Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


1 Assistant Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

2 M.Sc. of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

3 PhD Student of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

4 PhD Student of Exercise Physiology, Department of Exercise Physiology, Alborz Campus of University of Tehran, Karaj, Iran

5 PhD Student of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Guilan, Iran


Retinol Binding Protein4 (RBP4) Such as adipocytokines that could be a marker for type 2 diabetes And seems to be leading to increased insulin resistance. The purpose of this study was to evaluate the effect of aerobic training on serum RBP4 and insulin resistance in adult male rats. In the present experimental study 16 Wistar rats were randomly divided to one of the exercise group (n=10) or control group (n=10). The training group performed 8 weeks of aerobic training three times in week. Training protocol began intensity 30% vo2max and 10 minutes and In the last two weeks increased Intensity of training to 75% vo2max and time of training to 45-50 minutes. The data were analyzed via independent t-test and Pearson,s correlation coefficient at P≤0/05. analysis of the data showed that Serum RBP4 levels were significantly decreased after aerobic training (P≤0/05). Insulin, glucose, and insulin resistance showed a significant decrease after aerobic training (P≤0/05). However, Eight weeks of aerobic training were not a significant effect on cholesterol, triglyceride and HDL and only after eight weeks, there were significant reductions in LDL (P≤ 0/05). The relationship between RBP4 with lipid profile and insulin resistance Showed no significant correlation After eight weeks of aerobic training. From these results it can be concluded that aerobic training causes was significantly decreased in serum RBP4 in adults male rats and seems to be improve in insulin resistance Effective factor is Reduction in serum RBP4 levels in adult male rats.


