The Effects of 8 Weeks of Intermittent Training on Lactate (La) Level and Lactate Dehydrogenase (LDH) Enzyme Activity in Male Wistar Rats

Arabmomeni, Allahyar; Mohebi, Hamid; Rahmani Nia, Farhad; Riasi, Ahmad

doi:10.22059/jsb.2015.55231

Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license

Authors

¹ PhD of Exercise Physiology, Faculty of Human Sciences, Islamic Azad University, Khomeinishahr Branch, Khomeinishahr, Isfahan, Iran

² Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran

³ Assistant Professor of Animal Physiology, Faculty of Agriculture, Industrial University of Isfahan, Isfahan, Iran

https://doi.org/10.22059/jsb.2015.55231

Abstract

The aim of this study was to assess the effects of intermittent training on lactate level and lactate dehydrogenase enzyme activity in Wistar rats. 20 male Wistar rats (mean age 3 months and weight 224±14 g) were selected and randomly divided into the training (n=10) and control (n=10) groups. The training protocol consisted of running on a treadmill for 4 minutes and then 2 minutes of active rest in 10 training phases for the experimental group. All rats were anesthetized with a mixture of ketamine and xylazine 48 hours after the last training session after an overnight fasting. To measure lactate and LDH enzyme activity, blood samples were obtained from their cardiac puncture. Data were analyzed by mean and standard deviation (M+SD) and independent t test. The results showed no significant differences in blood lactate level between the two groups, but there was a significant difference in LDH enzyme activity between the two groups (P˂0.05). These results indicate that intermittent training caused the clearance of lactate. Enhance of lactate replenished muscle glycogen and prevented H+ concentration which was produced along with lactate.

