The present study attempted to compare the structural and functional changes of left heart ventricle in female basketball players of Khuzestan superior league (aged between 18 and 29) during regular training and long-term detraining. Out of 30 female players, 15 subjects were assigned randomly to regular training group and 15 subjects to long-term detraining group. The structural and functional variables of left heart ventricle included end-systolic and end-diastolic internal diameter (LVEDS, LVEDD respectively), septum and posterior wall thickness (SWT, PWT respectively), left ventricular mass (LVM), stroke volume (SV), ejection fraction (EF) and rest heart rate (HR). These variables were studied and measured at rest by mono and two dimensional echocardiography and color Doppler technique. In order to analyze the collected data, descriptive statistics and multi-analysis of variance (MANOVA) were used (P<0.05). Findings of the study showed that regular training group enjoyed significantly higher structural and functional variables of left heart ventricle (except in the cases of end-systolic and posterior wall thickness) and lower rest heart rate compared with long-term detraining group. Overall results of this study revealed that left heart ventricular structure can positively be affected by basketball regular training.