Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


1 Department of Biological Sciences in Sport and Health, Faculty of Sports Sciences and Health, Shahid Beheshti University, Tehran, Iran

2 Department of Medical Basic Sciences, University of Social Welfare and Rehabilitation Sciences Tehran, Iran,

3 Department of Exercise Physiology, Sport Science Faculty, Kharazmi University, Tehran, Iran

4 Department of Exercise Physiology, Sport Science Faculty, Kharazmi University, Tehran, Iran,

5 School of Human Kinetics and Recreation, Memorial University of Newfoundland, St. John’s, NL, Canada


Nowadays, many studies have shown that exercise improves brain health and performance and may also increase neurological pathways in the brain. The aim of the present study was to investigate the effect of 8 weeks of high intensity interval training (HIIT) on Klotho, PLP and TNF-α concentrations in mice. Female mice were randomly divided to exercise (EX) or control (Con) groups. In the EX group, animals performed 8-week of HIIT. Mice initially ran at 6 m/min for 3 min at 0% grade, with the speed progressively increasing by 3 m/min every 3 min until exhaustion - when the mice were unable to maintain the required running speed. The maximal speed obtained was then used to calculate the individualized running speed for mice in the EX group. HIIT involved treadmill running 5 days per week for the final 6 weeks. Klotho, PLP and TNF-α expressions were studied using Western blot. Mice in the EX group had a significant increase in klotho and PLP concentrations compared to the control group (p≤0.01 and p≤0.05, respectively). Also, in the exercise groups mice had a significant decrease in TNF-α concentrations in compared to the control group (p≤0.05). Our data demonstrate that HIIT increased klotho and PLP and deceased neuroinflammatory cytokines. These proteins are associated with increasing myelination and protect the central nerve system against neurodegenerative diseases.


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