Preventive Effect six Weeks of Swimming Training on the Levels of Cytokines and Brain-Derived Neurotrophic Rat Model of EAE

Hoseini, Sayd  Mojtaba; Falah Mohammadi, Ziya; Talebi, Vahid

doi:10.22059/jsb.2019.246687.1223

Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

¹ PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran

² . Associate Professor PhD of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran

³ PhD student of Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran

https://doi.org/10.22059/jsb.2019.246687.1223

Abstract

Multiple sclerosis is a progressive and chronic autoimmune disease of the central nervous system that is associated with the destruction of the myelin sheath of nerve cells. The aim of this study was to investigate the preventive effect of six weeks of forced swimming on the levels of TNF-α, IL-10 and BDNF is the EAE model rats. Twenty-five female Lewis were divided into five groups: healthy control, MS control, healthy swimming, MS swimming, and solvent. Swimming training for 6 weeks and 5 days a week. Swimming training program began with 30 minutes daily five-minute period in the second week with an increase to 60 minutes. Overload training was set by adjusting the power and speed of the water while swimming. Also, EAE was induced model at the end of the sixth week of training and After 14 days’ mice were sacrificed. Right Cerebral hemisphere for the histology and right Cerebral hemisphere was transferred to the lab for analysis of protein measurements. Swimming exercise in rats EAE model leads to a significant reduction in TNF-α (P=0/001) and no significant change in IL-10 (P=0/71) and BDNF (P=0/70) was. In rats trained clinical signs appeared delayed. Furthermore, results of histological staining showed that certain differences were observed between trained and untrained. EAE-induced demyelination model increases the damage to brain tissue Shows that swimming exercise intensity and duration of six weeks can be effective in delaying symptoms, although these symptoms are delayed in the short term.

Keywords

References

1. Barnett MH, Prineas JW. Relapsing and remitting multiple sclerosis: pathology of the newly forming lesion. Annals of neurology. 2004;55(4):458-68.

2. Pedotti R, De Voss JJ, Steinman L, Galli SJ. Involvement of both ‘allergic’and ‘autoimmune’mechanisms in EAE, MS and other autoimmune diseases. Trends in immunology. 2003;24(9):479-84.

3. Mancini A, Gaetani L, Di Gregorio M, Tozzi A, Ghiglieri V, Calabresi P, et al. Hippocampal neuroplasticity and inflammation: relevance for multiple sclerosis. Multiple Sclerosis and Demyelinating Disorders. 2017;2(1):2.

4. Das UN. Anti-inflammatory nature of exercise. Nutrition. 2004;20(3):323.

5. Goldhammer E, Tanchilevitch A, Maor I, Beniamini Y, Rosenschein U, Sagiv M. Exercise training modulates cytokines activity in coronary heart disease patients. International journal of cardiology. 2005;100(1):93-9.

6. Cudrici C, Niculescu T, Niculescu F, Shin ML. Oligodendrocyte cell death in pathogenesis of multiple sclerosis: Protection of oligodendrocytes from apoptosis by complement. Journal of rehabilitation research and development. 2006;43(1):123.

7. Hohlfeld R. Neurotrophic cross-talk between the nervous and immune systems: relevance for repair strategies in multiple sclerosis? Journal of the neurological sciences. 2008;265(1):93-6.

8. Rossi S, Furlan R, De Chiara V, Musella A, Giudice TL, Mataluni G, et al. Exercise attenuates the clinical, synaptic and dendritic abnormalities of experimental autoimmune encephalomyelitis. Neurobiology of disease. 2009;36(1):51-9.

9. Bernardes D, Oliveira-Lima OC, da Silva TV, Faraco CCF, Leite HR, Juliano MA, et al. Differential brain and spinal cord cytokine and BDNF levels in experimental autoimmune encephalomyelitis are modulated by prior and regular exercise. Journal of neuroimmunology. 2013;264(1):24-34.

