Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license
Authors
- Bahloul Ghorbanian 1
- Mohamad Reza Kordi 2
- Ali Asghar Ravasi 3
- Mehdi Hedayati 4
- Agha Ali Ghasemnian 5
1 Assistant Professor, Physical Education and Sports Sciences Department, Faculty of Educational Sciences and Psychology, Azerbaijan Shahid Madani University, Tabriz, Iran
2 Associate Professor, Exercise Physiology Department, Physical Education and Sports Sciences Faculty, University of Tehran, Tehran, Iran
3 Professor, Exercise Physiology Department, Physical Education and Sports Sciences Faculty, University of Tehran, Tehran, Iran
4 Associate Professor of Biochemistry, Cellular-Molecular Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Assistant Professor, Department of Exercise Physiology, Faculty of Humanities, University of Zanjan, Zanjan, Iran
Abstract
Abstract
Back grand: Early obesity and its transfer to the adult, increases likelihood incidence of coronary artery disease (CAD). ATP-binding cassette transporter) ABCA1) as a member of the ABC transporters family plays a crucial role in reverse cholesterol transport and CAD prevention.
Objective: The purpose of this study was to investigate ABCA1 expression in lymphocytes, plasma apolipoprotein A-I and lipid profiles in response to eight weeks interval aerobic endurance exercise as rope training.
Materials and Methods: Thirty students (17.3±1.1yr, 85.73± 11.68 kg and 28.41± 2.36 kg / m²) volunteered and randomly were assigned into training (n = 15) and control (n =15) groups. Exercise protocol was endurance rope training (8 wk, 4 d/wk and 40 min/d). cell hemolysis and sensitive Elisa method was used for Lymphocyte ABAC1 protein expression.T-student test was employed and data were analyzed by SPSS software (version 16).
Results: The independent-samples T-Test results were showed that after 8 weeks interval rope endurance training, the levels of lymphocyte ABCA1 expression(p=0/000) and VO2max(p=0/000) were significantly increased and plasma levels of TG(p=0.017), TC(p=0.001), LDL-c/HDL-c(0.026),TC/HDL-c(p=0.002) and measures of BF%(p=0/015)and BMI(p=0.042) as anthropometric indicators were decreased. Changes of other variables such increasing in Apo A-I , HDL-c, LDL-c and decreasing in body weight, were not significant. Significant level (p>0.05) was considered.
Conclusion: The findings of this study proved that 8-week interval aerobic endurance exercise as rope training can be have positive effects on lymphocyte ABCA1 protein expression(as goalkeeper of reverse cholesterol process) and lipid profiles and ultimately in prevention of arteriosclerosis among overweight and obesity adolescents.
Keywords
- Abe-Dohmae S, Suzuki S, Wada Y, Aburatani H, Vance DE, Yokoyama S. Characterization of apolipoprotein-mediated HDL generation induced by cAMP in a murine macrophage cell line. Biochemistry. 2000 Sep; 39(36):11092-9
- Amiri P, Ghofranipour F. Ahmadi F & et al. Barriers to lifestyle modification in obese or overweight adolescents. The Iranian J Endocrinol Metab ( Shahid Beheshti University of Medical Sciences and Health Services).2009,12(5): 529 – 21.
- Atlantis E, Barnes EH and Fiatarone Singh MA. Efficacy of exercise for treating overweight in children and adolescents: a systematic review. Int J Obes (Lond). 2006 Jul; 30 (7):1027-40.
- Attie AD, Kastelein JP, Hayden MRPivotal role of ABCA1 in reverse cholesterol transport influencing HDL levels and susceptibility to atherosclerosis. J Lipid Res. 2001 Nov;42(11):1717-26
- Barger PM, Kelly DP. PPAR signaling in the control of cardiac energy metabolism. Trends Cardiovasc Med. 2000; 10(6):238-45.
- Barzin M. Myrmyra p. Ramadznkhani A & et al. Obesity prevalence in young men (18 to 25 years) at the time of entry into military service (September 2007). The Iranian J Endocrinol Metab ( Shahid Beheshti University of Medical Sciences and Health Services). 2009,10 (6) : 605 – 613.
- Butcher. L, A. Thomas and K. Backx. Low-Intensity Exercise Exerts Beneficial Effects on Plasma Lipids via PPAR [gamma]. Medicine and Science in Sports and Exercise, 2008; 40(7):1263-74
- Büttner P, Mosig S, Lechtermann A, Funke H, Mooren FC. Exercise affects the gene expression profiles of human white blood cells. J Appl Physiol 2007; 102 (1):26–36.
