sci (Lond); 81(5): 677-83.
49. Wong PC, Chia MY, Tsou IY, Wansaicheong GK, Tan B, Wang JC, et al., . (2008). »Effects of a 12-week exercise training programme on aerobic fitness, body composition, blood lipids and C-reactive protein in adolescents with obesity.« Ann Acad Med Singapore ; 37:286-93.
50. Yarnell E., (2005). »Stinging Nettle: A Modern View of an Ancient Healing Plant«. Alternative and Complementary Therapies; 2: 180-2.
51. Zargari A., (1996). »Medicinal plants, 6th ed, Tehran«, Tehran University of Medical Sciences; pp: 401-9. [in Persian].
52. Zare S, Nabiuni M, Tayanloo A, Hoseini S, Karimzadeh-Bardei L., (2015), »The effects of Urtica dioica extract on lipid profile, insulin resistance index and liver histology in polycystic ovary syndrome- induced Wistar rats«, Advanced Herbal Medicine; 1 (2): 23-33.