Document Type : Research Paper I Open Access I Released under CC BY 4.0 license



Brain derived neurotrophic factor (BDNF) plays an important role in neuronal growth transmission, plasticity and overall central nervous system health. For this reason, this study was conducted to investigate the independent and combined effects of 4 week omega3 supplementation with plant (Flax seed extract), and animal (fish oil) origin, with or without plyometric training on BDNF serum concentration in active men. Participants were 42 physical education students that randomly divided into six groups. The training and combination groups performed 4 weeks of selected plyometric training. Furthermore, supplementary and combination groups consumed 3000 mg omega3 daily for 4 weeks. Data were analyzed using one-way ANOVA and tukey post hoc. Level of significant was p


1. ابراهیم‌زاده عطاری، وحیده؛ پورقاسم، بهرام؛ رفرف، مریم؛ قربانی، ابوالفضل؛ طبیبی، هادی (1388). تأثیردانۀگیاهیبزرک برسطحسرمیپروفایللیپیدیو مالوندیآلدئیدخرگوش‌هایهیپرلیپیدمیک. مجلۀ علوم دارویی، (2)، ص 204- 195.
2. میرزایی، سعید؛ فلاح محمدی، ضیاء؛ حاجی‌زاده مقدم، اکبر؛ فتحی، رزیتا؛ علیزاده، رستم؛ رنجبر، روح‌الله (1390). " اثر 8 هفته تمرین استقامتی با مدت‌های مختلف بر سطوح فاکتورهای نوروتروفیک مشتق از مغز در پلاسمای موش‌های صحرایی نر" . پژوهش در علوم ورزشی، (10)، ص 128- 115.
3. Barde, YA.; Edgar, D.; Thoenen, H.(1982). “Purification of a new neurotrophic factor from mammalian brain”. EMBO J, 1, 549-553.  
4. Beattie, MS.; Harrington, AW.; Lee, R.; Kim, JY.; Boyce, SL.; Longo, FM.; et al. (2002). “ProNGF induces p75-mediated death of oligodendrocytes following spinal cord injury”. Neuron, 36, 375-386.
5- Cassilhas RC, Lee KS, Fernandes J, Oliveira MG, Tufik S, Meeusen R, de Mello MT(2012). “Spatial memory is improved by aerobic and resistance exercise through divergent molecular mechanisms”. Neuroscience. 202,309-17.
6. Chiaramello, S. Dalmasso, G. Bezin, L. Marcel, D. Jourdan, F. Peretto, P. et al. (2007). “BDNF/TrkB interaction regulates migration of SVZ precursor cells via PI3-K and MAP-K signalling pathways”. European Journal of Neuroscience, 26(7), 1780–1790.
7. Conklin SM, Gianaros PJ, Brown SM, Yao JK, Hariri AR, Manuck SB, Muldoon  MF. (2007). “Long-chain omega-3 fatty acid intake is associated positively with corticolimbic gray matter volume in healthy adults” , 29, (3),209-12.
8. Duman, R.S. (2002). “Synaptic plasticity and mood disorders”. Mol. Psychiatry, 7, 29–34.
9. Gates, MA.; Tai, CC.; Macklis, JD. (2000). “Neocortical neurons lacking the protein-tyrosine kinase B receptor display abnormal differentiation and process elongation in vitro and in vivo”. Neuroscience, 98, 437-447.
10- Goekint M, De Pauw K, Roelands B, Njemini R, Bautmans I, Mets T, Meeusen R. (2010). “Strength training does not inXuence serum brain-derived neurotrophic factor”. Eur J Appl Physiol. 110(2),285-93.
11. Gianotti Luca, Braga Marco, Fortis Claudio, Soldini Laura, et al. (1999). “A Prospective, Randomized Clinical Trial on Perioperative Feeding With an Arginine, Omega-3 Fatty Acid, and RNA-Enriched Enteral Diet: Effect on Host Response and Nutritional Status”. Journal of Parenteral and Enteral Nutrition; 23, 314-320.
12. GregM.C, Qiu-LanMa, SallyA.F. (2009). “Omega-3fattyacidsanddementia. Prostaglandins, Leukotrienes and Essential Fatty Acids”, 81, 213–221.
 13. Hofer, M.; Pagluisi, SR.; Hohn, A.; Leibrock, J.; Barde, Y. (1990). “Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain”. EMBO J, 9, 2459-2464.
