The aim of this study was to investigate the effect of one session of continuous exercise on a treadmill on HSP72 and TAC levels of the hippocampus of male wistar rats. For this purpose, 20 male wistar rats (weight 165±1 gr. and age 6 to 8 weeks old) were assigned randomly into four groups: one control group and three acute endurance training groups (groups 1,2 and 3 were killed 30 minutes, 4 hours and 24 hours after the exercise respectively). Rats were subjected to treadmill exercise and the speed of the treadmill was 10m./min. and then gradually increased until 18 m./min. (grade 0%). The duration of the treadmill exercise was 60 minutes. The data were analyzed with one-way ANOVA and Tukey post hoc test (P?0.05). To determine HSP72 and TAC values sandwich Elisa and decolorization were used respectively. Following the acute continuous exercise, no significant change in HSP72 value in group 1(30 min.) and 2(4 hours) was observed compared to control group; but HSP72 value significantly increased in group 3 compared with control group (P=0.019) and group 1 (30 min.) (P=0.015). TAC level of hippocampus significantly increased in all exercise groups compared with control group (p=0.000). In general, acute exercise had a positive effect on HSP72 and TAC levels and increased their protective effect in the hippocampus of wistar rats.