Comparing Endurance and Resistance training on the Expression of Senescence-Related Genes in the Visceral Adipose Tissue of obese rats

Ghorbani Dasht Bayaz, Nastaran; Donyaie, Adel; Vosadi, Elham

doi:10.22059/jsb.2023.351533.1560

Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

¹ Faculty of Physical Education and sport sciences, Shahrood University of Technology, Semnan, Iran.

² assistant professor of Shahrood University of Technology

³ Assistant Professor of Sport Physiology Shahro University of Technology

https://doi.org/10.22059/jsb.2023.351533.1560

Abstract

Background: Positive regulation of senescence-related markers leads to adipose tissue disorders. Exercise seems to improve aging in adipose tissue.

Aims: The aim of this study was to compare the effect of endurance and resistance training on the expression of senescence-related genes.

Methods: In this experimental study, Twenty-four obese rats were divided into three groups: (1) Endurance Training, (2) Resistance Training, and (3) Control groups. Training groups trained for 8 weeks and 5 days a week. The Endurance group exercise program consists of Running on a treadmill and the Resistance group exercise program is included Climbing a resistance ladder. While, the control group had no training. The expression of P53, P16, and IL-6 genes in visceral tissue of obese rats were measured by Real-time PCR analysis. Finally, the data were analyzed by one-way ANOVA and Tukey post hoc test and statistical differences were considered significant at P<0.05.

Results: The results of this study showed the expression levels of P53, P16, and IL-6 genes in the Endurance Training group (P=0.001, P=0.006, P=0.001) and Resistance Training group (P=0.001, P=0.038, P=0.001) had a significant decrease compared to the control group. There was also no significant difference in the expression of P53, P16, and IL-6 genes between the endurance and resistance groups (P=0.439, P=0.584, P=0.300).

Conclusion: According to the results of the present study, it seems that endurance and resistance training plays a role in reducing the expression of senescence-related genes, which This effect was no different in endurance and resistance training.

Keywords

Main Subjects

Exercise Physiology

References

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[19] Dashtiyan AA, Sepehrimanesh M, Tanideh N, Afzalpour ME. The effect of endurance training with and without vitamin E on expression of p53 and PTEN tumor suppressing genes in prostate glands of male rats. Biochimie open. 2017 Jun 1; 4:112-8. [In Persian] Doi: 10.1016/j.biopen.2017.03.005

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[21] Schafer MJ, White TA, Evans G, Tonne JM, Verzosa GC, Stout MB, Mazula DL, Palmer AK, Baker DJ, Jensen MD, Torbenson MS. Exercise prevents diet-induced cellular senescence in adipose tissue. Diabetes. 2016 Jun 1;65(6):1606-15. Doi: 10.2337/db15-0291

[22] Yang C, Jiao Y, Wei B, Yang Z, Wu JF, Jensen J, Jean WH, Huang CY, Kuo CH. Aged cells in human skeletal muscle after resistance exercise. Aging (Albany NY). 2018 Jun;10(6):1356. Doi: 10.18632/aging.101472

[23] Chen XK, Yi ZN, Wong GT, Hasan KM, Kwan JS, Ma AC, Chang RC. Is exercise a senolytic medicine? A systematic review. Aging Cell. 2021 Jan;20(1): e13294. Doi: 10.1111/acel.13294

[24] Libardi CA, De Souza GV, Cavaglieri CR, Madruga VA, Chacon-Mikahil M. Effect of resistance, endurance, and concurrent training on TNF-a, IL-6, and CRP. Med Sci Sports Exerc. 2012;44(1):50-6. Doi: 10.1249/mss.0b013e318229d2e9

[25] Prestes J, Shiguemoto G, Botero JP, Frollini A, Dias R, Leite R, Pereira G, Magosso R, Baldissera V, Cavaglieri C, Perez S. Effects of resistance training on resistin, leptin, cytokines, and muscle force in elderly post-menopausal women. Journal of sports sciences. 2009 Dec 1;27(14):1607-15. Doi: 10.1080/02640410903352923

