Document Type : Research Paper
Assistant Professor, Faculty of Physical Education and Sport Sciences, Shahid Beheshti University, Tehran, Iran
Associate Professor of Exercise Physiology, Department of Physical Education and Sports Sciences, Tarbiat Modares University, Tehran, Iran
Associate Professor of Occupational Health Engineering, Department of Environmental & Occupational Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
PhD in Exercise Physiology, Department of Physical Education and Sport Sciences, Tarbiat Modares University, Tehran, Iran
The present study aimed at evaluating the effect of aerobic exercise in carbon black particulates air pollution on TLR4 and TNF-α gene expression in male rats. 24 male Wistar rats (mean body mass 279.29+26.97 gr) were randomly divided into 4 groups: A) control, B) aerobic exercise, C) pollution, D) exercise+pollution. A particulates injector and Falounak chamber were used to expose the rats to carbon black particulates with a diameter˂10 micron. Aerobic exercise protocol was performed in each group with 50% maximum speed for 4 weeks. The subjects were killed 24 hours after the last session and exposure to carbon black particulates. The gene expression of TLR4, and TNF-α were analyzed in the lung tissue by Real time – Pcr technique. In order to determine the significant differences among the groups, multiple ANOVA, LSD post hoc and non-parametric Friedman tests were used. Aerobic exercise reduced the gene expression of TLR4, and TNF-α in rats exposed to carbon black particulates. Also, the exercise significantly reduced body mass in those groups exposed to carbon black particulates. 4 weeks of aerobic exercise in carbon black particulates air pollution balanced inflammatory markers of TLR4, TNF-α and body mass. It seems that these changes are partly associated with a reduction in the risk of pulmonary diseases.