To date, most studies have focused on continuous aerobic training and there is not comprehensive information about the influence of discontinuous aerobic training on cardiovascular risk biomarkers. Taking this into consideration, the purpose of the present study is to investigate the influence of an 8-week discontinuous aerobic training program (3×10 min) on cardiovascular risk biomarkers in inactive obese women. 20 inactive obese women with BMI≥30Kg/m2 volunteered to take part in this study. They were randomly divided into two equal groups of 10 called the discontinuous aerobic group and control group. The training was programmed for 8 weeks, 3 sessions in each week and three 10-min exercises in each session with a 5-min break between each exercise and 60 to 65 percent of maximum heart rate. The blood sampling ELISA method was used to measure the variables required from the discontinuous and control groups before and after the training program at overnight fast. The data were analyzed using paired t-test and independent t-test in the significance level of (P<0.05). The results revealed that discontinuous training program significantly decrease the level of HCY, FIB, TNFα, IL-6, and CRP in obese women (P<0.05). Also the results of independent t-test indicated that there is a significant difference between discontinuous aerobic training and control groups regarding the considered variables (P<0.05).The current study suggested that the discontinuous aerobic training can lead to the plasma reduction on cardiovascular risk factors in obese women. This reduction can be influential in preventing, controlling, and decreasing cardiovascular biomarkers.