Effect of eight weeks high intensity interval training on gene expression of PGC-1α, in male healthy rats fast-slow twitch muscles

Document Type : Research Paper


1 Dept. of Sport Physiology, School of Physical Education And Sport Science, Shiraz University, Shiraz, Iran.

2 Department of Exercise Physiology, Tehran University, Tehran, Iran


The aim of the present study was to investigate the effect of HIIT run on PGC-1α gene expression in fast & slow twitch muscles of healthy male  rats. For this purpose, 12 male Wistar rats divided into two groups: contorol (n=6) and exercise (n=6) , respectively. Intense interval training was consists of five days and a total of eight weeks,  that was formed the three frequencies ( 90 to 100% VO2max intensity for four minutes and two minutes at an intensity of 50 to 60% VO2max). 24 hours after the last training session, extensor digitorum longus muscle (EDL) and soleus was extracted and relevant variables and expression levels were measured by RT-PCR. Results showed a HIIT run a significant increase PGC-1α gene expression in both muscle groups were trained (p=0/004 and p=0/001). According to the findings, implement HIIT will be increases PGC-1α and oxidative capacity in SOL and EDL muscles.



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