Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

1 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.

2 Corresponding Author, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

Abstract

Introduction: The current research aimed to measure the effects of one-month omega-3 supplement consumption on the most important symptoms of Delayed Onset Muscle Soreness (DOMS) in inactive healthy men.
Methods: The current research was a quasi-experimental and applied one in terms of purpose. The statistical population included inactive healthy men from Bukan City. Among them, 22 people were selected as a statistical sample and they were randomly assigned into two equal groups. Participants received a daily dose of 1000 mg omega-3 capsule or a 2% dextrose placebo for one month. After determining 1RM, participants performed the leg press in four sets of 20 repetitions using a leg press machine with a weight of 80% of 1RM. Each contraction lasted 4 seconds. Two ccs of venous blood were drawn by a specialist before the start of the study, 24, 48, and 72 hours after DOMS and measurements were made. For statistical analysis, independent t, repeated measures ANOVA, and Bonferroni's post hoc tests were used. A significance level of 0.05 was considered.
Results: Between-group comparison of PGE2, cortisol, pain perception and knee range of motion in all stages of the study in both control and supplement groups had significant differences (P<0.001). The results of the within-group test showed that the average PGE2, cortisol, and pain perception in the supplement group had a significant decrease in all post-intervention stages, while in the control group, there was a significant increase in all stages (P<0.05).
Conclusion: Taking omega-3 supplements can reduce the appearance, functional and biochemical symptoms of DOMS in the period after the completion of eccentric and concentric exercises; which can be due to the strong anti-inflammatory properties of omega-3 and the better response of the research participants to omega-3 consumption and better digestive absorption of this supplement.

Keywords

Main Subjects

[1] Ebrahim Kh, Rahmani Nia F, Talebi E. Investigating two methods of vitamin C consumption on the range of motion and extensor strength of the elbow flexors after delayed muscle contusion. Movement, 2001; 22;7(3):67-76. doi: articlepage/119540 [In Persian]

[2]  Arbabian S, et al. Effect of aqueous extract of saffron plant (Crocus sativus) on chronic pain caused by formalin test in small female laboratory rats. Kausar Medical Journal, 2009; 11-18. doi: article/52290 [In Persian]

[3]  Emam Ghoreyshi M, Ghasemi F. The effect of subchronic administration of aqueous and hydroalcoholic extracts of Fars Estehbanat saffron in the swimming test in mice. Armaghane danesh Journal, 2012; 10;16(6):527-36. doi: article-1-288-fa.html [In Persian]

[4]  Aminian Far A, et al. Investigating the effect of whole body vibration on the prevention and reduction of symptoms of delayed muscle irritation following eccentric exercises. KOOMESH, 2012;13(3 (43)):313-321. doi: 36938/fa [In Persian]

[5]  Hokmi M, Taghian F, Karimi A.  The effect of vibration on the prevention of delayed muscle contusion in active girls. Research in rehabilitation sciences, 2009; 5(2). doi: 10.22122/JRRS.V5I2.112 [In Persian]

[6]  Close GL, Ashton T, Cable T, Doran D, MacLaren DP. Eccentric exercise, isokinetic muscle torque and delayed onset muscle soreness: the role of reactive oxygen species. European journal of applied physiology. 2004;91(5):615-21. doi: 10.1007/s00421-003-1012-2.

[7]  Akbarnejad A, Rajabi A, Yari M, Mamshali E. The Effect of Saffron Consumption and Intermittent Aerobic Exercise on Spirometryand Physiological Parametersand Blood Pressure in Non-Athletic Boys. Sport Physiology & Management Investigations. 2017;9(2):21-33.

[8]  Lenn J, Uhl T, Mattacola C, Boissonneault G, Yates J, Ibrahim W, et al. The effects of fish oil and isoflavones on delayed onset muscle soreness. Medicine and Science in Sports and Exercise. 2002;34(10):1605-13. doi: 10.1097/00005768-200210000-00012

[9]  Zeinali S, Reza-Nejad S, Marandi SM, Khayam-Bashi KH. Study of the Effectiveness of Vibration in Reduction of Delayed Onset Muscle Soreness Resulting after Therapeutic Exercise. Journal of Shahid Sadoughi University of Medical Sciences and Healthcare Services, Yazd. 2009; 17 (3) :184-192. doi: article-1-1749-fa.html [In Persian]

[10]Vakili A, Eynali MR, Bandegi AR. The protective effects of Saffron against the oxidative damage in a transient model of focal cerebral ischemia in rats. Tehran Univ Med J. 2011; 69 (7) :405-412. doi: article-1-210-fa.html [In Persian]

[11]Akbarnejad A, Rajabi A, Yari M, Mamshali EJSP, Investigations M. The effect of saffron consumption and intermittent aerobic exercise on spirometryand physiological parametersand blood pressure in non-athletic boys. 2017;9(2):21-33.

[12]Meamarbashi A, Rajabi AJCJoSM. Preventive effects of 10-day supplementation with saffron and indomethacin on the delayed-onset muscle soreness. 2015;25(2):105-12. doi: 10.1097/JSM.0000000000000113 [In Persain]

[13]Murphy RC, Hammarström S, Samuelsson BJPotNAoS. Leukotriene C: a slow-reacting substance from murine mastocytoma cells. 1979;76(9):4275-9. doi: 10.1073/pnas.76.9.4275

[14]Castellano V, Patel DI, White LJJJoAP. Cytokine responses to acute and chronic exercise in multiple sclerosis. 2008;104(6):1697-702. doi: 10.1152/japplphysiol.00954.2007

[15]Choobine S, Akbarnejad A, Borjian M, Kordi MRJJoSB. The effect of Omega-3 supplementation on serum prostaglandin E2 in athlete women after a single bout of exhaustive exercise. 2013;4(15):121-33. doi: 10.22059/jsb.2013.29782 [In Persian]

[16]Daryanoosh F, Mehrabani DJJoSB. Evaluating inflammatory index changes and muscle injuries in male mice after 8 weeks of aerobic exercise and Omega-3 consumption. 2012;4(10):77-94. doi: 10.22059/jsb.2012.21999 [In Persian]

[17]Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Molecular biology or the cell.  Molecular biology or the cell2002.

