Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


1 MSc of Exercise Physiology, Semnan University, Semnan, Iran

2 Assistant Professor, Exercise Physiology Department, Semnan University, Semnan, Iran


Some evidence indicates that caffeine consumption before exercise may affect muscular damage. The aim of this study was to investigate the effects of caffeine consumption (6mg/kg) following a session of resistance exercise on creatine kinase (CK) and lactate dehydrogenase (LDH) in active men. 20 male physical education students (age 21.22±1.25 years, height 175.13±6.88 cm, weight 69.22±7.10 kg and BMI 22.21±2.21 kg/m2) were randomly assigned to placebo (n=10) and caffeine (n=10) groups. One hour before resistance exercise, subjects received caffeine or placebo in a double-blind manner. Resistance exercise included a circuit exercise in 5 stations (each station 3 sets); each set was performed by 75% 1RM in 8 to 10 repetitions. Blood samples were collected before, immediately after, 24 and 48 hours after the exercise. The results showed a significant increase in enzyme levels of CK (P=0.013) and LDH (P=0.001). However, these changes were not different between the two groups (P≥0.05). It seems that caffeine (6mg/kg) consumption before resistance exercise cannot reduce muscle damage indexes in blood.



1. آتشک، سیروان؛ شرفی، حسین؛ آذربایجانی، محمد­علی؛ گلی، محمد­امین؛ بتوراک، کاوه؛ کریمی، وریا. (1391). "تأثیر مکمل اسید چرب امگا-3 بر پراکسیداسیون لیپیدی و ظرفیت آنتی­اکسیدانی تام پلاسما متعاقب یک جلسه فعالیت مقاومتی در مردان ورزشکار جوان". مجلۀ علمی دانشگاه علوم پزشکی کردستان. دورۀ هفدهم، ص 59-51.
2. شیخ الاسلامی وطنی، داریوش؛ مرادی، آرزو. (1391). "پاسخ هورمونی، شاخص­های آسیب عضلانی و غلظت اسید­های آمینة پلاسما به‌دنبال فعالیت مقاومتی حاد همراه با مصرف مکمل BCAA". علوم زیستی ورزش. ش 15، ص 62-45.
3. کاشف، مجید؛ بنیان، عباس؛ راد، مرتضی. (1391). "تأثیر مصرف مکمل کراتین و مخلوط کراتین_کربوهیدرات بر توان بی­هوازی و شاخص­های آسیب سلولی (CK , LDH) در پسران ورزشکار 18-15 ساله". علوم زیستی ورزشی. ش 13، ص 152-125.
4. Avery NG, Kaiser JL, Sharman MJ, Scheett TP, Barnes DM, Gomez AL, et al. (2003). Effects of vitamin E supplementation on recovery from repeaded bouts of resistance exercise. Journal of Strength and Conditoning Research; 17(4): 801-809.
5. Bassini-Cameron A, Sweet E, Bottino A, Bittar C, Veiga C, Cameron LC. (2007). Effect of caffeine supplementation on haematological and biochemical variables in elite soccer players under physical stress conditions. Br. J. Sports Med; 41: 523-530.
6. Belviranli M, and Gokbel H. (2006). Acute Exercise Induced Oxidative Stress and Antioxidant Changes. European Journal of General Medicine; 3: 126-131.
7. Bloomer RJ, Flavo MJ, Schilling BK, and Smith, WA. (2007). Prior Exercise and Antioxidant Supplementation: Effect on Oxidative Stress and Muscle Injury. Journal of the International Society of Sports Nutrition; 4: 9-15.
8. Brzycki MA. (1995). Practical approach to strength training. 2th Edition. Indianapolis. Master Press; p: 62-65.
9. Cooper CE, Vollaard NBJ, Choueiri T, Wilson MT. (2002). Exercise, Free Radicals and Oxidative Stress. Biochemical Society Transactions; 30: 280-285.
10. Deminice R, Sicchieri T, Mialich MS, Milani F, Ovidio PP, Jordao AA (2011). Oxidative stress biomarker responses to an acute session of hypertrophy-resistance traditional interval training and circuit training. J Strength Cond Res; 25(3): 798-804.
11. Deminice R, Sicchieri T, Payão PO, Jordão AA (2010). Blood and salivary oxidative stress biomarkers following an acute session of resistance exercise in humans. International Journal of Sports Medicin; 31(9): 599-603.
12. Devasagayam TPA, Kamat JP, Mohan H, Kesavan PC. (1996). Caffeine as an antioxidant: inhibition of lipid peroxidation induced by reactive oxygen species. BBA Biomembranes; 1282(1): 63-70.