10. Castellano V, White LJ. Serum brain-derived neurotrophic factor response to aerobic exercise in multiple sclerosis. Journal of the neurological sciences. 2008;269(1):85-91.

11. Patel DI, White LJ. Effect of 10-day forced treadmill training on neurotrophic factors in experimental autoimmune encephalomyelitis. Applied Physiology, Nutrition, and Metabolism. 2013;38(2):194-199.

12. Basso DM, Hansen CN. Biological basis of exercise-based treatments: spinal cord injury. PM&R. 2011;3(6):S73-S7.

13. Mirdar Sh, Arab A, Hedayati M, Hajizade A. The effect of pregnant rat swimming on hypoxia-inducible factor-1α levels of neonatal lung. Tehran University Medical Journal TUMS Publications. 2012;69(12):754-60.

14. Yaghmaei P NM, Mahdavi M,Mehrvarz T, Zahra Nazari. The effect of different doses of bee venom serum levels of interleukin-6 in Lewis rats EAE (a model for studying multiple sclerosis). Pajoohandeh Journal. 2013: 18 (2): 69-75. [In Persian].

15. Bernardes D, Brambilla R, Bracchi‐Ricard V, Karmally S, Dellarole A, Carvalho‐Tavares J, et al. Prior regular exercise improves clinical outcome and reduces demyelination and axonal injury in experimental autoimmune encephalomyelitis. Journal of neurochemistry. 2016;136(S1):63-73.

16. Klaren RE, Stasula U, Steelman AJ, Hernandez J, Pence BD, Woods JA, et al. Effects of exercise in a relapsing‐remitting model of experimental autoimmune encephalomyelitis. Journal of Neuroscience Research. 2016;94(10):907-14.

17. Huang Y, Dreyfus CF. The role of growth factors as a therapeutic approach to demyelinating disease. Experimental neurology. 2016;283:531-540.

18. Kierkegaard M, Lundberg IE, Olsson T, Johansson S, Ygberg S, Opava C, et al. High-intensity resistance training in multiple sclerosis—An exploratory study of effects on immune markers in blood and cerebrospinal fluid, and on mood, fatigue, health-related quality of life, muscle strength, walking and cognition. Journal of the neurological sciences. 2016;362:251-7.

19. Zhu W, Frost EE, Begum F, Vora P, Au K, Gong Y, et al. The role of dorsal root ganglia activation and brain‐derived neurotrophic factor in multiple sclerosis. Journal of cellular and molecular medicine. 2012;16(8):1856-65.

20. Lim S-Y, Constantinescu CS. TNF-α: A paradigm of paradox and complexity in multiple sclerosis and its animal models. Open Autoimmun J. 2010;2:160-70.

21. Abtahi froushani, SM, Delerezh N, Hobbenaghi R, Mosayebi GH. The Effects of Atorvastatin on the Treatment of Experimental Autoimmune Encephalomyelitis and its Role in the Response of T-Helper Lymphocytes. Journal of Isfahan Medical School. 2012. 30(188);622-623. (in Persian).

22. Saraiva M, O'Garra A. The regulation of IL-10 production by immune cells. Nature Reviews Immunology. 2010;10(3):170-81.

23. Ang E, Wong P, Moochhala S, Ng Y. Cytokine changes in the horizontal diagonal band of Broca in the septum after running and stroke: a correlation to glial activation. Neuroscience. 2004;129(2):337-47.

Journal of Sport Biosciences

Preventive Effect six Weeks of Swimming Training on the Levels of Cytokines and Brain-Derived Neurotrophic Rat Model of EAE

References

References

Volume 12, Issue 1
June 2020
Pages 53-65

Preventive Effect six Weeks of Swimming Training on the Levels of Cytokines and Brain-Derived Neurotrophic Rat Model of EAE

References

References

Volume 12, Issue 1June 2020Pages 53-65

Volume 12, Issue 1
June 2020
Pages 53-65