The Effect of Two Types of Aerobic Training (Continuous and Interval) and Vanadium-Zinc Complex Supplementation on Antioxidant Genes Expression in the Testicular Tissue of Rats Fed With Fructose Solution

Hamedchaman, Nader; Safarzade, Alireza; Hamidian, Gholamreza; Nasiri, Khadijeh

doi:10.22059/jsb.2023.361255.1594

Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

¹ Department of Exercise Physiology, Faculty of Sports Sciences, University of Mazandaran, Babolsar, Iran.

² Corresponding Author, Department of Exercise Physiology, Faculty of Sports Sciences, University of Mazandaran, Babolsar, Iran.

³ Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.

⁴ . Department of Exercise Physiology, Faculty of Sports Sciences, University of Mazandaran, Babolsar, Iran.

https://doi.org/10.22059/jsb.2023.361255.1594

Abstract

Introduction: Excessive fructose consumption induces insulin resistance and elevates oxidative stress levels in various body tissues, including the testicular tissue. The present study aimed to investigate the effects of aerobic training (continuous and interval) and vanadium-zinc supplementation on the expression of antioxidant genes in testicular tissue in rats fed with fructose syrup.
Methods: fifty-six male rats were divided into seven groups including normal control (NC), fructose control (FC), moderate-intensity continuous training (FCT), high-intensity interval training (FIT), vanadium-zinc supplement (FS), moderate-intensity continuous training+vanadium-zinc supplement (FSCT) and high-intensity interval training+vanadium-zinc supplement (FSIT). The GPX, SOD, and CAT genes expression in testicular tissue and serum levels of TAC, TOC, glucose, insulin, and HOMA-IR were measured. One-way ANOVA and Bonferroni post-hoc tests were used for data analysis at a significant level of P≤0.05.
Results: Sixteen weeks of fructose syrup consumption led to a significant increase in serum glucose, HOMA-IR, and TOC levels and a significant decrease in serum TAC, and the levels of GPX, SOD, and CAT gene expression in the testicular tissue of the FC group compared with NC group (P<0.05). Eight weeks of vanadium-zinc supplementation and aerobic training (continuous and interval) were associated with a decrease in serum glucose, HOMA-IR, and TOC levels and an increase in serum TAC and levels of GPX, SOD, and CAT gene expression compared with the FC group (P<0.05).
Conclusion: The results of this research showed that aerobic training (continuous and interval) and vanadium-zinc supplementation improve the metabolic status and subsequently improve the oxidative status in subjects suffering from insulin resistance.

The Effect of Two Types of Aerobic Training (Continuous and Interval) and Vanadium-Zinc Complex Supplementation on Antioxidant Genes Expression in the Testicular Tissue of Rats Fed With Fructose Solution

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Volume 15, Issue 4 - Serial Number 98December 2023Pages 21-38

Volume 15, Issue 4 - Serial Number 98
December 2023
Pages 21-38