The Effect of Two-month Concurrent Training with and without Calorie Restriction on Sirtuin-1 Activity and Content in the Peripheral Blood Mononuclear Cells of Inactive Men

Document Type : Research Paper


1 . Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran, b: Associate Professor of Exercise Physiology, Department of Biological Sciences in Sport,

2 . PhD Student, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran

3 . Associate Professor of Physiology, Department of Biology, Faculty of Science, Payame Noor University, Tehran, Iran

4 Associate Professor of Immunology, Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


Sirtuins have become prominent molecules in the aging process. Therefore, the present study was conducted to compare the effect of two months of concurrent training with and without calorie restriction on sirtuin-1 activity and content in the peripheral blood mononuclear cells (PBMCs) of inactive men. Therefore, thirty inactive men participated in three homogeneous groups: calorie restriction (CR: n=10), concurrent training (T: n=10), and concurrent training with calorie restriction (TCR: n=10). The calorie restriction was about -30 kcal•kg-1•week-1. The training consisted of five days a week (Resistance Training: two sessions / week-1, and High- Intensity Interval Training: three sessions / week-1). Sirtuin-1 content and activity in PBMCs were measured by ELISA and fluorometric assay, respectively. To measure oxidative stress, Plasma total antioxidant capacity (TAC) and serum malondialdehyde (MDA) levels were determined by FRAP and spectrophotometry, respectively. Data were analyzed using analysis of variance at a significance level ≤ 0.05. The results showed that Sirtuin-1 activity and TAC of all three groups were significantly increased after two-month interventions (p < 0.05). However, the changes in sirtuin-1 content and MDA levels were not significant in any groups (P> 0.05). Given the optimal changes in sirtuin-1 activity in PBMCs, TAC, and somebody composition components following two-months of concurrent training without calorie restriction, it can be concluded that concurrent training may be a more appropriate intervention for modulating the cell survival indicator in inactive men.


Main Subjects

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