The effect of eccentric exercise induced muscle damage at different times on Mitsogomin53 and Dysferlin levels in rat Soleus muscle

Document Type : Research Paper


1 PhD Student, Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran

2 Associate Professor, Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran

3 Assistant Professor, Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran

4 . Associate Professor, Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


Eccentric contraction during exercise causes muscle and sarcolemma damage. Mitsogomin53 and Dysferlin are the major sarcolemma repair proteins. Therefore, the aim of the present study was to investigate the effect of eccentric exercise-induced muscle damage at different times on skeletal muscle Mitsogomin53 and Dysferlin. Fifty male Wistar rats, after familiarization with the environment and the treadmill were divided into five control groups and five eccentric exercise groups. Soleus muscle in sterile conditions disassociate half, 24, 48, 72 and 168 hours (one week) after eccentric exercise (90 min interval running with 16 meter/min and -16 degree slope). Hematoxylin-eosin and western blotting methods were used to measure the number of inflammatory cells and extract the proteins of Mitsogomin53 and Dysferlin. Two-way ANOVA and independent t-test were used to compare the means with the significance level of p≤0.05. The number of inflammatory cells, Mitsogomin53 and Dysferlin proteins at the half, 24, 48, 72 and 168 hours (one week) after eccentric exercise in the exercise group were significantly higher than the control group (p


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