[26] Melancon MO, Lorrain D, Dionne IJ. Changes in markers of brain serotonin activity in response to chronic exercise in senior men. Applied Physiology, Nutrition, and Metabolism. 2014;39(11):1250-6. Doi: 10.1139/apnm-2014-0092

Journal of Sport Biosciences

Comparing Endurance and Resistance training on the Expression of Senescence-Related Genes in the Visceral Adipose Tissue of obese rats

References

References

[1] Baker DJ, Wijshake T, Tchkonia T, LeBrasseur NK, Childs BG, Van De Sluis B, Kirkland JL, Van Deursen JM. Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders. Nature. 2011 Nov;479(7372):232-6. Doi: 10.1038/nature10600

[2] De Carvalho FG, Justice JN, Freitas EC, Kershaw EE, Sparks LM. Adipose tissue quality in aging: how structural and functional aspects of adipose tissue impact skeletal muscle quality. Nutrients. 2019 Oct 23;11(11):2553. Doi: 10.3390/nu11112553

[3] Ogrodnik M, Zhu YI, Langhi LG, Tchkonia T, Krüger P, Fielder E, Victorelli S, Ruswhandi RA, Giorgadze N, Pirtskhalava T, Podgorni O. Obesity-induced cellular senescence drives anxiety and impairs neurogenesis. Cell metabolism. 2019 May 7;29(5):1061-77. Doi: 10.1016/j.cmet.2018.12.008.

[5] Gorgoulis V, Adams PD, Alimonti A, Bennett DC, Bischof O, Bishop C, Campisi J, Collado M, Evangelou K, Ferbeyre G, Gil J. Cellular senescence: defining a path forward. Cell. 2019 Oct 31;179(4):813-27. Doi: 10.1016/j.cell.2019.10.005

[8] Mijit M, Caracciolo V, Melillo A, Amicarelli F, Giordano A. Role of p53 in the Regulation of Cellular Senescence. Biomolecules. 2020 Mar 8;10(3):420. Doi:10.3390/biom10030420

[9] Dang Y, An Y, He J, Huang B, Zhu J, Gao M, Zhang S, Wang X, Yang B, Xie Z. Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression. Aging Cell. 2020 Jan;19(1):e13060. Doi: 10.1111/acel.13060

[10] Kern L, Mittenbühler MJ, Vesting AJ, Ostermann AL, Wunderlich CM, Wunderlich FT. Obesity-induced TNFα and IL-6 signaling: the missing link between obesity and inflammation—driven liver and colorectal cancers. Cancers. 2018 Dec 27;11(1):24. Doi: 10.3390/cancers 11010024

[13] Lee S, Farrar RP. Resistance training induces muscle-specific changes in muscle mass and function in rat. Journal of Exercise physiology online. 2003 May 1;6(2).

[15] Iemitsu M, Maeda S, Miyauchi T, Matsuda M, Tanaka H. Gene expression profiling of exercise‐induced cardiac hypertrophy in rats. Acta Physiologica Scandinavica. 2005 Dec;185(4):259-70. Doi: 10.1111/j.1365-201x.2005. 01494.x

[16] Askari B, bijeh N, rashidlamir A. The effect of 8 weeks IGF-1 injection and resistance training on Cox-2 and p53 expression in colorectal of male Wistar rats. Gournal of Sport and Exercise Physiology. 2019; 12(1):115-27. (In Persian) Doi: 10.52547/joeppa.12.1.115

[17] Beyfuss K, Hood DA. A systematic review of p53 regulation of oxidative stress in skeletal muscle. Redox Report. 2018 Jan 1;23(1):100-17. Doi: 10.1080/13510002.2017.1416773

[18] Qi Z, He J, Zhang Y, Shao Y, Ding S. Exercise training attenuates oxidative stress and decreases p53 protein content in skeletal muscle of type 2 diabetic Goto-Kakizaki rats. Free radical biology and medicine. 2011 Apr 1;50(7):794-800. Doi: 10.1016/j.freeradbiomed.2010.12.022