Keywords

References

1. 1. Burgomaster KA, Cermak NM, Phillips SM, Benton CR, Bonen A, Gibala MJ. (2007). Divergent response of metabolite transport proteins in human skeletal muscle after sprint interval training and detraining, Am J Physiol Regul Integr Comp Physiol 292: R1970–R1976,
2. 2. Choung BY, Byun SJ, Suh JG, Kim TY. (2004). Extracellular superoxide dismutase tissue distribution and the patterns of superoxide dismutase mRNA expression following ultraviolet irradiation on mouse skin. Exp Dermatol; 13(11):691-9.
3. 3. Clarkson.P. Kearns.A., Rouzier.P. Rubin.R & Thompson. P. (2006). Serum creatinekinase levels and renal function measures in exertional muscle damage. Pediatric critical care medicine.38 (4):623-627.
4. 4. Clarkson, P.M., and H.S. Thompson (2000). “Antioxidants: What Role Do They Play in Physical Activity and Health?” American Journal of Clinical Nutrition 72: 637-646.
5. 5. Carnevali Jr. L.C, Eder R, Lira F.S, Lima. W.P, Gonçalves. D.C, Zanchi. N.E, Nicastro. H, Lavoie J.M, and Seelaender. M.C.L, (2012). Effects of high-intensity intermittent training on carnitine palmitoyl transferase activity in the gastrocnemius muscle of rats, Brazilian Journal of Medical and Biological Research 45: 777-783
6. 6. Cupeiro, R., Benito, P. J., Maffulli, N., Calderon, F. J., & Gonzalez-Lamuno, D. (2010). MCT1 genetic polymorphism influence in high intensity circuit training: A pilot study. Journal of Science and Medicine in Sport / Sports Medicine Australia, 13, 526-530.
7. 7. Daussin FN, Zoll J, Dufour SP. (2008). Effect of interval versus continuous training on cardiorespiratory and mitochondrial function: relationship to aerobic performance improvement in sedentary subjects. Am J Physiol Regul Integr Comp Physiol; 295(1): R264-72.
8. 8. Díaz-Herrera P, Torres A, Morcuende JA, García-Castellano JM, Calbet JA, Sarrat R. (2001). Effect of endurance running on cardiac and skeletal muscle in rats. Histol histopathol.;16(1):29-
9. 9. Donovan CM, Brooks GA. (1983). Endurance training affects lactate clearance, not lactate production. Am Journal Physiology Endocrinal Metabolism. 244(1):83-9.
10. 10. Dubouchaud H, Butterfield GE, Wolfel EE, Bergman BC, Brooks GA. ,(2000). Endurance training, expression, and physiology of LDH, MCT1, and MCT4 in human skeletal muscle. Am J Physiol Endocrinol Metab 278: E571–E579
11. 11. Gladden LB. (2004.). Lactate metabolism: a new paradigm for the third millennium. J Physiol 558: 5–30.
12. 12. Groussard C, Rannou-Bekono F, Machefer G, Chevanne M, Vincent S, Sergent O, et al. (2003). Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise. Eur J Appl Physiol; 89(1):14-20.
13. 13. Hafstad AD, Boardman ND, Lund J, Hagve M, Khalid AM, Wisløff U, , Larsen TS, Aasum E. (.2011). High intensity interval training alters substrate utilization and reduces oxygen consumption in the heart. J Appl Physiol 111: 1235–1241.
14. 14. Hamada, Taku and Takimoto, Masaki, (2013). Regulation of the exercise-induced expression of the monocarboxylate transporters MCT1 and MCT4 in skeletal muscle, 2013, J Phys Fitness Sports Med, 2(1): 85-92.
15. 15. Horita, T., N.C. Komi, C. Nicol, and H. Kyrolainen (1999). Effect of Exhausting Stretch Shorting Cycle Exercise on the Time Course of Mechanical Behavior in Drop Jump”. European Journal of Applied Physiology and Occupation Physiology. 79: 160-167.
16. 16. Juel C, Klarskov C, Nielsen JJ, Krustrup P, Mohr M, Bangsbo J.(2004). Effect of high-intensity intermittent training on lactate and H_ release from human skeletal muscle. Am J Physiol Endocrinol Metab 286:E245–E251.
17. 17. Kay, B. (2008). "Bicarbonate as an ergogenic aids? A physical, chemical, mechanistic view point". Brezilian journal of biomotricity, 16:205-219.
18. 18. Kelley KM, Hamann JJ, Navarre C, Gladden LB. (2002). Lactate metabolismin resting and contracting canine skeletal musclewith elevated lactate concentration. J Appl Physiol; 93:865–72.
19. 19. Mashiko.T. T.Umeda.,S.Nakaji & K.Sugawara, (2004). Effects of exercise on the physical condition of college rugby players during summer training camp. Br J Sports Med.38:186-190.
20. 20. Matsuse H , Shiba N, Umezu Y, Nago T, Maeda T, Tagawa Y, Matsuo S, Nagata K, Basford JR(2006). Effects of a hybrid exercise on the activities of myogenic enzymes in plasma. Kurume Med J.; 53(3-4):47-51.
21. 21. Mohr M, Krustrup P, Nielsen JJ, Nybo L, Rasmussen MK, Juel C, Bangsbo J. ,(2007). Effect of two different intense training regimens on skeletal muscle ion transport proteins and fatigue development. Am J Physiol Regul Integr Comp Physiol 292: R1594–R1602.
22. 22. Pesce, A., McKay, R.H., Stolzenbach, F., Cahn, R.D., Kaplan, N.O. (1994). The comparative enzymology of lactic dehydrogenases. I. Properties of the crystalline beef and chicken enzymes. J. Biol. Chem. 239, 1753–1761
23. 23. Rog´erio Santos de Oliveira Cruz, et al. (2012). Intracellular Shuttle: The Lactate Aerobic Metabolism, The Scientific World Journal Volume 2012, Article ID 420984, 8 pages, doi:10.1100/2012/420984.
24. 24. Sari, Dewi N.; Endardjo, Sutjahjo; Santoso Dewi I.S. (2013). Blood lactate level in Wistar rats after four and twelve weeks intermittent aerobic training, Med J Indones.; 22:141-5. doi: 10.13181/mji.v22i3.582
25. 25. Schantz, P.G. (1998). “Plasticity of Human Skeletal Muscle with Special Reference to Effects of Physical Training on Enzyme Levels”. Acta Physiologica Scandinavica. Supplementum. 558:1-62.
26. 26. Schmutz S, Dapp C, Wittwer M, Durieux AC, Mueller M, Weinstein F, Vogt M, Hoppeler H, Fluck M. A (2010). Hypoxia complement differentiates the muscle response to endurance exercise. Exp Physiol 95: 723–735.
27. 27. Siu PM, Donley DA, Bryner RW, Alway SE. (2003). Citrate synthase expression and enzyme activity after endurance training in cardiac and skeletal muscles. J Appl physiol; 94(2):555-60.
28. 28. Tiidus, P.M. J. Pushkarenko, and M.E. Houston. (1996). “Lack of Antioxidant Adaptation to Short Term Aerobic Training in Human Muscle”. American Journal of Physiology. 271(4 Pt 2): R832-836.
29. 29. Washington, Tyrone A.; Dameon A. Lemuel; Brown, Gina; Davis, Smith; Baum, Jamie & Bottje Walter. (2013). Monocarboxylate transporter expression at the onset of skeletal muscle regeneration, Physiological Reports, 1 (4), e00075, doi: 10.1002/phy2.75.

Journal of Sport Biosciences

The Effects of 8 Weeks of Intermittent Training on Lactate (La) Level and Lactate Dehydrogenase (LDH) Enzyme Activity in Male Wistar Rats

References

References

Volume 7, Issue 2 - Serial Number 2
August 2015
Pages 297-309

The Effects of 8 Weeks of Intermittent Training on Lactate (La) Level and Lactate Dehydrogenase (LDH) Enzyme Activity in Male Wistar Rats

References

References

Volume 7, Issue 2 - Serial Number 2August 2015Pages 297-309

Volume 7, Issue 2 - Serial Number 2
August 2015
Pages 297-309