- Chao-Chien C, Shih-Yen L. The impact of rope jumping exercise on physical fitness of visually impaired students. Department of Physical Education, Asia University, Taiwan, Research in Developmental Disabilities. Research in Developmental Disabilities. 2011; (32):25–29
- Chinetti-Gbaguidi G, Rigamonti E, Helin L, Mutka AL, Lepore M,Fruchart JC, et al. Peroxisome proliferator-activated receptor alpha controls cellular cholesterol trafficking in macrophages.J Lipid Res. 2005;46(12):2717-25.
- Chinn S, Rona RJ. Prevalence and trends in overweight and obesity in three cross sectional studies of British Children, 1974–94. BMJ 2001; 322: 24–26.
- Crouse SF, O'Brien BC, Grandjean PW, Lowe RC,ohack JJ, Green JS. Effects of training and a single session of exercise on lipids and apolipoproteins in hypercholesterolemic men. J Appl Physiol 1997; 83.2019-28.
- Eckardstein AV, Jerzy-Roch N and Gerd A. High Density Lipoproteins and Arteriosclerosis. Role of Cholesterol Efflux and Reverse Cholesterol Transport. Arterioscle Thromb Vasc Biol. (2001), 21: 13-27.
- Eun Sung Kim, Jee-Aee Im, Kyoung Chul Kim, Ji Hye Park et al .Improved Insulin Sensitivity and Adiponectin Level after Exercise Training in Obese Korean Youth. Obesity (Silver Spring, Md) 2007, 15(12):3023-30.
- Fatone C, Guescini M, Balducci S, Battistoni S, Settequattrini A, Pippi R, et al. Two weekly sessions of combined aerobic and resistance exercise are sufficient to provide beneficial effects in subjects with Type 2 diabetes mellitus and metabolic syndrome.J Endocrinol Invest. 2010; 33(7):489-95
- Francis GA, Annicotte JS, Auwerx J. PPAR-alpha effects on the heart and other vascular tissues. Am J Physiol Heart Circ Physiol. 2003; 285(1):H1-9.
- Freedman D. S, Dietz W. H, Srinivasan S. R and Berenson G. S. The relation of overweight to cardiovascular risk factors among children and adolescents: The Bogalusa Heart Study. Pediatrics1999, 103, 1175–11.
- Gene Adams. Exercise Physiology Laboratory Manual (4thed). McGraw-Hill Publishers, New York, NY. 2002
- Ghanbari-Niaki A, Saghebjoo M, Hedayati M. A single session of circuit-resistance exercise effects on human peripheral blood lymphocyte ABCA1 expression and plasma HDL-C level. Regul Pept. (2011); 166 (1-3):42-7.
- Gordon DJ, Probstfield JL, Garrison RJ & et al. High-density lipoprotein cholesterol and cardiovascular disease: four prospective American studies. Circulation 1989; 79:8-15.
- Hoang A, Tefft C and Duffy S. ABCA1 expression in humans is associated with physical activity and alcohol consumption.atherosclerosis,2008:197(1) :197-203
- Jackson AS, Pollock ML. Generalized equations for predicting body density of men. Br J Nutr. 1978; 40: 497–504
- Jennifer Ann McKenzie. The influence of visfatin and visfatin gene polymorphisms on glucose and obesity-related variables and their responses to aerobic exercise training. Department of Kinesiology. 2008; 20742-7011 (301)314-328.
- Jerry Shearman, Dominic Micklewright, Jane Hardcastle, Michael Hamlin. The Effect of Physical Activity on Serum Lipids, Lipoprotein, and. Apolipoproteins. Arch Exerc Health Dis. 2010; 1 (2):43-49.
- Khabazian BM, Ghanbari-Niaki A, Rahbarizadeh F & et al. Effect of 6 weeks of endurance training on ABCA1 expression in rats liver. Journal of Sports research,2008,vol:(18)101-114
- Khabazian BM, Ghanbari-Niaki A, Safarzadeh-Golpordesari A, Ebrahimi M, Rahbarizadeh F, Abednazari H. Endurance training enhances ABCA1 expression in rat small intestine. Eur J Appl Physiol. 2009 Oct; 107(3):351-8.
- Khalil M, William D, Wagner and Goldberg IJ. Molecular Interactions Leading to Lipoprotein Retention and the Initiation of Atherosclerosis. Arterioscler Thromb Vasc Biol. 2004, 24: 2211-18.
