The purpose of this study was to determine the effect of a session of endurance and sprint exercises on plasma ANP in male athlete students. For this reason, 24 male athlete students were volunteered and randomized into endurance (n=8, age 24.5± 2.1 years, height 176.3± 3.5 cm, weight 71.75±4.6 kg), sprint (n=8, age 22.1±2.2 years, height 176.1±4.8 cm, weight 67.6±7.4 kg) and control groups (n=8, age 23.6±2.6 years, height 174.7±7.9 cm, weight 71.6±7.9 kg). Endurance group ran 3200 m for 12 min and sprint group ran 100 m for 4 times with intervals, in which resting HR decreased to HR of exercise. 10 min pre- and post exercise were collected blood samples for measuring plasma ANP. Results of paired sample t-test showed : a session of endurance and sprint exercises increased significantly plasma ANP (p<0.05). Using of ANOVA test found no significant difference in pre-test plasma ANP (P>0.05) and a significant difference in post-test plasma ANP (P<0.05) in three groups. Inconclusion, a session of endurance and sprint exercises can effect on plasma ANP in male athlete students.