Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

1 Ph.D. Student, Department of Physical Education and Sport Sciences, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran

2 Associate Professor, Department of Physical Education and Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran

3 . Assistant Professor, Department of Physical Education and Sport Sciences, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran

4 Assistant Professor, Department of Physical Education and Sport Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Abstract

The aim of the present study was to examine the effect of concurrent training order on some factors of physical fitness, functional capacity and serum levels of Myostatin and Follistatin in postmenopausal women. Methodology was clinical trial. 33 postmenopausal women (aged between 50 and 60) were randomly assigned to three groups: aerobic-resistance training (n=11), resistance-aerobic training (n=10) and control (n=12). Training interventions were performed for 8 weeks, 4 sessions per week. ANOVA with repeated measures was used to analyze the data.The results showed no significant differences in BMI, myostatin and follistatin concentrations among the three groups (P>0.05). The percentage of reduction in BMI was higher in A+R group and the percentage of reduction in myostatin and follistatin was higher in R+A group but this reduction was not significant. There was a significant difference between training and control groups in upper body muscular strength and endurance (P>0.05). There was a significant increase in upper body muscular strength and endurance in both training groups (P>0.05). The percentage of changes in the upper body muscular strength and endurance was higher in R+A group but this percentage was not significant. Based on these results, it can be stated that there were no significant differences between the different orders of concurrent training in the mentioned variables and postmenopausal women can use both training orders in order to increase upper body strength and endurance and prevent to increase myostatin and muscle atrophy.