13. Evans WJ. (2000). Vitamin E, Vitamin C and Exercise. American Journal of Clinical Nutrition; 72: 647-652.
14. Greer, B.K. (2006). Dissertation: The effects of Branched-chain amino acid supplementation on indirect indicators of muscle damage and performance. ProQuest Information and Learning Company.
15. Güzel NV, Hazar S, Erbas D. (2007). Effects of different resistance exercise protocols on nitric oxide, lipid peroxidation and creatine kinase activity in sedentary males. J Sport Sci Med; 6: 417- 422.
16. Howatson G, van someren K. (2008). The prevention and treatment of exercise-induced muscle damage. Sports medicine; 38(6): 483-503.
17. jakson MJ. (1999). Freeradicalin skin and muscle: damaging agent or signals for adaptation? Proc nutr soc; 58: 673-676.
18. Krustrup P, Hellsten Y, Bangsbo J. (2004). Intense interval training enhances human skeletal muscle oxygen uptake in the initial phase of dynamic exercise high hut not at low intensities. J Physiol; 559(1): 335-345.
19. Lenn JON, Uhl T, Mattacola C, Boissonneault G, Yates J, Ibrahim W. (2002). The effects of fish oil and isoflavones on delayed onset muscle soreness. Med Sci Sports Exercise; 34: 1605-1613.
20. Liu JF, Chang WY, Chan KH, Tsai WY, Lin CL, Hsu MC. (2005). Blood lipid peroxides and muscle damage increased following intensive resistance training of female weightlifters. Ann N Y Acad Sci; 1042: 255-261.
21. Mahdavi R, Daneghian S, Homayouni A, Jafari A. (2012). Effects of Caffeine Supplementation on Oxidative Stress, Exercise-Induced Muscle Damage and Leukocytosis. Age (year); 24: 2-65.
22. Marco M, Anselmo CB, Marcio CX, Jarbas RS, José FFV. (2009). Caffeine Supplementation and muscle damage in soccer players. Brazilian Journal of Pharmaceutical Sciences; v45, n. 2, abr.
23. Mcanulty S, Mcanulty L, Nieman D, Morrow J, Utter A, Dumke C. (2005). Effect of resistance exercise and carbohydrate ingestion on oxidative stress Res; 39: 1219–1224.
24. McArdle WD, Katch FI, Katch VL. (2005), sport & exercise nutrition. Baltimore (MD): Lippincott Williams & Wilkins; (Series Editor).
25. McBride JM, Kraemer WJ, McBride TT, Sebastianelli W. (1998). Effect of resistance exercise on free radical production. Med Sci Sports Exer; 30: 67–72.
26. Motl RW, O’connor PJ, Dishman RK. (2003). Effect of caffeine on perceptions of leg muscle pain during moderate intensity cycling exercise. J Pain; 4: 316-321.
27. Nevin AG, Hazar S, Erbas D. (2007). Effect of different resistance exercise protocols on nitric oxide, lipid peroxidation and creatine kinase activity in sedentary males. Journal of sport science andmedicine; 6(41): 417-422.
28. Nieman DC, Henson DA, McAnulty L. (2002). Influence of vitamin C supplementation on oxidative and immune changes after an ultamarathon. J Appl Phyiol; 92: 1970-1977.
29. Pettersson J, et al. (2007). Muscular exercise can cause highlypathological liver function tests in healthy men. Br J Clin Pharmacol; 65(2): 253-259.
30. Atashak S, Baturak K. (2012). The Effect of BCAA supplementation on serum C – Reactive protein and Creatine Kinase after acute resistance exercise in soccer players. Annals of Biological Research; 3(3): 1569-1576.
31. Tokmakidis SP, Kokkinidis EA, Smilios I. (2003). The effects of ibuprofen on delayed muscle soreness and muscular performance after eccentric exercise. J Strength Cond. Res; 17: 53-59.
32. Vassilakopoulos T, Karatza MH, Katsaounou P, Kollinttza A, Zakynthinos S, Roussos C. (2003). Antioxidnts attenuate the plasma cytocine response to exercise in humans. J Appl Physiol; 94: 1025-1032.
33. Williams MH. (2004). Dietary Supplements and Sports Performance: Introduction and Vitamins. Journal of the International Society of Sports Nutrition; 1(2): 1-6.