The effect of 6 weeks endurance training on the levels of some cytokines in tumor microenvironment in breast cancer bearing mice

Document Type : Research Paper


1 Associate Professor of Exercise Physiology, Tarbiat Modares University, Tehran, Iran

2 MSc of Exercise Physiology, Tarbiat Modares University, Tehran, Iran

3 PhD of Immunology, Pasteur Institute of Iran, Tehran, Iran

4 PhD of Exercise Physiology, Tarbiat Modares University, Tehran, Iran

5 PhD of Exercise Physiology, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran

6 MSc of Exercise Physiology, Islamic Azad University of Kerman, Science and Research Branch, Kerman, Iran


The aim of this study was assessing the adjuvant therapy of exercise on cytokines balance in mice with breast cancer tumor. Twenty female Balb/C mice were randomly assigned to experimental and control groups. After orientation in the environment, estrogen-receptor dependent breast cancer cells (MC4-L2) were injected to them and experimental group performed endurance training 5 day in week for 6 weeks and with moderate intensity. Mice were daily evaluated about tumor growth. Finally, the mice were sacrificed; tumor tissue was removed and immediately frozen and kept in -70°C. Tumor sample was homogenized and levels of IL-17 and IFN- were measured and quantified using ELISA. There was significant decrease on level of IL-17 in experimental group than control group (p=0.03). Also, level of IFN-γ was increased, but it wasn’t significant (p=0.55). Tumor volume reduced in experimental group than control group (p=0.005). Although, IL-17 involved in angiogenesis, development and metastasis, decreased level of this cytokine along with reduced tumor volume shows exercise lead to reduction of tumor growth by decreasing of this cytokine. Also, increase in level of IFN-γ in experimental group shows exercise training lead to induction anti-tumor cell-mediated immunity by cytokines modification in intra-tumor that show protective effect of exercise training at anti-tumor immunity. We can conclude endurance training has effective role at inhibition of tumor growth in estrogen-receptor dependent cancers.


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