The aim of this study was to investigate the effect of taurine supplementation for two weeks and three sessions of 90 minutes of intense intermittent exercise on some serum cytokines responses in well-trained soccer players. Therefore, 24 football players under 24 years old from soccer league were selected and divided randomly into taurine (TG=8), placebo (PG=8) and control (CG=8) groups. TG consumed 15 mg of taurine per kilogram of body weight and PG group received the same amount of aspartame daily and both groups preformed a 90-min. soccer specific exercise protocol three times. The CG group received no supplementation performed their ordinary program. Blood samples (5 cc) were gathered in six stages (48 h before the protocol, before and immediately after the first and third protocol and 48 h after the end of the protocol) from the anterior forearm venous in the sitting position. Then they were tested to determine changes in cytokines (IL-6, TNF-α) in the central endocrine laboratory by special kits. One-way ANOVA with repeated measures and Tukey post hoc test with Spss.17 software were used to compare the means in each group. The results showed that serum levels of IL-6 and TNF-α at six stages were measured and there was a significant difference in these variables among all groups as (P=0.029) and (P=0.013) respectively. This difference was significant between the control and placebo group as (P=0.043) and (P=0.008) respectively. It appears that the 90-min. soccer specific protocol three times applies such a pressure on soccer players that may be repeated during the competition season again and again. On the other hand, results showed that taurine supplementation before and during this period had anti-inflammatory effects and the noticeable changes to these cytokines were prevented. Thus, short-term consumption of taurine supplementation during pressure-filled weeks of competition and training is suggested to trained soccer players.