[19] Dashtiyan AA, Sepehrimanesh M, Tanideh N, Afzalpour ME. The effect of endurance training with and without vitamin E on expression of p53 and PTEN tumor suppressing genes in prostate glands of male rats. Biochimie open. 2017 Jun 1; 4:112-8. [In Persian] Doi: 10.1016/j.biopen.2017.03.005

[20] Shirvani H, Isanejad A, Rahim M, Bazgir B, Alizadeh AM. Changes in monocarboxylate transporter 1 and p53 gene expression by aerobic interval training in the experimental colon carcinoma of mouse. Tehran University Medical Journal. 2018 Aug 15;76(5). [In Persian]

[21] Schafer MJ, White TA, Evans G, Tonne JM, Verzosa GC, Stout MB, Mazula DL, Palmer AK, Baker DJ, Jensen MD, Torbenson MS. Exercise prevents diet-induced cellular senescence in adipose tissue. Diabetes. 2016 Jun 1;65(6):1606-15. Doi: 10.2337/db15-0291

[22] Yang C, Jiao Y, Wei B, Yang Z, Wu JF, Jensen J, Jean WH, Huang CY, Kuo CH. Aged cells in human skeletal muscle after resistance exercise. Aging (Albany NY). 2018 Jun;10(6):1356. Doi: 10.18632/aging.101472

[23] Chen XK, Yi ZN, Wong GT, Hasan KM, Kwan JS, Ma AC, Chang RC. Is exercise a senolytic medicine? A systematic review. Aging Cell. 2021 Jan;20(1): e13294. Doi: 10.1111/acel.13294

[24] Libardi CA, De Souza GV, Cavaglieri CR, Madruga VA, Chacon-Mikahil M. Effect of resistance, endurance, and concurrent training on TNF-a, IL-6, and CRP. Med Sci Sports Exerc. 2012;44(1):50-6. Doi: 10.1249/mss.0b013e318229d2e9

[26] Melancon MO, Lorrain D, Dionne IJ. Changes in markers of brain serotonin activity in response to chronic exercise in senior men. Applied Physiology, Nutrition, and Metabolism. 2014;39(11):1250-6. Doi: 10.1139/apnm-2014-0092

Volume 15, Issue 3 - Serial Number 97
September 2023
Pages 51-60

Comparing Endurance and Resistance training on the Expression of Senescence-Related Genes in the Visceral Adipose Tissue of obese rats

References

References

[1] Baker DJ, Wijshake T, Tchkonia T, LeBrasseur NK, Childs BG, Van De Sluis B, Kirkland JL, Van Deursen JM. Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders. Nature. 2011 Nov;479(7372):232-6. Doi: 10.1038/nature10600

[2] De Carvalho FG, Justice JN, Freitas EC, Kershaw EE, Sparks LM. Adipose tissue quality in aging: how structural and functional aspects of adipose tissue impact skeletal muscle quality. Nutrients. 2019 Oct 23;11(11):2553. Doi: 10.3390/nu11112553

[3] Ogrodnik M, Zhu YI, Langhi LG, Tchkonia T, Krüger P, Fielder E, Victorelli S, Ruswhandi RA, Giorgadze N, Pirtskhalava T, Podgorni O. Obesity-induced cellular senescence drives anxiety and impairs neurogenesis. Cell metabolism. 2019 May 7;29(5):1061-77. Doi: 10.1016/j.cmet.2018.12.008.

[5] Gorgoulis V, Adams PD, Alimonti A, Bennett DC, Bischof O, Bishop C, Campisi J, Collado M, Evangelou K, Ferbeyre G, Gil J. Cellular senescence: defining a path forward. Cell. 2019 Oct 31;179(4):813-27. Doi: 10.1016/j.cell.2019.10.005

[8] Mijit M, Caracciolo V, Melillo A, Amicarelli F, Giordano A. Role of p53 in the Regulation of Cellular Senescence. Biomolecules. 2020 Mar 8;10(3):420. Doi:10.3390/biom10030420

[9] Dang Y, An Y, He J, Huang B, Zhu J, Gao M, Zhang S, Wang X, Yang B, Xie Z. Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression. Aging Cell. 2020 Jan;19(1):e13060. Doi: 10.1111/acel.13060

[10] Kern L, Mittenbühler MJ, Vesting AJ, Ostermann AL, Wunderlich CM, Wunderlich FT. Obesity-induced TNFα and IL-6 signaling: the missing link between obesity and inflammation—driven liver and colorectal cancers. Cancers. 2018 Dec 27;11(1):24. Doi: 10.3390/cancers 11010024

[13] Lee S, Farrar RP. Resistance training induces muscle-specific changes in muscle mass and function in rat. Journal of Exercise physiology online. 2003 May 1;6(2).

