Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


1 Faculty of physical education and sport science ,University of Tehran

2 Faculty of physical education and sport science university of kerman(M.Sc.)


Introduction: Strength training has an important role in the exercise programs of normal individuals and athletes and it also has numerous benefits on the health of human body and spirit. One of the types of these exercise is eccentric resistance exercise which is associated with negative physiological changes. Thus: this study examined the effects of a single bout of eccentric resistance exercise on the urinary markers of protein catabolism and muscle damage such as urea, creatinine and3-methylhistidine (3Mh).
Material and methods: For this purpose, 24 healthy non-athletes male student volunteers (age; 20±1.54, body mass index 22.6±3.8 and body fat percent 13.41±4.21) selected and were divided randomly into two groups experimental (n=12) and control (n=12) group. After determining the maximum power of the non-dominant hand, Experimental group performed a single bout of eccentric resistance exercise including 50 eccentric contractions as five sets of 10 eccentric contractions with a one minute rest interval between sets, at ٪85 of 1-RM with non- dominant hand. Urinary of samples to assess urea, creatinine and 3-methylhistidine were taken before exercise until 14 hour after intervention. Data were analyzed using independent sample t-test at p Results: Results showed significant increase in urinary urea, creatinine and 3-methylhistidine after an eccentric resistance exercise in experimental group compared to control group.
Discussion: It is concluded that a single bout of eccentric resistance exercise results in an activation of the muscular protein catabolism, and muscle damage due to increase in degradation of myofibrillar contractile protein.


  1. اتکو، ویرو. (1383): پایش بیوشیمیایی تمرین‌های ورزشی، ترجمۀ گائینی، عباسعلی و همکاران، تهران: سمت.
  2. بومپا، تئودوراُ. (1382): زمان‌بندی و طراحی تمرین قدرتی در ورزش، ترجمۀ حمید رجبی و همکاران، تهران: فر دانش‌پژوهان.
  3.  موان رن، گلیسون مایکل. (1380): بیوشیمی ورزش و تمرین‌های ورزشی، ترجمۀ عسگری، علیرضا و مهران حسینعلی، تهران: نورپردازان.



  1. Behm, D.G., K.M. Baker, R. Kelland, and J. Lomond. (2001) The effect of muscle damage on strength and fatigue deficits. J Strength Cond Res, 15, 255-63.
  2. Calles-Escandon, J., J.J. Cunningham, P. Snyder, R. Jacob, G. Huszar, J. Loke, and P. Felig. (1984) Influence of exercise on urea, creatinine, and 3-methylhistidine excretion in normal human subjects. Am J Physiol, 246, E334-8.
  3. Chen, T.C., K.Y. Lin, H.L. Chen, M.J. Lin, and K. Nosaka. (2011) Comparison in eccentric exercise-induced muscle damage among four limb muscles. Eur J Appl Physiol, 111, 211-23.
  4. Clarkson, P.M. and S.P. Sayers. (1999) Etiology of exercise-induced muscle damage. Can J Appl Physiol, 24, 234-48.
  5. Ebbeling, C.B. and P.M. Clarkson. (1989) Exercise-induced muscle damage and adaptation. Sports Med, 7, 207-34.
  6. Evans, W.J. and J.G. Cannon. (1991) The metabolic effects of exercise-induced muscle damage. Exerc Sport Sci Rev, 19, 99-125.
  7. Falvo, M.J. and R.J. Bloomer. (2006) Review of exercise-induced muscle injury: relevance for athletic populations. Res Sports Med, 14, 65-82.
  8. Friden, J. and R.L. Lieber. (1992) Structural and mechanical basis of exercise-induced muscle injury. Med Sci Sports Exerc, 24, 521-30.
  9. Komulainen, J., J. Kytola, and V. Vihko. (1994) Running-induced muscle injury and myocellular enzyme release in rats. J Appl Physiol (1985), 77, 2299-304.
  10. Paul, G.L., J.P. DeLany, J.T. Snook, J.G. Seifert, and T.E. Kirby. (1989) Serum and urinary markers of skeletal muscle tissue damage after weight lifting exercise. Eur J Appl Physiol Occup Physiol, 58, 786-90.
  11. Pivarnik, J.M., J.F. Hickson, Jr., and I. Wolinsky. (1989) Urinary 3-methylhistidine excretion increases with repeated weight training exercise. Med Sci Sports Exerc, 21, 283-7.
  12. Trappe, T., R. Williams, J. Carrithers, U. Raue, B. Esmarck, M. Kjaer, and R. Hickner. (2004) Influence of age and resistance exercise on human skeletal muscle proteolysis: a microdialysis approach. J Physiol, 554, 803-13.
  13. Tuma, P., E. Samcova, and P. Balinova. (2005) Determination of 3-methylhistidine and 1-methylhistidine in untreated urine samples by capillary electrophoresis. J Chromatogr B Analyt Technol Biomed Life Sci, 821, 53-9.
  14. Vesali, R.F., M. Klaude, L. Thunblad, O.E. Rooyackers, and J. Wernerman. (2004) Contractile protein breakdown in human leg skeletal muscle as estimated by [2H3]-3-methylhistidine: a new method. Metabolism, 53, 1076-80.
  15. Viru, A. and N. Seli. (1992) 3‐Methylhistidine excretion in training for improved power and strength. Sports Medicine, Training and Rehabilitation, 3, 183-193.
  16. Wyss, M. and R. Kaddurah-Daouk. (2000) Creatine and creatinine metabolism. Physiol Rev, 80, 1107-213.