A Comparison of the Effect of High Intensity Interval Training and School –Based Training on has-miR125a-5p, CRP and Lipid Profile of Overweight and Obese Adolescents

Valipoor, Sadegh; Minasian, Vazgan; Hovsepian, Silva

doi:10.22059/jsb.2020.293458.1369

Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

¹ PhD Student, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran

² Associate Professor, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran

³ Assistant Professor, Child Growth and Development Research Center, Imam Hossein Children's Hospital, Isfahan, Iran

https://doi.org/10.22059/jsb.2020.293458.1369

Abstract

Endothelial dysfunction is an early indicator of cardiac diseases, and loss of normal homeostatic function in the blood vessels. The aim of this study was to compare the effect of school-based and high intensity interval training on miR125a-5p, C-reactive protein and lipid profile of overweight and obese adolescents. 45 overweight and obese male students (mean age 14±1 years, body mass index 26.43±2.17 kg/m²) were voluntarily divided into three equal groups: school-based training, high intensity interval training and control. Subjects participated in the training for12 weeks and 3 sessions per week. Changes of circulating miR125a-5p, C-reactive protein and lipid profile were measured before and after the training interventions. Findings revealed that C-reactive protein, body mass index, WHR, body fat percentage, low-density lipoprotein, and triglyceride of experimental subjects decreased compared with the control group (P≤0.05). There were significant increases in the VO₂peak and high density lipoprotein levels of subjects (P=0.001), but the differences between experimental groups were not significant in both variables (P=0.15). Also, there was a significant increase in the levels of miR125a-5p, and the difference between the experimental groups was significant (P=0.001). Generally, it seems that both types of training improved the endothelial function and lipid profile, but school-based training is relatively more effective in adolescents. Therefore, based on the difficult nature of high intensity interval training for obese children, further use of school-based training is suggested.

Keywords

Main Subjects

Exercise Physiology

References

1. Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell. 2009;136(2):215-33.

2. Wang S, Liao J, Huang J, Yin H, Yang W, Hu M. miR-214 and miR-126 were associated with restoration of endothelial function in obesity after exercise and dietary intervention. Journal of Applied Biomedicine. 2018;16(1):34-9.

3. Gozal D, Kheirandish-Gozal L. Cardiovascular morbidity in obstructive sleep apnea: oxidative stress, inflammation, and much more. Am J Respir Crit Care Med. 2008;177(4):369-75.

4. Khalyfa A, Kheirandish-Gozal L, Bhattacharjee R, Khalyfa AA, Gozal D. Circulating microRNAs as Potential Biomarkers of Endothelial Dysfunction in Obese Children. Chest. 2016;149(3):786-800.

5. Ribeiro MM, Silva AG, Santos NS, Guazzelle I, Matos LN, Trombetta IC, et al. Diet and exercise training restore blood pressure and vasodilatory responses during physiological maneuvers in obese children. Circulation. 2005;111(15):1915-23.

6. Davignon J, Ganz P. Role of endothelial dysfunction in atherosclerosis. Circulation. 2004;109(23 Suppl 1):27-32.

7. Hirata Y, Nagata D, Suzuki E, Nishimatsu H, Suzuki J, Nagai R. Diagnosis and treatment of endothelial dysfunction in cardiovascular disease. Int Heart J. 2010;51(1):1-6.

8. Gunji T, Matsuhashi N, Sato H, Fujibayashi K, Okumura M, Sasabe N, et al. Helicobacter pylori infection is significantly associated with metabolic syndrome in the Japanese population. Am J Gastroenterol. 2008;103(12):3005-10.

9. Akinci G, Akinci B, Coskun S, Bayindir P, Hekimsoy Z, Ozmen B. Evaluation of markers of inflammation, insulin resistance and endothelial dysfunction in children at risk for overweight. Hormones (Athens). 2008;7(2):156-62.

10. Blake GJ, Ridker PM. Novel clinical markers of vascular wall inflammation. Circ Res. 2001;89(9):763-71.

11. Pedersen BK. The anti-inflammatory effect of exercise: its role in diabetes and cardiovascular disease control. Essays Biochem. 2006;42:105-17.

12. Timpson NJ, Nordestgaard BG, Harbord RM, Zacho J, Frayling TM, Tybjaerg-Hansen A, et al. C-reactive protein levels and body mass index: elucidating direction of causation through reciprocal Mendelian randomization. Int J Obes (Lond). 2011;35(2):300-8.

13. Horak M, Zlamal F, Iliev R, Kucera J, Cacek J, Svobodova L, et al. Exercise-induced circulating microRNA changes in athletes in various training scenarios. 2018;13(1):1-8.

14. Adamo KB, Langlois KA, Brett KE, Colley RC. Young children and parental physical activity levels: findings from the Canadian health measures survey. Am J Prev Med. 2012;43(2):168-75.

15. Stenevi-Lundgren S, Daly RM, Karlsson MK. A school-based exercise intervention program increases muscle strength in prepubertal boys. Int J Pediatr. 2010; 30 (70); 63-65.

16. Lu KD, Cooper DM, Haddad F, Radom-Aizik S. Four Months of a School-Based Exercise Program Improved Aerobic Fitness and Clinical Outcomes in a Low-SES Population of Normal Weight and Overweight/Obese Children With Asthma. Front Pediatr. 2018;6:380-3.

17. MacKelvie KJ, Khan KM, Petit MA, Janssen PA, McKay HA. A school-based exercise intervention elicits substantial bone health benefits: a 2-year randomized controlled trial in girls. Pediatrics. 2003;112(6 Pt 1):e447.

