The aim of this study was to evaluate the effect of different intensities of short-term exercise on leptin, insulin, cortisol and lipid profiles in overweight and obese children. 24 overweight and obese boys (mean age 15.5±0.65 years old and BMI: 30.09+2.47 kg/m2) participated in this study. All subjects participated in a graded breath to breath gas-analyzer testing by Bruce protocol to measure VO2max. Then, based on the obtained VO2max, they were randomly divided into three groups of 50%, 75% and 85% intensity on a treadmill. Blood samples were collected before, immediately and 30 minutes after the exercise to evaluate plasma glucose, insulin, leptin, lipid profiles and cortisol. Results showed no significant differences among different intensities of short-term exercise in all variables. But intra-group changes showed the significant effect of exercise with 50% intensity on HDL (p=0.006), glucose (p<0.01) and leptin (p=0.004), exercise with 75% on TG (p=0.010), cholesterol (p=0.020) and glucose (p=0.006) and exercise with 85% intensity on HDL (p=0.001), cholesterol (p<0.01), glucose (p=0.038) and leptin (p=0.002). The results suggested that different intensities of a session of exercise had significant effects on leptin, insulin, cortisol and lipid profiles in overweight and obese adolescents, but there was no difference in the effect of these intensities.