The Effect of Eight Weeks Circuit Resistance Training on the Levels of Interleukin-15, IGF-I and IGFBP-3 in Overweight Males

Document Type : Research Paper


1 MSc, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Assistant Professor, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran


Interleukin-15 (IL-15) is an important cytokine that secreted from skeletal muscles in response to exercise and has a different effect on various tissue such as muscular tissue. Present study conducted aimed to investigate the effect of eight weeks of circuit resistance training on serum levels of IL-15 in young overweight males. 20 overweight young males (BMI: 26.32±0.98 kg.m2) randomly divided into two control and circuit resistance training groups (n=10). Circuit resistance training program performed for eight weeks and three session per week. Each training session consisted of nine exercise that performed with 50 to 60 percent of 1RM. Over this time, the subjects in control group continue their routine daily program. After 48 hours from the last training session, post-test blood samples taken up to 7 ml from brachial vein, as in pre-test, in order to measurement the levels of desired variables including IL-15, IGF-I and IGFBP-3. Present study findings indicated that changes in the levels of IL-15, IGF-I and IGFBP-3 were not statistically significant between the control and circuit resistance training groups (p>0.05). However, percent body fat in trained group indicated significant decrease compared to control group (p=0.003). According to present study findings, it seems that circuit resistance training positive effects over eight weeks in overweight young males is mediated by other pathways beside the changes in levels of studied variables including IL-15.


