The purpose of this study was to study the effects of the 12-week resistance training program on some physical fitness and inflammatory markers factors in overweight men. In a semi-empirical trial, 25 young male (age= 20.9 ± 1.96 and height= 170.34 ± 7.39 cm) were purposefully selected and randomized in two groups of resistance training (n=15) and control (n=10) groups. The resistance training was performed with different intensities for a period of 12 weeks, three sessions a week. The resistance training consists of 6 stationary circular motions. 5 ml of venous blood was collected in fasting state before and 24 hours after the last training session to determine inflammatory markers. Independent and paired t test indicated that weight and BMI of subjects did not change significantly after 12 weeks of activity (P> 0.05). But some physical fitness factors (1-RM leg press, VO2 peak, 1-RM bench press) increased, and some anthropometric factors (WH and percentage of body fat) decreased significantly, after 12 weeks (P <0.05). The Exercise group showed significant reductions in insulin resistance, leptin level and tumor necrosis factor-alpha (TNF-α) (P <0.05). Pearson correlation coefficient showed a significant relationship between TNF-α with BMI, W / H, percentage of body fat and insulin (P <0.05). Based on the results of this study, it was found that 12 weeks of resistance training can improve some physical fitness and anthropometric factors and also inflammatory markers in sedentary overweight young men.