Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license
1 . MSc of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran
2 Assistant Professor of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran
Aging is often associated with a progressive loss of muscle strength and an increase in fat mass. High-intensity interval training may improve muscle strength, but remains largely unstudied in ageing participants. Therefore, the purpose of this study was to determine the effects of eight weeks of low-frequency high-intensity interval and L-citrulline supplementation on myostatin, aerobic power, muscular strength and endurance and body fat in older men. 30 inactive old men randomly divided into three groups: high-intensity interval training (HIT), citrolin (CIT) and high-intensity interval training+citrolin (HIT+CIT). HIIT was performed for 8 weeks and 2 sessions per week that included 30 seconds cycling exercise on ergometer (intensity of 85-90% HR reserve). CIT supplement consumed in single 8 g-dose alternateve day. Results showed that there was significant decrease for myostatin in HIT+CIT and for body fat in HIT+CIT and HIT than CIT. Muscular strength and aerobic power significant increase in HIT+CIT and HIT than CIT. HIT+CITgroup showed significant increase compared to CIT. In conclusion, low-frequency HIIT alone and combined with L-citrulline seems to induce greater improvements in muscular strength and mass factors and aerobic performance in inactive elderly men. Therefore, low-frequency HIIT+CIT can be considered as effective program to prevent or reduce sarcopenia among elderly.
- 1. Bouchard DR, Janssen I. Dynapenic-obesity and physical function in older adults. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences. 2010;65(1):71-77.
- Stenholm S, Alley D, Bandinelli S, Griswold M, Koskinen S, Rantanen T, et al. The effect of obesity combined with low muscle strength on decline in mobility in older persons: results from the InCHIANTI study. International journal of obesity. 2009;33(6):635-644.
- Harvey JA, Chastin SF, Skelton DA. Prevalence of sedentary behavior in older adults: a systematic review. International journal of environmental research and public health. 2013;10(12):6645-6661.
- Gibala MJ, Little JP, MacDonald MJ, Hawley JA. Physiological adaptations to low‐volume, high‐intensity interval training in health and disease. The Journal of physiology. 2012;590(5):1077-1084.
- Tschopp M, Sattelmayer MK, Hilfiker R. Is power training or conventional resistance training better for function in elderly persons? A meta-analysis. Age and ageing. 2011;40(5):549-56.
- Gillen JB, Gibala MJ. Interval training: a time-efficient exercise strategy to improve cardiometabolic health. Applied Physiology, Nutrition, and Metabolism. 2018;43(10):26-41.
- Coetsee C, Terblanche E. The effect of three different exercise training modalities on cognitive and physical function in a healthy older population. European Review of Aging and Physical Activity. 2017;14(1):13.
- Buckinx F, Gouspillou G, Carvalho LP, Marcangeli V, El Hajj Boutros G, Dulac M, et al. Effect of high-intensity interval training combined with l-citrulline supplementation on functional capacities and muscle function in dynapenic-obese older adults. Journal of clinical medicine. 2018;7(12):561.
- Sculthorpe NF, Herbert P, Grace F. One session of high-intensity interval training (HIIT) every 5 days, improves muscle power but not static balance in lifelong sedentary ageing men: a randomized controlled trial. Medicine. 2017;96(6).
- Thum JS, Parsons G, Whittle T, Astorino TA. High-intensity interval training elicits higher enjoyment than moderate intensity continuous exercise. PLoS One. 2017;12(1):662-71.
- Vella CA, Taylor K, Drummer D. High-intensity interval and moderate-intensity continuous training elicit similar enjoyment and adherence levels in overweight and obese adults. European journal of sport science. 2017;17(9):1203-1211.
- van de Poll MC, Soeters PB, Deutz NE, Fearon KC, Dejong CH. Renal metabolism of amino acids: its role in interorgan amino acid exchange. The American journal of clinical nutrition. 2004;79(2):185-197.
- Kim I-Y, Schutzler SE, Schrader A, Spencer HJ, Azhar G, Deutz NE, et al. Acute ingestion of citrulline stimulates nitric oxide synthesis but does not increase blood flow in healthy young and older adults with heart failure. American Journal of Physiology-Endocrinology and Metabolism. 2015;309(11):915-24.
- Moinard C, Maccario J, Walrand S, Lasserre V, Marc J, Boirie Y, et al. Arginine behaviour after arginine or citrulline administration in older subjects. British Journal of Nutrition. 2016;115(3):399-404.
- Moinard C, Le Plenier S, Noirez P, Morio B, Bonnefont-Rousselot D, Kharchi C, et al. Citrulline supplementation induces changes in body composition and limits age-related metabolic changes in healthy male rats. The Journal of nutrition. 2015;145(7):1429-1437.
- Faure C, Raynaud-Simon A, Ferry A, Daugé V, Cynober L, Aussel C, et al. Leucine and citrulline modulate muscle function in malnourished aged rats. Amino acids. 2012;42(4):1425-1433.
- Bouillanne O, Melchior J-C, Faure C, Paul M, Canouï-Poitrine F, Boirie Y, et al. Impact of 3-week citrulline supplementation on postprandial protein metabolism in malnourished older patients: The Ciproage randomized controlled trial. Clinical Nutrition. 2019;38(2):564-76.
- Herbert P, Grace FM, Sculthorpe NF. Exercising caution: prolonged recovery from a single session of high-intensity interval training in older men. J Am Geriatr Soc. 2015;63(4):817-818.
- Gonzales JU, Raymond A, Ashley J, Kim Y. Does l‐citrulline supplementation improve exercise blood flow in older adults? Experimental physiology. 2017;102(12):1661-1671.
- Coburn JW, Malek MH. NSCA's essentials of personal training: USA: Human Kinetics; 2012.
- Van Roie E, Delecluse C, Coudyzer W, Boonen S, Bautmans I. Strength training at high versus low external resistance in older adults: effects on muscle volume, muscle strength, and force–velocity characteristics. Experimental gerontology. 2013;48(11):1351-1361.
- Housh Dona J, Terry J, Joseph P, Loree L, Glen O. Anthropometric estimation of thigh muscle cross-sectional area. Med Sci Sport Exer 1995; 27: 784-91.
- Gm A. Exercise physiology laboratory manual. Tehran: Hatmi publisher. 2014.
- Karlsen T, Aamot I-L, Haykowsky M, Rognmo Ø. High intensity interval training for maximizing health outcomes. Progress in cardiovascular diseases. 2017;60(1):67-77.
- Hayes LD, Sculthorpe N, Herbert P, Baker JS, Spagna R, Grace FM. Six weeks of conditioning exercise increases total, but not free testosterone in lifelong sedentary aging men. The Aging Male. 2015;18(3):195-200.
- Papadia C, Osowska S, Cynober L, Forbes A. Citrulline in health and disease. Review on human studies. Clinical Nutrition. 2018;37(6):1823-1828.
- Figueroa A, Alvarez-Alvarado S, Ormsbee MJ, Madzima TA, Campbell JC, Wong A. Impact of L-citrulline supplementation and whole-body vibration training on arterial stiffness and leg muscle function in obese postmenopausal women with high blood pressure. Experimental gerontology. 2015;63:35-40.
- Besco R, Sureda A, Tur JA, Pons A. The effect of nitric-oxide-related supplements on human performance. Sports medicine. 2012;42(2):99-117.
- Hickner RC, Tanner CJ, Evans CA, Clark PD, Haddock A, Fortune C, et al. L-citrulline reduces time to exhaustion and insulin response to a graded exercise test. Medicine and science in sports and exercise. 2006;38(4):660-666.