[15] Iemitsu M, Maeda S, Miyauchi T, Matsuda M, Tanaka H. Gene expression profiling of exercise‐induced cardiac hypertrophy in rats. Acta Physiologica Scandinavica. 2005 Dec;185(4):259-70. Doi: 10.1111/j.1365-201x.2005. 01494.x

[16] Askari B, bijeh N, rashidlamir A. The effect of 8 weeks IGF-1 injection and resistance training on Cox-2 and p53 expression in colorectal of male Wistar rats. Gournal of Sport and Exercise Physiology. 2019; 12(1):115-27. (In Persian) Doi: 10.52547/joeppa.12.1.115

[17] Beyfuss K, Hood DA. A systematic review of p53 regulation of oxidative stress in skeletal muscle. Redox Report. 2018 Jan 1;23(1):100-17. Doi: 10.1080/13510002.2017.1416773

[18] Qi Z, He J, Zhang Y, Shao Y, Ding S. Exercise training attenuates oxidative stress and decreases p53 protein content in skeletal muscle of type 2 diabetic Goto-Kakizaki rats. Free radical biology and medicine. 2011 Apr 1;50(7):794-800. Doi: 10.1016/j.freeradbiomed.2010.12.022

[19] Dashtiyan AA, Sepehrimanesh M, Tanideh N, Afzalpour ME. The effect of endurance training with and without vitamin E on expression of p53 and PTEN tumor suppressing genes in prostate glands of male rats. Biochimie open. 2017 Jun 1; 4:112-8. [In Persian] Doi: 10.1016/j.biopen.2017.03.005

[20] Shirvani H, Isanejad A, Rahim M, Bazgir B, Alizadeh AM. Changes in monocarboxylate transporter 1 and p53 gene expression by aerobic interval training in the experimental colon carcinoma of mouse. Tehran University Medical Journal. 2018 Aug 15;76(5). [In Persian]

[21] Schafer MJ, White TA, Evans G, Tonne JM, Verzosa GC, Stout MB, Mazula DL, Palmer AK, Baker DJ, Jensen MD, Torbenson MS. Exercise prevents diet-induced cellular senescence in adipose tissue. Diabetes. 2016 Jun 1;65(6):1606-15. Doi: 10.2337/db15-0291

[22] Yang C, Jiao Y, Wei B, Yang Z, Wu JF, Jensen J, Jean WH, Huang CY, Kuo CH. Aged cells in human skeletal muscle after resistance exercise. Aging (Albany NY). 2018 Jun;10(6):1356. Doi: 10.18632/aging.101472

[23] Chen XK, Yi ZN, Wong GT, Hasan KM, Kwan JS, Ma AC, Chang RC. Is exercise a senolytic medicine? A systematic review. Aging Cell. 2021 Jan;20(1): e13294. Doi: 10.1111/acel.13294

[24] Libardi CA, De Souza GV, Cavaglieri CR, Madruga VA, Chacon-Mikahil M. Effect of resistance, endurance, and concurrent training on TNF-a, IL-6, and CRP. Med Sci Sports Exerc. 2012;44(1):50-6. Doi: 10.1249/mss.0b013e318229d2e9

[26] Melancon MO, Lorrain D, Dionne IJ. Changes in markers of brain serotonin activity in response to chronic exercise in senior men. Applied Physiology, Nutrition, and Metabolism. 2014;39(11):1250-6. Doi: 10.1139/apnm-2014-0092

Volume 15, Issue 3 - Serial Number 97September 2023Pages 51-60

Volume 15, Issue 3 - Serial Number 97
September 2023
Pages 51-60