[18]Anderson SD, Daviskas EJJoa, immunology c. The mechanism of exercise-induced asthma is…. 2000;106(3):453-9. doi: 10.1067/mai.2000.109822

[19]Giaccio M. Crocetin from saffron: an active component of an ancient spice. Critical reviews in food science and nutrition. 2004;44(3):155-72. doi: 10.1080/10408690490441433

[20]Gibson W, Arendt-Nielsen L, Graven-Nielsen T. Delayed onset muscle soreness at tendon–bone junction and muscle tissue is associated with facilitated referred pain. Experimental brain research. 2006;174(2):351-60. doi: 10.1007/s00221-006-0466-y

[21]Haghighi-Zad H, et al. The protective effect of saffron aqueous extract on inhibition of learning and spatial memory induced by morphine in rats. Physiology and Pharmacology. 2008; 12(3)، 170-179. doi: paper/596355 [In Persian]

[22]Abramson S, Korchak H, Ludewig R, Edelson H, Haines K, Levin RI, et al. Modes of action of aspirin-like drugs. Proceedings of the National Academy of Sciences. 1985;82(21):7227-31. doi: 10.1073/pnas.82.21.7227

[23]Ahmadizad S, El-Sayed MS. The acute effects of resistance exercise on the main determinants of blood rheology. Journal of sports sciences. 2005;23(3):243-9. doi: 10.1080/02640410410001730151

[24]Åstrand P-O, Rodahl K, Dahl H, Strà SB. Textbook of work physiology: physiological bases of exercise: Human kinetics; 2003.

[25]Bosco C, Colli R, Bonomi R, von Duvillard SP, Viru AJM, sports si, et al. Monitoring strength training: neuromuscular and hormonal profile. 2000;32(1):202-8. doi: 10.1097/00005768-200001000-00030

[26]Kraemer WJ, Häkkinen K, Newton RU, Nindl BC, Volek JS, McCormick M, et al. Effects of heavy-resistance training on hormonal response patterns in younger vs. older men. 1999;87(3):982-92. doi: 10.1152/jappl.1999.87.3.982

[27]Tartibian B, Azadpoor N. The effect of exercise intensity on inflammatory indices and muscle damage in young non-athletes. Human movement science. 2008; 23;1(1):33-42. doi:  view/fa/articlepage/369995 [In Persian]

[28]Zarrindast M-R, Dinkoub Z, Homayoun H, Bakhtiarian A, Khavandgar S. Dopamine receptor mechanism (s) and morphine tolerance in mice. Journal of Psychopharmacology. 2002;16(3):261-6. 10.1177/026988110201600312

[29]Taiwo Y, Levine J. Further confirmation of the role of adenyl cyclase and of cAMP-dependent protein kinase in primary afferent hyperalgesia. Neuroscience. 1991;44(1):131-5. doi: 10.1016/0306-4522(91)90255-m

[30]Sayers SP, Dannecker EA. How to prevent delayed onset muscle soreness (DOMS) after eccentric exercise. International SportMed Journal. 2004;5(2):84-97. doi: 10520/EJC48550

[31]Almekinders LC. Anti-inflammatory treatment of muscular injuries in sport. Sports Medicine. 1999;28(6):383-8.

[32]Tartibian B, Aziz-beigi-Bokani K. The effect of naproxen consumption on perceived pain intensity and changes in creatine kinase enzyme levels following eccentric exercises. Movement. 2008, 77-92. doi: view/fa/articlepage/339535 [In Persian]

[33]Meamarbashi A, Rajabi A. A Comparative Study on the Antinociceptive and Anti-Inflammatory Effects of Saffron and Indomethacin in the Prevention of Delayed Onset Muscle Soreness (DOMS). Journal of Sport Biosciences. 2015, 22;7(4):541-61. doi: 10.22059/jsb.2015.57281 [In Persian]

[34]Jamal Sh, et al. Reducing expression of tolerance to morphine by aqueous extract of saffron plant (Crocus sativus) in small female laboratory rats by Tail Flick method. Congress of Physiology and Pharmacology of Iran. 2007; 197-210. doi: paper/806970/fa [In Persian]

[35]Barlas P, Craig JA, Robinson J, Walsh DM, Baxter GD, Allen JM. Managing delayed-onset muscle soreness: lack of effect of selected oral systemic analgesics. Archives of physical medicine and rehabilitation. 2000;81(7):966-72. doi: 10.1053/apmr.2000.6277

[36]Law RY, Herbert RD. Warm-up reduces delayed-onset muscle soreness but cool-down does not: a randomised controlled trial. Australian Journal of Physiotherapy. 2007;53(2):91-5. doi: 10.1016/S0004-9514(07)70041-7

[37]Vahidi A, Bashardost N, Akhondi H. The analgesic effect of saffron extract in rats as compared with morphine sulfate. Planta Medica. 2007;73(09):P_552. doi: 10.1055/s-2007-987332 [In Persian]

[38]Peterson JM, Trappe TA, Mylona E, White F, Lambert CP, Evans WJ, et al. Ibuprofen and acetaminophen: effect on muscle inflammation after eccentric exercise. Medicine and science in sports and exercise. 2003;35(6):892-6. doi: 10.1249/01.MSS.0000069917.51742.98