Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


Department of Sport Sciences, Faculty of Humanities, Semnan University, Semnan, Iran.


Introduction: The objective of the current investigation was to examine alterations in the expression of the asprosin protein in the liver, pancreas, heart, and soleus muscle tissues of male diabetic rats following an eight-week period of endurance training and consumption of nettle supplements.

Methods: 48 male Wistar rats were randomly divided into 6 groups, control (n=8) (C), exercise (n=8) (E), diabetes (n=8) (D) and exercise+diabetes (n=8). (ED), nettle+diabetes (n=8) (UD), exercise+nettle+diabetes (n=8) (EUD). Following the induction of diabetes in rats via Strepotozocin injection, an aerobic continuous training with moderate to intense intensity, was implemented for a duration of 8 weeks, with a frequency of 5 days per week. Additionally, nettle extract was administered via intragastric gavage at a dosage of 150 mg per kilogram of body weight, 5 days per week for a period of 8 weeks.

Results: The results showed that in all tissues, asprosin was significantly increased in the diabetic patient group compared with the control group (P<0.05). The EUD group showed the greatest reduction compared with the diabetic group in all tissues compared to ED and EUD groups (P<0.05), so that in pancreatic tissue it reached the level of the healthy control group, but changes in cardiac tissue is negligible.

Conclusion: The expression of asprosin protein in the the liver, pancreas, heart and soleus muscle increases due to diabetes, and using a combination of endurance training and nettle extract can reduce it. The synergistic effect of combination endurance training and nettle were much better than either alone.


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