The aim of this study was to examine the effect of omega-3 supplementation on serum prostaglandin E2 in female athletes after a single bout of exhaustive exercsie. 22 female athletes (mean age 21.17±0.78 yr, body mass 21.24±2.32, VO2max 40.55±4.97) participated in study and divided into supplementation and placebo groups. Supplementation subjects daily received an omega-3 capsule containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and placebo subjects daily received a placebo capsule for 3 weeks. Blood samples were collected before and after the supplementation, before and after the exhaustive eercise. The prostaglandin E2 was measured by Eliza method. Data were analyzed by two-way analysis of variance at P<0.05. Results showed that prostaglandin E2 increased after the exhaustive exercsie (P<0.05). Omega-3 significantly decreased exercise-induced prostaglandin E2. The results of the present study suggested that consuming omega-3 can reduce inflammatory response to exhaustive exercise.