- Kishali NF, Imamoglu O, Kaldirimci M, Akyol P, Yildirim K. Comparison of lipid and lipoprotein values in men and women differing in training status. Int J Neurosci. 2005; 115:1247-57.
- Lee IM, Hsieh CC, Paffenbarger RS. Exercise intensity and longevity in men. The Harvard Alumni Study. JAMA. (1995); 273:1179–1184
- Leon AS, Rice T, Mandel S, Després JP, Bergeron J,Gagnon J, et al. Blood lipid response to 20 weeks of supervised exercise in a large biracial population: the Hertage Family Study. Metabolism 2000; 49:513-20.
- Matsuura F, Oku H, Koseki M, Sandoval JC, Yuasa-Kawase M, Tsubakio-Yamamoto K, et al. Adiponectin accelerates reverse cholesterol transport by increasing high density lipoprotein assembly in the liver. Biochem Biophys Res Commun. 2007; 358 (4):1091-5
- Mirmohammadi S, Hafezi R, Mehrparvar A, Rezaeian .B, Akbari H. Prevalence of Overweight and Obesity among Iranian School Children in Different Ethnicities, Iranian Journal of Pediatrics. 2011; 21(4).515-520
- Mitsuzono R, Ube M. Effects of endurance training on blood lipid profiles in adolescent female distance runners Kurume Med J. 2006; 53(1-2):29-35.
- Ogata M, Tsujita M, Hossain MA, Akita N, Gonzalez FJ, Staels B & et al. On the mechanism for PPAR agonists to enhance ABCA1 gene expression. Atherosclerosis. 2009; 205(2):413-19.
- Oku H, Matsuura F, Koseki M, Sandoval JC, Yuasa-Kawase M,Tsubakio-Yamamoto K, et al. Adiponectin deficiency suppressesABCA1 expression and ApoA-I synthesis in the liver. FEBS Lett. 2007; 581(26):5029-33.
- Olchawa Tall A.R, Xian-cheng Jiang, Yi Luo, Silver D George Lyman Duff Memorial Lecture. Lipid Transfer Proteins, HDL Metabolism, and Atherogenesis. Arterioscler Thromb Vasc Bio. (2000) 20: 1185-1188.
- Patel DC, Albrecht C, Pavitt D, Paul V, Pourreyron C, et al. Type 2 Diabetes Is Associated with Reduced ATP-Binding Cassette Transporter A1 Gene Expression, Protein and Function. PLoS ONE (2011): 6(7): e22142. doi:10.1371/journal.pone.0022142
- Rashidlamir A, Ghanbari-Niaki A, Saghebjoo M. The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-I. World Journal of Sport Sciences, 2011; 4 (2): 144-150.
- Sahoo D, Timothy C, Trischuk, TC, Victor A B, Samuel Ho & et al .ABCA1-dependent lipid to efflux to apolipoprotein A-I mediates HDL particle formation and decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research. (2004) 45: 1122-31
- Sajad A, Amir H Mohammad Reza H. Effects of resistance versus endurance training on serum adiponectin and insulin resistance index , Shahid Beheshti University, Tehran, Eur J Endocrinol, 2007 ; 157: 625-631
- Siahkohian M, L. Bolboli and A. Naghizadeh Baghi. The Effects of Exercise Intensity on the Low-Density Lipoprotein Profile: Quantitative vs. Qualitative Changes. Journal of Biological Sciences 2008; 8:335-341
- Spangenburg EE, Brown DA, Johnson MS, Moore RL. Alterations in peroxisome proliferator-activated receptor mRNA expression in skeletal muscle after acute and repeated bouts of exercise.Mol Cell Biochem. 2009; 332(1-2):225-31
- Steel CM, Evans J, Smith MA. Physiological variation in circulating B cell: T cell ratio in man. Nature 1974; 247: 387–9.
- Van Eck M, Bissada N, Zimetti F, Collins HL, Hildebrand RB, Hayden A, Brunham LR, Kang MH, Fruchart JC, Van Berkel TJ, Parks JS, Staels B,Rothblat GH, Fiévet C, Hayden MR. Both hepatic and extrahepatic ABCA1 have discrete and essential functions in the maintenance of plasma high-density lipoprotein cholesterol levels in vivo. Circulation. 2006 Sep 19; 114 (12):1301-9.
- William, Haskell et al. Physical Activity and Public Health. Updated Recommendation for Adults From the American College of Sports Medicine and the American Heart Association. 2007; 10.1161.70.
- William E and at al. Effects of the amount and intensity of exercise on plasma lipoproteins. N Engl J Med, November 2002; 347(19):112-121.
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