Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license
1 Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
2 Department of Physical Education and Sport Science, Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran.
The ubiquitin pathway is responsible for muscle atrophy; Atrogin-1 or muscle atrophy F-box (MAFbx) and muscle ring-finger protein 1 (MuRF) proteins are important factors in this pathway. This study aims to investigate the effect of endurance training on the intracellular content of proteins related to the Ubiquitin-Proteasome pathway in the left ventricular of the heart of rats with type 2 diabetes. In this experimental study, 18 rats 3-month-old male Sprague-Dawley with a mean weight of 270±20 g were selected. 12 rats became diabetic by intraperitoneal injection of Streptozotocin and nicotinamide solutions. These rats were randomly divided into 2 groups: diabetic training and diabetic control; A healthy control group (each group of six rats) was also considered. The training group trained endurance training 4 days a week for 8 weeks. Data were analyzed using SPSS software version 23 and one-way ANOVA and Tukey post hoc tests. MAFbx protein content showed a significant change after eight weeks of endurance training (P=0.0001); Tukey's post hoc test showed that this is a significant change between the pairs of diabetic training groups compared to diabetic control (decrease) (P=0.0001) and diabetic training groups compared to healthy control (increase) (P=0.004). MuRF1 protein content showed a significant decrease (P=0.0001); This reduction was significant between pairs of diabetic training groups compared to diabetic control (P=0.0001). Endurance training can prevent atrophy and lead to proper left ventricular function by reducing the content of MAFbx and MuRF1 proteins in the heart of diabetic subjects.