Document Type : Research Paper I Open Access I Released under CC BY 4.0 license

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Abstract

 
The aim of this study was to investigate the effect of 10 weeks of resistance training on serum levels of heat shock protein 70 (HSP70) and insulin resistance in type 2 diabetic women. 20 women with type 2 diabetes were selected by purposive sampling method and randomly divided into experimental group (n = 10, mean age 47.13±6.27 year, BMI 28.45±3.32 kg/m²) and control group (n = 10, mean age 50.30±9.97 year, BMI 28.78±2.45 kg/m²). The training program consisted of 10 weeks of resistance training, 3 sessions per week for 90 minutes at 8 stations with 30-80% one repetition maximum (1RM). After 12 hours of fasting, blood samples were collected 48 hours before the study and 48 hours after the last training session. Data were analyzed using theShapiro Wilk test and independent and dependent t tests with SPSS version 21 at significance level (α<0.05). The results showed that 10 weeks of resistance training in experimental group significantly decreased HSP70 levels, glucose and insulin resistance (P<0.05) whereas insulin levels did not change significantly (P>0.05). HSP70 and glucose changes from pretest to the posttest in the experimental group were significant compared with the control group (P<0.05). It seems that resistance training reduces fasting blood glucose and insulin resistance and increases levels of HSP70 in patients with type 2 diabetes; therefore, this training can be recommended as an adjuvant therapy in these patients.

Keywords

  1. حقیقی، امیرحسین؛ یاراحمدی، هادی؛ شجاعی، ملیحه (1392). تأثیر 9 هفته تمرین هوازی بر سطح ویسفاتین سرم و شاخص مقاومت به انسولین در زنان چاق. مجلۀ علوم پزشکی سبزوار، 20(5)، ص 634-623.
  2. عصارزاده نوش‌آبادی، محسن؛ عابدی، بهرام (1392). اثرات تمرین ترکیبی بر شاخص مقاومت به انسولین و برخی نشانگرهای التهابی در مردان غیرفعال. فصلنامۀ افق دانش. 18(3)، ص 105-95.
  3. معظمی، مهتاب؛ بیژه، ناهید؛ عباسیان، صادق (1392). مقایسۀ اثر روزه‌داری ماه رمضان و فعالیت ورزشی هوازی منظم بر سطح سرمی پروتئین شوک حرارتی 70 کیلودالتونی (70HSP)، پروفایل لیپیدی و مقاومت به انسولینی افراد چاق غیرفعال. مجلۀ غدد درون‌ریز و متابولیسم ایران. 15(1)، ص 77-67.
    1. Akbarpour M. (1390). “The Effect of Resistance training on serum levels of Adipokine and Inflammatory Markers of cardiovascular disease in obese Men”. Qom Univ Med Sci J. 7(3):PP: 1-10.
    2. Bacchi E, Negri C, Elisabetta ZM, Milanese CH, Faccioli N, Trombetta M, et al. (2012). “Metabolic Effects of Aerobic Training and Resistance Training in Type 2 Diabetic Subjects”. Diabetes Care. 35(4):PP: 676-82.
    3. Bastard JP, Maachi M, Lagathu C, Kim MJ, Caron M, Vidal H, Capeau J, Feve B. (2006). “Recent advances in the relationship between obesity, inflammation, and insulin resistance”. Eur Cytokine Netw. 17(1):PP: 4-12.
    4. Brahm KT, Kanti BP, Abidi BA, Rizvi SI.(2013). “Markers of Oxidative Stress during Diabetes Mellitus”. Journal of Biomarkers, 8(1):PP: 28-38
    5. Danaei G, Finucane MM, Lu Y, Singh GM, Cowan MJ, Paciorek CJ, et al. (2011). "National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 207 million participants". Lancet. 378(9785):PP: 31-40.
    6. Dario P, Manfredi T, Rizzi A, Giovanni Gh, Carmine C. (2013). “Oxidative Stress in Diabetes: Implications for Vascular and Other Complications”. Int J Mol Sci. 14(11):PP: 21525–21550.

10. Earl G. Noble, Garry X. Shen. (2002). “Impact of Exercise and Metabolic Disorders on Heat Shock Proteins and Vascular Inflammation”. Autoimmune Diseases. 13(2):PP: 36-44.

11. Farah Nameni. (2012). “The Effect of a Single Bout Endurance Training on HSP70”. World Applied Sciences Journal. 19 (2): PP: 211-214.

12. Ford ES. (2002). “Does exercise reduce inflammation Physical activity and C-reactive protein among U.S. adults”. Epidemiology. 13(5):PP: 561-8.

13. Gielen S, Adams V, Möbius-Winkler S, Linke A, Erbs S, Yu J, Kempf W, Schubert A, Schuler G, Hambrecht R. (2003). “Anti-inflammatory effects of exercise training in the skeletal muscle of patients with chronic heart failure”. J Am Coll Cardiol. 42(5): PP: 861-8.

