Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

1 PhD Student of Exercise Physiology, Kish International Campus, University of Tehran, Kish, Iran

2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

3 Professor, Department of Physical Education, Tarbiat Modares University, Tehran, Iran

4 . Assistant Professor, Department of Sport Sciences, Kish International Campus, University of Tehran, Kish, Iran

5 Associate Professor, Department of Sport Sciences, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

Abstract

The aim of the present study was to investigate the effect of resistance training and blood flow occlusion on gene expression of signal transfer agent and activator of transcription (STAT3) and muscle-forming agent 5 (Myf5) in non-athlete active young subjects. 20 men (age range 25 ± 5 years) were randomly divided into two groups (ach group 10 subjects): eccentric resistance training with and without blood flow restriction (BFR). Using isokinetic set, eccentric resistance training protocol (ECC RET) including about 70 repetitions of knee extensor muscles was performed. Occlusion was created using pneumatic pressure device. After local anesthesia with lidocaine 1% and norepinephrine, blood samples were collected from active vastus lateralis 48 hours before and 24 hours after the training. STAT3 and MyF5 were measured by immunohistochemistry. Gene expression of STAT3 in BFR group in the posttest was 3.846 times higher than the pretest (P=0.001) which showed a significant difference. Gene expression of MyF5 in BFR group in the posttest was 3.479 times higher than the pretest (P=0.005) which showed a significant difference. Eccentric resistance training with blood flow occlusion may maintain and improve muscle mass by increasing the gene expression of STAT3 and MyF5.

Keywords

Main Subjects

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