Sport Biosciences

The Effect of Endurance Training on the Intracellular Content of Proteins Related to the Ubiquitin-Proteasome Pathway in the Left Ventricle of Type-2 Diabetic Rats

Document Type : Research Paper

Authors

Abdol Nasser Seidi 1
Neda Aghaei Bahmanbeglou 1
Habib Asgharpour 1
Mozhgan Ahmadi 2

1 Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.

2 Department of Physical Education and Sport Science, Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran.

https://doi.org/10.22059/jsb.2023.345403.1544
Abstract
Introduction: The Ubiquitin pathway is responsible for muscle atrophy; the Atrogin-1 or muscle atrophy F-box (MAFbx) and the muscle Ring-Finger protein-1 (MuRF1) are the important factors in this pathway. This study aimed to investigate the effect of endurance training on the intracellular content of proteins related to the Ubiquitin-Proteasome pathway in the left ventricular tissue of rats with type-2 diabetes.
 Methods: In this experimental study, 18 three-month-old male Sprague-Dawley rats with a mean weight of 270±20 grams were selected. Type-2 diabetes was induced in 12 rats by intraperitoneal injection of streptozotocin and nicotinamide solutions. These rats were randomly divided into two diabetic training and diabetic control groups. A healthy control group (six rats in each group) was also considered. The training group did endurance training four days a week according to the training program for eight weeks. Data were analyzed using one-way ANOVA and Tukey post hoc tests via SPSS software version 23.
Results: MAFbx protein content showed a significant change after eight weeks of endurance training (P=0.0001); Tukey's post hoc test showed that this significant change was between pairs of the diabetic training compared with diabetic control (decrease) groups (P=0.0001)  and diabetic training compared with the healthy control (increase) groups (P=0.004). MuRF1 protein content showed a significant decrease (P=0.0001); This reduction was significant between pairs of diabetic training compared with diabetic control groups (P=0.0001).
Conclusion: Endurance training can prevent atrophy and lead to proper left ventricular function by reducing the content of MAFbx and MuRF1 proteins in the heart of diabetic rats.

Keywords

Endurance training
Left ventricle
Muscle atrophy F-Box protein
Muscle Ring-Finger protein-1
Type-2 diabetes
Subjects
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  • Receive Date 04 July 2022
  • Revise Date 11 February 2023
  • Accept Date 01 March 2023

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