The purpose of the present study was to investigate the effect of music tempo on heart rate and rate of perceived exertion of male physical education students. To this purpose, 45 male physical education students (height: 172.27 cm, SD=16.22, body mass index (BMI): 22.27kg/m², SD=3.08, weight: 68/05kg, SD=11.73, age: 21.93 years, SD=1.81) were randomly divided into control, high tempo (bpm=140) and low tempo experimental (bpm=100) groups and evaluated during during pretest and posttest. The subjects were requested to choose one piece of music from some pieces before the test (different pieces of music were exhibited for experimental groups). After 10 minutes of warm-up with and without music, subjects performed Wingate protocol. Heart rate and rate of perceived exertion were measured during 10 minutes of warm-up and Wingate protocol. Data were analyzed with SPSS and one-way ANOVA (P<0.05). The results of warm-up showed no significant differences in heart rate (P=0.001, F=24.00) and rate of perceived exertion (P=0.001, F=10.16) between low tempo music and control group, but these two indexes significantly increased in high tempo music group compared with low tempo music and control group. There were similar results about heart rate (P=0.001, F=12.25) and rate of perceived exertion (P=0.02, F=7.58) during Wingate protocol. It seems that listening to a piece of music before anaerobic performance affects heart rate and rate of perceived exertion.