The effect of spinal nerve ligation after the combined and resistance training on the MST1 and MAFbx genes expression in Plantaris muscle and behavioral tests in male Wistar rats

Document Type : Research Paper I Open Access I Released under CC BY 4.0 license


1 Associate Prof, Dept of Physical Education, Faculty of Letters and Humanities, Vali E Asr University, Rafsanjan, Iran.

2 دانشجوی کارشناسی ارشد، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، کرمان، ایران.


. 24 8-week-old male rats with the mean weight of 250± 20 g were randomly assigned into 3 groups: 1) Sham-Spinal Nerve Ligation group (Sham-SNL): Three branches of rat sciatic nerve tightly were tied. 2) Resistance Training - Spinal Nerve Ligation group (SNL-S): The three sciatic nerve branches of the rats were tightly were tied after a period of strength training. 3) Combined training Group-Spinal Nerve Ligation (SNL-CO): Three branches of the sciatic nerve of rats were tightly were tied after of the endurance-resistance training. To create the SNL, in the first the rats were anesthetized with Xylazine -ketamine and then their fifth lumbar spinal nerve was tightly tied using the Kim and Chang method. The duration of the SNL protocol was 4 weeks. At the end of the protocol, the rats were sacrificed and the Plantaris muscle was extracted. MST1 and MAFbx gene expression in the Sham-SNL group was non-significantly higher than the resistance and combination training groups (P <0.05). These results show that spinal nerve ligation after of the resistance and combined training groups has no significant effect on MAFbx and MST1 gene expression in Plantaris muscle of male Wistar rats, but based on the significant changes in behavioral tests in the training groups group compared to the Sham-SNL, it can be claimed that resistance and combination training can be useful for improving neuropathic pain.


Main Subjects

  • Receive Date: 21 November 2021
  • Revise Date: 07 February 2022
  • Accept Date: 20 February 2022
  • First Publish Date: 05 March 2022