14. Hamilton CA1, Miller WH, Al-Benna S, Brosnan MJ, Drummond RD, McBride MW, et al. )2004.( " Strategies to reduce oxidative stress in cardiovascular disease". Clin Sci (Lond). 106(3):PP: 219-34.

15. Hooper PL, Hooper JJ. (2005). “Loss of defense against stress: diabetes and heat shock proteins”. Diabetes Technol Ther. 7(1):PP: 204-8.

16. Jackson AS, Pollock ML. (1978). “Generalized equations for predicting body density of men”. Br J Nutr. 40(3):PP: 497-504.

17. Kalmar B , Greensmith L. (2009). “Induction of heat shock proteins for protection against oxidative stress”. Advanced Drug Delivery Reviews. 61(4):pp: 310-318.

18. Kevin J. Milne , Earl G. Noble. (2002). “Exercise-induced elevation of HSP70 is intensity dependent”. Journal of Applied Physiology. 93(2):PP: 561-8.

19. Kiang JG, Tsokos GC. (1998). “Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology”. Pharmacol Ther. 80(2):PP: 183-201.

20. Kishiko Ogawa, Kiyoshi Sanada, Shuichi Machida, Mitsuharu Okutsu, and Katsuhiko Suzuki.(2010). "Resistance Exercise Training-Induced Muscle Hypertrophy Was Associated with Reduction of Inflammatory Markers in Elderly Women". Mediators of Inflammation . 7(2):PP: 131-140.

21. Kregel KC. (2002). ” Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance”. J Appl Physiol. 92(5):PP: 2177-2186.

22. Marsh SA1, Coombes JS.(2005). “Exercise and the endothelial cell”. Int J Cardiol. 99(2):PP: 165-9.

23. Mathur N, Pedersen BK. (2008) “Exercise as a mean to control low-grade systemic inflammation”. Mediators Inflamm.. 9(2):PP: 130-139.

24. Matos MA, Ottone VD, Duarte TC, Sampaio PF, Costa KB, Fonseca CA, Neves MP, Schneider SM, Moseley P, Coimbra CC, et al. (2010). “Exercise reduces cellular stress related to skeletal muscle insulin resistance”. Cell Stress Chaperones. 19(2) :PP: 263-270.

25. Nakhjavani M, Morteza A, Khajeali L, Esteghamati A, Khalilzadeh O, Asgarani F, et al. (2010). “Increased serum HSP70 levels are associated with the duration of diabetes”. Cell Stress Chaperones. 15(6):PP: 959–964.

26. Njemini R, Demanet C, Mets T.(2004). “Inflammatory status as an important determinant of heat shock protein 70 serum concentrations during aging”. Biogerontology. 5(1):PP: 31-38.

27. Paulsen G, Hanssen KE, Rønnestad BR, Kvamme NH, Ugelstad I, Kadi F, Raastad T. (2012). “Strength training elevates HSP27, HSP70 and αB-crystallin levels in musculi vastus lateralis and trapezius”. Eur J Appl Physiol. 112(5):PP: 1773-82.

28. Poehlman ET, Dvorak RV, DeNino WF, Brochu M, Ades PA. (2000). “Effects of resistance training and endurance training on insulin sensitivity in nonobese, young women: a controlled randomized trial”. J Clin Endocrinol Metab. 85(7):PP: 2463-8.

29. Rajeev G, Abul FF, Sheelu S, Monil S. (2012). “Evaluation of TNF-α and IL-6 Levels in Obese and Non-obese Diabetics: Pre- and Postinsulin Effects”. N Am J Med Sci. Apr . 4(4): PP: 180–184.

30. Saremi A. (2011). “Sporting Exercises and diabetes Mellitus type 2:A Review on Evidences”. Journal of cell and Tissue(JCT). 2(3): PP: 171-181.

31. Shaibi GQ1, Cruz ML, Ball GD, Weigensberg MJ, Salem GJ, Crespo NC, Goran MI. (2006). “Effects of resistance training on insulin sensitivity in overweight Latino adolescent males”. Med Sci Sports Exerc. 38(7):PP: 1208-15.

32. Sheri R, Ronald J, Bo Fernhall, Judith G, Regensteiner, Bryan J, Blissmer, Richard R, Rubin, Lisa Chasan-Taber, Ann L, Albright, and Barry Braun. (2010). “Exercise and Type 2 Diabetes” . Diabetes Care. Dec; 33(12):PP: e147–e167.

33. Steinacker JM, Lormes W, Reissnecker S, Liu Y. (2006). “New aspects of the hormone and cytokine response to training”. Eur J Appl Physiol. 91:PP: 382–391.

34. Yabunaka N, Ohtsuka Y, Watanabe I, Noro H, Fujisawa H, Agishi Y. (1995). “Elevated levels of heat-shock protein 70 (HSP70) in the mononuclear cells of patients with non-insulin-dependent diabetes mellituss.” Diabetes Res Clin Pract. 30(2):PP: 143-7.