Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license


1 PhD of Exercise Physiology, Faculty of Human Sciences, Islamic Azad University, Khomeinishahr Branch, Khomeinishahr, Isfahan, Iran

2 Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran

3 Assistant Professor of Animal Physiology, Faculty of Agriculture, Industrial University of Isfahan, Isfahan, Iran


The aim of this study was to assess the effects of intermittent training on lactate level and lactate dehydrogenase enzyme activity in Wistar rats. 20 male Wistar rats (mean age 3 months and weight 224±14 g) were selected and randomly divided into the training (n=10) and control (n=10) groups. The training protocol consisted of running on a treadmill for 4 minutes and then 2 minutes of active rest in 10 training phases for the experimental group. All rats were anesthetized with a mixture of ketamine and xylazine 48 hours after the last training session after an overnight fasting. To measure lactate and LDH enzyme activity, blood samples were obtained from their cardiac puncture. Data were analyzed by mean and standard deviation (M+SD) and independent t test. The results showed no significant differences in blood lactate level between the two groups, but there was a significant difference in LDH enzyme activity between the two groups (P˂0.05). These results indicate that intermittent training caused the clearance of lactate. Enhance of lactate replenished muscle glycogen and prevented H+ concentration which was produced along with lactate.                   



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