Keywords

Main Subjects

1.   Garcia M, Mulvagh SL, Bairey Merz CN, Buring JE, Manson JE. Cardiovascular disease in women: clinical perspectives. Circulation research. 2016; 118(8):1273-93.
2.   Douchi T, Iemura A, Matsuo T, Kuwahata T, Oki T, Yoshimitsu N, et al. Relationship of head lean mass to regional bone mineral density in elderly postmenopausal women. Maturitas. 2003; 46(3):225-30.
3.   Kraemer WJ, Ratamess NA, French DN. Resistance training for health and performance. Current sports medicine reports. 2002; 1(3):165-71.
4.   Hittel DS, Axelson M, Sarna N, Shearer J, Huffman KM, Kraus WE. Myostatin decreases with aerobic exercise and associates with insulin resistance. Medicine and science in sports and exercise. 2010; 42(11):2023.
5.   Wood RH, Reyes R, Welsch MA, Favaloro-Sabatier J, Sabatier M, Lee CM, et al. Concurrent cardiovascular and resistance training in healthy older adults. Medicine & Science in Sports & Exercise. 2001; 33(10):1751-8.
6.   Hass CJ, Feigenbaum MS, Franklin BA. Prescription of resistance training for healthy populations. Sports medicine. 2001; 31(14):953-64.
7.   Organization WH. Research on the menopause in the 1990s: report of a WHO scientific group. 1996.
8.   Pedersen PK, JØrgensen K. Maximal oxygen uptake in young women with training, inactivity, and retraining. Medicine and science in sports. 1978; 10(4):233-7.
9.   Karavirta L, Tulppo MP, Laaksonen DE, Nyman K, Laukkanen RT, Kinnunen H, et al. Heart rate dynamics after combined endurance and strength training in older men. Medicine and science in sports and exercise. 2009; 41(7):1436-43.
10. Kraemer WJ, Patton JF, Gordon SE, Harman EA, Deschenes MR, Reynolds K, et al. Compatibility of high-intensity strength and endurance training on hormonal and skeletal muscle adaptations. Journal of applied physiology. 1995; 78(3): 976-89.
11. Bell G, Syrotuik D, Martin T, Burnham R, Quinney H. Effect of concurrent strength and endurance training on skeletal muscle properties and hormone concentrations in humans. European journal of applied physiology. 2000; 81(5):418-27.
12. Asad M, Vakili J. Effect of 8 weeks resistance training on myostatin serum level in overweight nonathletic women. Int J Sport Stud. 2013; 3:774-8.
13. Konopka AR, Douglass MD, Kaminsky LA, Jemiolo B, Trappe TA, Trappe S, et al. Molecular adaptations to aerobic exercise training in skeletal muscle of older women. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences. 2010; 65(11):1201-7.
14. Raue U, Slivka D, Minchev K, Trappe S. Improvements in whole muscle and myocellular function are limited with high-intensity resistance training in octogenarian women. Journal of applied physiology. 2009; 106(5):1611-7.
15. Zimmers TA, Davies MV, Koniaris LG, Haynes P, Esquela AF, Tomkinson KN, et al. Induction of cachexia in mice by systemically administered myostatin. Science. 2002; 296(5572):1486-8.
16. Elliott B, Shinwari Z, Altayar Z, Barrios L, Chaudhary G, Hanifa E, et al. editors. Circulating myostatin is reduced with aging in humans but not altered by short-term, high intensity training. Proceedings of the Physiological Society; 2017: The Physiological Society.
17. Dieli-Conwright CM, Spektor TM, Rice JC, Sattler FR, Schroeder ET. Influence of hormone replacement therapy on eccentric exercise induced myogenic gene expression in postmenopausal women. Journal of applied physiology. 2009; 107(5):1381-8.
18. Hansen J, Brandt C, Nielsen AR, Hojman P, Whitham M, Febbraio MA, et al. Exercise induces a marked increase in plasma follistatin: evidence that follistatin is a contraction-induced hepatokine. Endocrinology. 2011; 152(1):164-71.
18. Ivey FM, Roth SM, Ferrell RE, Tracy BL, Lemmer JT, Hurlbut DE, et al. Effects of age, gender, and myostatin genotype on the hypertrophic response to heavy resistance strength training. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2000; 55(11):M641-M8.
20. Lundberg TR, Fernandez-Gonzalo R, Gustafsson T, Tesch PA. Aerobic exercise alters skeletal muscle molecular responses to resistance exercise. Medicine & Science in Sports & Exercise. 2012; 44(9):1680-8.
21. Willoughby DS. Effects of heavy resistance training on myostatin mRNA and protein expression. Medicine and science in sports and exercise. 2004; 36(4):574-82.
22. Bird SP, Tarpenning KM, Marino FE. Designing resistance training programmes to enhance muscular fitness. Sports medicine. 2005; 35(10):841-51.
23. Murlasits Z, Kneffel Z, Thalib L. The physiological effects of concurrent strength and endurance training sequence: A systematic review and meta-analysis. Journal of sports sciences. 2018; 36(11):1212-9.
24. Hickson RC. Interference of strength development by simultaneously training for strength and endurance. European journal of applied physiology and occupational physiology. 1980; 45(2-3):255-63.
25. Anderson T, Kearney JT. Effects of three resistance training programs on muscular strength and absolute and relative endurance. Research Quarterly for Exercise and Sport. 1982; 53(1):1-7.
26. Brzycki M. Strength testing—predicting a one-rep max from reps-to-fatigue. Journal of Physical Education, Recreation & Dance. 1993; 64(1):88-90.
.27 Adams KJ, Swank AM, Barnard KL, Berning JM, Sevene-Adams PG. Safety of maximal power, strength, and endurance testing in older African American women. The Journal of Strength & Conditioning Research. 2000; 14(3):254-60.
28. Paulsen G, Myklestad D, Raastad T. The influence of volume of exercise on early adaptations to strength training. Journal of Strength and Conditioning Research. 2003; 17(1):115-20.
