The aim of the present study was to examine the effect of a bout of long prior aerobic exercise with 70% VO2max on inflammatory marker (sVCAM-1) and the lipid profile following high fat meal consumption in non–athlete men. Plasma concentrations of adhesion molecules and lipid profile are among the most important indicators of the risk of cardiovascular diseases. For this purpose, 20 non–athlete young men were randomly selected and divided into two groups of experimental (21.98+1.30 years old, 18.04+2.48 body weight percent) and control (22.06+1.22 years old, 18.15+3.54 body weight percent). The experimental group completed a 90–min. treadmill exercise with a definite intensity. A day later, both groups received high fat meal. Blood samples were collected in 30 min. before and 30 min., 1, 3 and 24 hours following the meal. To determine normality of the groups, one-sample Kolmogorov Smirnov (PCON=0.996) (PEX=0.999) and to determine the homogeneity of variances, Leven test and to examine the results among the groups, independent t test were used. Analysis of variance with repeated measures and LSD post hoc test were used to show differences within a group. The results indicated that one bout of long prior aerobic exercise reduced sVCAM–1 (P=0.029). Also, 30 min. and 24 hours following high fat meal, there was a reduction in sVCAM–1 (P=0.016), (P=0.049). The results also indicated that a bout of long prior aerobic exercise increased the level of HDL-c (P=0.000) but decreased the level of LDL-c (P=0.012), vLDL (P=0.000) and triglycerides (P=0.037). According to the results, high fat meal increases the levels of sVCAM-1 and leads to inflammation and disease. Prior exercise can decrease sVCAM-1 and lipid profile which is accompanied by a probable decrease in cardiovascular diseases.