18. Gibala MJ, Little JP, Macdonald MJ, Hawley JA. Physiological adaptations to low-volume, high-intensity interval training in health and disease. J Physiol. 2012;590(5):1077-84.

19. Martinez JE, Taipeiro EF, Chies AB. Effects of Continuous and Accumulated Exercise on Endothelial Function in Rat Aorta. Arq Bras Cardiol. 2017;108(4):315-22.

20. Racil G, Ben Ounis O, Hammouda O, Kallel A, Zouhal H, Chamari K, et al. Effects of high vs. moderate exercise intensity during interval training on lipids and adiponectin levels in obese young females. Eur J Appl Physiol. 2013;113(10):2531-40.

21. Jackson AS, Pollock ML. Practical Assessment of Body Composition. Phys Sportsmed. 1985;13(5):76-90.

22. Matsuzaka A, Takahashi Y, Yamazoe M, Kumakura N, Ikeda A, Wilk B, et al. Validity of the Multistage 20-M Shuttle-Run Test for Japanese Children, Adolescents, and Adults. 2004;16(2):113-15.

23. Martin H, Filip Z, Robert I, Jan K, Jan C. Exercise-induced circulating microRNA changes in athletes in various training scenarios. PLoS One. 2018;13 (1); 1-14.

24. Parr E, Camera D, Burke L, Phillips S, Coffey V, Hawley J. Circulating microRNA responses between ‘high’ and ‘low’ responder’s responders to a 16-wk diet and exercise weight loss intervention. Plos One. 2016;11(4); 1-14.

25. Gomes G, Fernandes T, Soci U, Silveira A, Barretti D, Negrão C, et al. Obesity Downregulates MicroRNA-126 Inducing Capillary Rarefaction in Skeletal Muscle: Effects of Aerobic Exercise Training. Oxidative medicine and cellular longevity. 2017; (5):1-9.

24. Pena AS, Wiltshire E, MacKenzie K, Gent R, Piotto L, Hirte C, et al. Vascular endothelial and smooth muscle function relates to body mass index and glucose in obese and nonobese children. J Clin Endocrinol Metab. 2006;91(11):4467-71.

25. Koopman LP, McCrindle BW, Slorach C, Chahal N, Hui W, Sarkola T, et al. Interaction between myocardial and vascular changes in obese children: a pilot study. J Am Soc Echocardiogr. 2012;25(4):401-10.

26. Tryggestad JB, Thompson DM, Copeland KC, Short KR. Obese children have higher arterial elasticity without a difference in endothelial function: the role of body composition. Obesity (Silver Spring). 2012;20(1):165-71.

27. Lee YH, Song YW, Kim HS, Lee SY, Jeong HS, Suh SH, et al. The effects of an exercise program on anthropometric, metabolic, and cardiovascular parameters in obese children. Korean Circ J. 2010;40(4):179-84.

28. Plaisance EP, Grandjean PW. Physical activity and high-sensitivity C-reactive protein. Sports Med. 2006;36(5):443-58.

29. Lambers S, Van Laethem C, Van Acker K, Calders P. Influence of combined exercise training on indices of obesity, diabetes and cardiovascular risk in type 2 diabetes patients. Clin Rehabil. 2008;22(6):483-92.

30. Cauza E, Hanusch-Enserer U, Strasser B, Ludvik B, Metz-Schimmerl S, Pacini G, et al. The relative benefits of endurance and strength training on the metabolic factors and muscle function of people with type 2 diabetes mellitus. Arch Phys Med Rehabil. 2005;86(8):1527-33.

31. Nicklas BJ, Hsu FC, Brinkley TJ, Church T, Goodpaster BH, Kritchevsky SB, et al. Exercise training and plasma C-reactive protein and interleukin-6 in elderly people. J Am Geriatr Soc. 2008;56(11):2045-52.

32. Raitakari OT, Juonala M, Kahonen M, Taittonen L, Laitinen T, Maki-Torkko N, et al. Cardiovascular risk factors in childhood and carotid artery intima-media thickness in adulthood: the Cardiovascular Risk in Young Finns Study. Jama. 2003;290(17):2277-83.

33. Shakiba M, Fathi M, Gholami Avval S. The effect of eight weeks of continuous and interval training on serum TNF-ɑ, IL-6 and hs-CRP levels in female. Journal of Practical Studies of Biosciences in Sport (JPSBS). 2018;6(12):71-81[In persian].

34. Hallmark R, Patrie JT, Liu Z, Gaesser GA, Barrett EJ, Weltman A. The effect of exercise intensity on endothelial function in physically inactive lean and obese adults. PLoS One. 2014;9(1):85-6.

35. Church TS, Barlow CE, Earnest CP, Kampert JB, Priest EL, Blair SN. Associations between cardiorespiratory fitness and C-reactive protein in men. Arterioscler Thromb Vasc Biol. 2002;22(11):1869-76.

Journal of Sport Biosciences

A Comparison of the Effect of High Intensity Interval Training and School –Based Training on has-miR125a-5p, CRP and Lipid Profile of Overweight and Obese Adolescents

References

References

Volume 12, Issue 2
September 2020
Pages 173-187

A Comparison of the Effect of High Intensity Interval Training and School –Based Training on has-miR125a-5p, CRP and Lipid Profile of Overweight and Obese Adolescents

References

References

Volume 12, Issue 2September 2020Pages 173-187

Volume 12, Issue 2
September 2020
Pages 173-187