29. Medicine ACoS. American College of Sports Medicine position stand. Progression models in resistance training for healthy adults. Medicine and science in sports and exercise. 2009; 41(3):687.
30. Abe T, DeHoyos DV, Pollock ML, Garzarella L. Time course for strength and muscle thickness changes following upper and lower body resistance training in men and women. European journal of applied physiology. 2000; 81(3):174-80.
31. Cadore EL, Izquierdo M. Concurrent Training in Elderly. Concurrent Aerobic and Strength Training: Springer; 2019. p. 277-91.
32. Sheikholeslami-Vatani D, Siahkouhian M, Hakimi M, Ali-Mohammadi M. The effect of concurrent training order on hormonal responses and body composition in obese men. Science & Sports. 2015; 30(6):335-41.
33. Davitt PM, Pellegrino JK, Schanzer JR, Tjionas H, Arent SM. The effects of a combined resistance training and endurance exercise program in inactive college female subjects: Does order matter? The Journal of Strength & Conditioning Research. 2014; 28(7):1937-45.
34. Alves J, Saavedra F, Simão R, Novaes J, Rhea MR, Green D, et al. Does aerobic and strength exercise sequence in the same session affect the oxygen uptake during and postexercise? The Journal of Strength & Conditioning Research. 2012; 26(7):1872-8.
35. Dolezal BA, Potteiger JA. Concurrent resistance and endurance training influence basal metabolic rate in nondieting individuals. Journal of applied physiology. 1998; 85(2):695-700.
36. Staron R, Karapondo D, Kraemer W, Fry A, Gordon S, Falkel JE, et al. Skeletal muscle adaptations during early phase of heavy-resistance training in men and women. Journal of applied physiology. 1994; 76(3):1247-55.
37. Carroll TJ, Abernethy PJ, Logan PA, Barber M, McEniery MT. Resistance training frequency: strength and myosin heavy chain responses to two and three bouts per week. European journal of applied physiology and occupational physiology. 1998; 78 (3):270-5.
38. Chtara M, Chaouachi A, Levin GT, Chaouachi M, Chamari K, Amri M, et al. Effect of concurrent endurance and circuit resistance training sequence on muscular strength and power development. The Journal of Strength & Conditioning Research. 2008; 22(4):1037-45.
39. Collins MA, Snow TK. Are adaptations to combined endurance and strength training affected by the sequence of training? Journal of sports sciences. 1993; 11 (6):485-91.
40. Eddens L, van Someren K, Howatson G. The role of intra-session exercise sequence in the interference effect: a systematic review with meta-analysis. Sports Medicine. 2018; 48(1):177-88.
41. de Souza EO, Tricoli V, Aoki MS, Roschel H, Brum PC, Bacurau AV, et al. Effects of concurrent strength and endurance training on genes related to myostatin signaling pathway and muscle fiber responses. The Journal of Strength & Conditioning Research. 2014; 28(11):3215-23.
42. Lundberg TR, Fernandez-Gonzalo R, Tesch PA. Exercise-induced AMPK activation does not interfere with muscle hypertrophy in response to resistance training in men. Journal of applied physiology. 2014; 116 (6):611-20.
43. Fyfe JJ, Bishop DJ, Stepto NK. Interference between concurrent resistance and endurance exercise: molecular bases and the role of individual training variables. Sports medicine. 2014; 44 (6):743-62.
44. Schoenfeld B. Science and Development of Muscle Hypertrophy: Human Kinetics; 2016.
45. Nader GA. Concurrent strength and endurance training: from molecules to man. Medicine and science in sports and exercise. 2006; 38 (11):1965.
46. Carruthers NJ, Stemmer PM. Methionine oxidation in the calmodulin-binding domain of calcineurin disrupts calmodulin binding and calcineurin activation. Biochemistry. 2008; 47 (10):3085-95.
47. Murach KA, Bagley JR. Skeletal muscle hypertrophy with concurrent exercise training: contrary evidence for an interference effect. Sports medicine. 2016; 46 (8):1029-39.
48. Elliott BT, Herbert P, Sculthorpe N, Grace FM, Stratton D, Hayes LD. Lifelong exercise, but not short‐term high‐intensity interval training, increases GDF 11, a marker of successful aging: a preliminary investigation. Physiological reports. 2017; 5(13):e13343.
49. Biglari S, Gaeini AA, Kordi MR, GhardashiAfousi A. The effect of 8 weeks high-intensity interval training on myostatin and follistatin gene expression in gastrocnemius muscle of the rats. Journal of Arak University of Medical Sciences. 2018; 21(1):1-10.
50. Jensky NE, Sims JK, Dieli-Conwright CM, Sattler FR, Rice JC, Schroeder ET. Exercise does not influence myostatin and follistatin mRNA expression in young women. Journal of strength and conditioning research/National Strength & Conditioning Association. 2010; 24(2):522.
51. Vamvini MT, Aronis KN, Chamberland JP, Mantzoros CS. Energy deprivation alters in a leptin-and cortisol-independent manner circulating levels of activin A and follistatin but not myostatin in healthy males. The Journal of Clinical Endocrinology & Metabolism. 2011, 96(11):3416–3423
52. Crist BL, Alekel DL, Ritland LM, Hanson LN, Genschel U, Reddy MB. Association of oxidative stress, iron, and centralized fat mass in healthy postmenopausal women. Journal of Women's Health. 2009; 18(6):795-801.
53. Wisløff U, Støylen A, Loennechen JP, Bruvold M, Rognmo Ø, Haram PM, et al. Superior cardiovascular effect of aerobic interval training versus moderate continuous training in heart failure patients: a randomized study. Circulation. 2007; 115 (24):3086-94.
54. Attipoe S, Park J-Y, Fenty N, Phares D, Brown M. Oxidative stress levels are reduced in postmenopausal women with exercise training regardless of hormone replacement therapy status. Journal of women & aging. 2008; 20 (1-2):31-45.