1. Bonecchi R, Garlanda C, Mantovani A, Riva F. Cytokine decoy and scavenger receptors as key regulators of immunity and inflammation. Cytokine. 2016;87:37-45.
2. Heled Y, Fleischmann C, Epstein Y. Cytokines and their role in hyperthermia and heat stroke. Journal of basic and clinical physiology and pharmacology. 2013;24(2):85-96.
3. Ye J. Beneficial metabolic activities of inflammatory cytokine interleukin 15 in obesity and type 2 diabetes. Frontiers of medicine. 2015;9(2):139-45.
4. Patidar M, Yadav N, Dalai SK. Interleukin 15: A key cytokine for immunotherapy. Cytokine & growth factor reviews. 2016;31:49-59.
5. Argilés JM, López-Soriano FJ, Busquets S. Therapeutic potential of interleukin-15: a myokine involved in muscle wasting and adiposity. Drug discovery today. 2009;14(3-4):208-13.
6. Schnyder S, Handschin C. Skeletal muscle as an endocrine organ: PGC-1α, myokines and exercise. Bone. 2015;80:115-25.
7. Nadeau L, Aguer C. Interleukin-15 as a myokine: mechanistic insight into its effect on skeletal muscle metabolism. Applied Physiology, Nutrition, and Metabolism. 2018;44(3):229-38.
8. Duan Y, Li F, Wang W, Guo Q, Wen C, Li Y, et al. Interleukin‐15 in obesity and metabolic dysfunction: current understanding and future perspectives. Obesity reviews. 2017;18(10):1147-58.
9. Liu Z, Liang G, Gui L, Li Y, Liu M, Zhang X, et al. Weakened IL-15 production and impaired mTOR activation alter dendritic epidermal T cell homeostasis in diabetic mice. Scientific reports. 2017;7(1):6028.
10. Wang Y, Bai Y, Li Y, Liang G, Jiang Y, Liu Z, et al. IL-15 enhances activation and IGF-1 production of dendritic epidermal T cells to promote wound healing in diabetic mice. Frontiers in immunology. 2017;8:1557.
11. Quinn LS, Anderson BG, Drivdahl RH, Alvarez B, Argilés JM. Overexpression of interleukin-15 induces skeletal muscle hypertrophy in vitro: implications for treatment of muscle wasting disorders. Experimental cell research. 2002;280(1):55-63.
12. Nielsen AR, Mounier R, Plomgaard P, Mortensen OH, Penkowa M, Speerschneider T, et al. Expression of interleukin‐15 in human skeletal muscle–effect of exercise and muscle fibre type composition. The Journal of physiology. 2007;584(1):305-12.
در ... 445 IGFBP- و 3 IGF-I ، تأثیر هشت هفته تمرین مقاومتی دایرهای بر سطوح اینترلوکین 15
13. Tamura Y, Watanabe K, Kantani T, Hayashi J, Ishida N, Kaneki M. Upregulation of circulating IL-15 by treadmill running in healthy individuals: is IL-15 an endocrine mediator of the beneficial effects of endurance exercise? Endocrine journal. 2011;58(3):211-5.
14. Shamsi MM, Hassan ZM, Quinn LS, Gharakhanlou R, Baghersad L, Mahdavi M. Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype. Endocrine. 2015;49(2):396-403.
15. Kumaravel S, Hema R, Kamaleshwari A, Yusuf AD, Abu M, Nasir AN, et al. Effect of oven drying on the nutritional properties of whole egg and its components. Int J Food Sci Nutr. 2011;1(1):4-12.
16. Christiansen T, Paulsen SK, Bruun JM, Pedersen SB, Richelsen B. Exercise training versus diet-induced weight-loss on metabolic risk factors and inflammatory markers in obese subjects: a 12-week randomized intervention study. American Journal of Physiology-Endocrinology and Metabolism. 2010;298(4):E824-E31.
17. Rinnov A, Yfanti C, Nielsen S, Åkerström TC, Peijs L, Zankari A, et al. Endurance training enhances skeletal muscle interleukin-15 in human male subjects. Endocrine. 2014;45(2):271-8.
18. Romero-Arenas S, Blazevich AJ, Martínez-Pascual M, Pérez-Gómez J, Luque AJ, López-Román FJ, et al. Effects of high-resistance circuit training in an elderly population. Experimental gerontology. 2013;48(3):334-40.
19. Brentano MA, Cadore EL, Da Silva EM, Ambrosini AB, Coertjens M, Petkowicz R, et al. Physiological adaptations to strength and circuit training in postmenopausal women with bone loss. The Journal of Strength & Conditioning Research. 2008;22(6):1816-25.
20. Ghanbari-Niaki A, Nabatchian S, Hedayati M. Plasma agouti-related protein (AGRP), growth hormone, insulin responses to a single circuit-resistance exercise in male college students. Peptides. 2007;28(5):1035-9.
21. Ghanbari-Niaki A, Saghebjoo M, Hedayati M. A single session of circuit-resistance exercise effects on human peripheral blood lymphocyte ABCA1 expression and plasma HDL-C level. Regulatory peptides. 2011;166(1-3):42-7.
22. Ghanbari-Niaki A, Saeidi A, Ahmadian M, Gharahcholo L, Naghavi N, Fazelzadeh M, et al. The combination of exercise training and Zataria multiflora supplementation increase serum irisin levels in postmenopausal women. Integrative medicine research. 2018;7(1):44-52.
23. NAKHZAREE KJ, Mogharnasi M, HAGHIGHI A. Investigating the Response and Adaptation of Interleukin-15 to Resistance Training of Untrained Young Men. 2011.
24. Prestes J, Shiguemoto G, Botero JP, Frollini A, Dias R, Leite R, et al. Effects of resistance training on resistin, leptin, cytokines, and muscle force in elderly post-menopausal women. Journal of sports sciences. 2009;27(14):1607-15.
25. Pérez‐López A, McKendry J, Martin‐Rincon M, Morales‐Alamo D, Pérez‐Köhler B, Valadés D, et al. Skeletal muscle IL‐15/IL‐15Rα and myofibrillar protein synthesis after
446 علوم زیستی ورزشی، دورة 11 ، شمارة 4، زمستان 1398
resistance exercise. Scandinavian journal of medicine & science in sports. 2018;28(1):116-25.
26. Bazgir B, Salesi M, Koushki M, Amirghofran Z. Effects of eccentric and concentric emphasized resistance exercise on IL-15 serum levels and its relation to inflammatory markers in athletes and non-athletes. Asian journal of sports medicine. 2015;6(3).
27. Riechman SE, Balasekaran G, Roth SM, Ferrell RE. Association of interleukin-15 protein and interleukin-15 receptor genetic variation with resistance exercise training responses. Journal of Applied Physiology. 2004;97(6):2214-9.
28. Kim H-J, Park JY, Oh SL, Kim Y-A, So B, Seong JK, et al. Effect of treadmill exercise on interleukin-15 expression and glucose tolerance in zucker diabetic fatty rats. Diabetes & metabolism journal. 2013;37(5):358-64.
29. Nishida Y, Matsubara T, Tobina T, Shindo M, Tokuyama K, Tanaka K, et al. Effect of low-intensity aerobic exercise on insulin-like growth factor-I and insulin-like growth factor-binding proteins in healthy men. International journal of endocrinology. 2010;2010.
30. Schmitz KH, Ahmed RL, Yee D. Effects of a 9-month strength training intervention on insulin, insulin-like growth factor (IGF)-I, IGF-binding protein (IGFBP)-1, and IGFBP-3 in 30–50-year-old women. Cancer Epidemiology and Prevention Biomarkers. 2002;11(12):1597-604.
31. Krolopp JE, Thornton SM, Abbott MJ. IL-15 activates the Jak3/STAT3 signaling pathway to mediate glucose uptake in skeletal muscle cells. Frontiers in physiology. 2016;7:626.
32. Goossens GH. The role of adipose tissue dysfunction in the pathogenesis of obesity-related insulin resistance. Physiology & behavior. 2008;94(2):206-18.