14. Hui-Min Su. (2010). “Mechanisms of n-3 fatty acid-mediated development and maintenance of learning memory performance”, Journal of Nutritional Biochemistry 21, 364–373.
15. Janice K. Glaser, Martha A, Rebecca A, William B, Ronald G. (2011). “Omega-3 supplementation lowers inflammation and anxiety in medical students: A randomized controlled trial”. Brain, Behavior, and Immunity. 25, 1725–1734.
16. Karege, F. Schwald, M. Cisse, M. (2002). “Postnatal developmental profile of brain derived neurotrophic factor in rat brain and platelets”. Neurosci. Lett, 328 (3), 261–264.
17. Lang, U.E., Hellweg, R. Seifert, F. Schubert, F, & Gallinat, J. (2007). “Correlation between serum brain-derived neurotrophic factor level and an in vivo marker of cortical integrity”. Biological Psychiatry, 62(5), 530–535.
18. Ma, Y.L., Wang, H.L., Wu, H.C., Wei, C.L., Lee, E.H.Y. (1998). “Brain-derived neurotrophic factor antisense oligonucleotide impairs memory retention and inhibits long term potentiation in rats”. Neuroscience, 82, 957–967.
19. Matsuoka Y, Nishi D, Yonemoto N, Hamazaki K, Hamazaki T, Hashimoto K. (2010).Potential Role of Brain-Derived Neurotrophic Factor in Omega–3 Fatty Acid Supplementation to Prevent Posttraumatic Distress after Accidental Injury: An Open-Label Pilot Study”. Psychother Psychosom,80(5),310-312.
20. Matsuoka Y. (2011). “Clearance of fear memory from the hippocampus through neurogenesis by omega-3 fatty acids: a novel preventive strategy for posttraumatic stress disorder? “,Biopsychosoc. 8(3),425-431.
21. Mizuno, M. Yamada, K. Olariu, A. Nawa, H. Nabeshima, T. (2000). “Involvement of brain derived neurotrophic factor in spatial memory formation and maintenance in a radial arm maze test in rats”. J. Neurosci, 20, 7116–7121. 
22. Nakagawa, T. Tsuchida, A. Itakura, Y. Nonomura, T. Ono, M. Hirota, F. Inoue, T. Nakayama C, Taiji M, Noguchi H. (2000). “Brain-derived neurotrophic factor regulates glucose metabolism by modulating energy balance in diabetic mice”. Diabetes, 49, 436–444.
23. Nelson TL, Hokanson JE, Hickey MS. (2008). “Omega-3 fatty acids and lipoprotein associated phospholipase A2 in healthy older adult males and females”. Eur J Nutr, 50,185–193.
24. Palmquist D. L. (2009). “Omega-3 Fatty Acids in Metabolism, Health, and Nutrition and for Modified Animal Product Foods”, The Professional Animal Scientist, 25, 207–249.
25- Schiffer T, Schulte S, Hollmann W, Bloch W, Strüder HK. (2009). “Effects of strength and endurance training on brain-derived neurotrophic factor and insulin-like growth factor 1 in humans”. Horm Metab Res, 41(3),250-254.
26. Szatmari, E. Kalita, K. B. Kharebava, G, & Hetman, M. (2007). “Role of kinase suppressor of Ras-1 in neuronal survival signaling by extracellular signal regulated kinase ½”. Journal of Neuroscience, 27(42). 11389–11400.
27- Witte A, Kerti L, Hermannstädter H, Fiebach1 J, Schreiber S. (2013). “Long-Chain Omega-3 Fatty Acids Improve Brain Function and Structure in Older Adults”. Cerebral Cortex, 163,243-248.
28. Wu A, Ying Z, Gomez-Pinilla F. (2004). “Dietary omega-3 fatty acids normalize BDNF levels, reduce oxidative damage, and counteract learning disability after traumatic brain injury in rats”. J Neurotrauma; 21(10),1457-1467.
29. Xu, B.; Zang, K.; Ruff, NL.; Zhang, YA.; McConnell, SK.; Stryker, MP. et al. (2000). “Cortical degeneration in the absence of neurotrophin signaling: dendritic retraction and neuronal loss after removal of the receptor TrkB”. Neuron, 26, 233-245.
30. Yarrow JF, White LJ, McCoy SC, Borst SE. (2010). “Training augments resistance exercise induced elevation of circulating brain derived neurotrophic factor (BDNF)”. Neurosci Lett. 479(2),161-165.