Exercise Training has been shown to decrease chronic low- grade systemic inflammation in high- fat diet induced obesity. However, the mechanisms mediating its beneficial effects are not fully understood. Therefore, the aim of this study was to investigate the effects of a 12- week endurance training on IL-6, IL-10 and nesfatin -1 in obese male rats. Sixteen male wistar rats (2 months old) were fed a high fat diet for 8 weeks, reaching an average weight of 319 ± 30g. The animals were randomly divided into control (n=8) and 12-week endurance training (T, n=8) groups. Training group was given exercise on motor- driven treadmill at 25 to 30 m/min (0% grade), 60 min/day, 5 days/week for 12 weeks, and both groups were given “ad libitum”. The Elisa method was used for laboratory analysis of Nesfatin-1, IL-6 and IL-10. The results revealed that the average weight of rats in T group was significantly lower than that of the control group (P=0.001). A significant decrease was also observed in IL-6 T group (P=0.04). Nesfatin – 1 plasma level increased in T group (P=0.001). Despite the fact that IL-10 was lower in T group, the difference was not significant. The results of present study showed that long- term endurance training led to an increase in the anti- inflammatory nesfatin -1, and nesfatin- 1 probably plays a role in energy balance, that is independent of the changes of IL-10 and IL-6 in plasma level.