1. سوری، رحمن. حسنی، رنجبر شیرین. وهابی، کبری. شب‌خیز، فاطمه. (1392). تأثیر تمرین تناوبی هوازی بر RBP4 سرم و شاخص مقاومت به انسولین در بیماران دیابتی نوع 2. مجلۀ دیابت و متابولیسم ایران، شمارۀ 4، ص: 397-388.
2. Ahmadi, N., Moghadasi, M., &Nuri, R. (2013). Changes of Serum Retinol Binding Protein 4 Levels Following 8 Weeks Moderate Aerobic Exercise. Asian journal of sports medicine, 4(3),PP:208-12.
3. Blair, S. N., & Church, T. S. (2004). The fitness, obesity, and health equation: is physical activity the common denominator?. Jama, 292(10), PP: 1232-1234.
4. Bobbert, T., Raila, J., Schwarz, F., Mai, K., Henze, A., Pfeiffer, A. Fand et al (2010). Relation between retinol, retinol-binding protein 4, transthyretin and carotid intima media thickness. Atherosclerosis, 213(2),PP: 549-551.
5. Broch, M., Gómez, J. M., Auguet, M. T., Vilarrasa, N., Pastor, R., Elio, I and et al. (2010). Association of retinol-binding protein-4 (RBP4) with lipid parameters in obese women. Obesity surgery, 20(9), 1258-1264, PP:1258-64.
6. Chiefari, E., Paonessa, F., Iiritano, S., Le Pera, I., Palmieri, D., Brunetti, G and et al. (2009). The cAMP-HMGA1-RBP4 system: a novel biochemical pathway for modulating glucose homeostasis. BMC biology, 7(1), 24.
7. Cho, Y. M., Youn, B. S., Lee, H., Lee, N., Min, S. S., Kwak, S. Hand et al (2006). Plasma retinol-binding protein-4 concentrations are elevated in human subjects with impaired glucose tolerance and type 2 diabetes. Diabetes care, 29(11), PP:2457-61.
8. Choi, K. M., Kim, T. N., Yoo, H. J., Lee, K. W., Cho, G. J., Hwang, T. Gand et al. (2009). Effect of exercise training on A‐FABP, lipocalin‐2 and RBP4 levels in obese women. Clinical endocrinology, 70(4), PP:569-74.
9. Christou, G. A., Tselepis, A. D., &Kiortsis, D. N. (2012). The metabolic role of retinol binding protein 4: an update. Hormone and metabolic research= Hormon-und Stoffwechselforschung= Hormones etmetabolisme, 44(1), PP:6-14.
10. Cummings, D. M., Henes, S., Kolasa, K. M., Olsson, J., & Collier, D. (2008). Insulin resistance status: Predicting weight response in overweight children.Archives of pediatrics & adolescent medicine, 162(8), PP:764-8.
11. DeBoer, M. D. (2013). Obesity, systemic inflammation, and increased risk for cardiovascular disease and diabetes among adolescents: a need for screening tools to target interventions. Nutrition, 29(2), PP: 379-386.
12. Goodman, E., Graham, T. E., Dolan, L. M., Daniels, S. R., Goodman, E. R., & Kahn, B. B. (2009). The relationship of retinol binding protein 4 to changes in insulin resistance and cardiometabolic risk in overweight black adolescents.The Journal of pediatrics, 154(1), PP:67-73 e1.
13. Graham, T. E., Yang, Q., Blüher, M., Hammarstedt, A., Ciaraldi, T. P., Henry, R. Rand et al (2006).Retinol-binding protein 4 and insulin resistance in lean, obese, and diabetic subjects. New England Journal of Medicine,354(24), PP:2552-63.
14. Henrissen, E. J. (2002). Exercise effects of muscle insulin signaling and action. Invited review: effects of acute exercise and exercise training on insulin resistance. J ApplPhysiol, 93, PP:788-96.
15. Hirshman, M. F., Goodyear, L. J., Horton, E. D., Wardzala, L. J., & Horton, E. S. (1993). Exercise training increases GLUT-4 protein in rat adipose cells.American Journal of Physiology-Endocrinology And Metabolism, 264(6), PP:882–9.
16. Ingelsson, E., Ärnlöv, J., Lind, L., &Sundström, J. (2006). Metabolic syndrome and risk for heart failure in middle-aged men. Heart, 92(10), PP:1409-1413.
17. Kershaw, E. E., & Flier, J. S. (2004). Adipose tissue as an endocrine organ.The Journal of Clinical Endocrinology & Metabolism, 89(6), PP:2548-56.
18 .Kondo, T., Kobayashi, I., & Murakami, M. (2006). Effect of exercise on circulating adipokine levels in obese young women. Endocrine journal, 53(2), PP:189-95.
19. Lakka, H. M., Laaksonen, D. E., Lakka, T. A., Niskanen, L. K., Kumpusalo, E., Tuomilehto, J., &Salonen, J. T. (2002). The metabolic syndrome and total and cardiovascular disease mortality in middle-aged men. Jama, 288(21), PP:2709-16.
20. Lempiäinen, P., Mykkänen, L., Pyörälä, K., Laakso, M., &Kuusisto, J. (1999). Insulin resistance syndrome predicts coronary heart disease events in elderly nondiabetic men. Circulation, 100(2), PP:123-8.
21. Lim, S., Choi, S. H., Jeong, I. K., Kim, J. H., Moon, M. K., Park, K. Sand et al. (2008). Insulin-sensitizing effects of exercise on adiponectin and retinol-binding protein-4 concentrations in young and middle-aged women. The Journal of Clinical Endocrinology & Metabolism, 93(6),PP:2263-8.
22. Mansouri, M., Keshtkar, A., Hasani-Ranjbar, S., Soleymani Far, E., Tabatabaei-Malazy, O., Omidfar, K., &Larijani, B. (2011).The impact of one session resistance exercise on plasma adiponectin and RBP4 concentration in trained and untrained healthy young men. Endocrine journal, 58(10), PP:861-8.
23. Numao, S., Sasai, H., Nomata, Y., Matsuo, T., Eto, M., Tsujimoto, T., & Tanaka, K. (2012). Effects of exercise training on circulating retinol-binding protein 4 and cardiovascular disease risk factors in obese men. Obesity facts,5(6), PP:845-55.
24. Ribel-Madsen, R., Friedrichsen, M., Vaag, A., &Poulsen, P. (2009). Retinol-binding protein 4 in twins regulatory mechanisms and impact of circulating and tissue expression levels on insulin secretion and action. Diabetes, 58(1), PP:54-60.
25. Ross, R., Freeman, J. A., & Janssen, I. (2000). Exercise alone is an effective strategy for reducing obesity and related comorbidities. Exercise and sport sciences reviews, 28(4), PP:65-70.
26. Sigal, R. J., Kenny, G. P., Wasserman, D. H., Castaneda-Sceppa, C., & White, R. D. (2006). Physical activity/exercise and Type 2 diabetes A consensus statement from the American Diabetes Association. Diabetes care, 29(6), PP:1430-8.
27. Stallknecht, B., Andersen, P. H., Vinten, J., Bendtsen, L. L., Sibbersen, J., Pedersen, O., &Galbo, H. (1993). Effect of physical training on glucose transporter protein and mRNA levels in rat adipocytes. American Journal of Physiology-Endocrinology And Metabolism, 265(1), PP:E128– 34.
28. Takashima, N., Tomoike, H., & Iwai, N. (2006).Retinol-binding protein 4 and insulin resistance. N Engl J Med, 355(13), PP:1393–4.
29. Tatemoto, K., Takayama, K., Zou, M. X., Kumaki, I., Zhang, W., Kumano, K., &Fujimiya, M. (2001). The novel peptide apelin lowers blood pressure via a nitric oxide-dependent mechanism. Regulatory peptides, 99(2), PP:87-92.
30. Von Eynatten, M., Lepper, P. M., Liu, D., Lang, K., Baumann, M., Nawroth, P. Pand et al. (2007). Retinol-binding protein 4 is associated with components of the metabolic syndrome, but not with insulin resistance, in men with type 2 diabetes or coronary artery disease. Diabetologia, 50(9), PP:1930-7.
31. Wu, Y., Li, H., Loos, R. J., Qi, Q., Hu, F. B., Liu, Y., & Lin, X. (2009). RBP4 variants are significantly associated with plasma RBP4 levels and hypertriglyceridemia risk in Chinese Hans. Journal of lipid research, 50(7),PP:1479–86.
32. Yang, Q., Graham, T. E., Mody, N., Preitner, F., Peroni, O. D., Zabolotny, J. M and et al.(2005). Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature, 436(7049), PP:356-62.
33. Yao-Borengasser, A., Varma, V., Bodles, A. M., Rasouli, N., Phanavanh, B., Lee, M. J and et al. (2007). Retinol binding protein 4 expression in humans: relationship to insulin resistance, inflammation, and response to pioglitazone. The Journal of Clinical Endocrinology & Metabolism, 92(7), PP:2590-7.