The aim of the present research was to study the effects of regular training and exhaustive exercise on t-PA/PAI-1 complex in the blood of forty eight rats. They were divided randomly into control and experimental groups and the subgroups included the primary acute, secondary acute and 8-wk regularly trained (each group 8 rats). Endurance training programs (five days a week, speed 15-22 m/min for 25-64 minutes) and exhaustive exercise (5 minutes of running with speed 20m/min, 10 minutes of running with speed 25m/min, and running with speed 30 m/min to exhaustion). Blood samples were gathered following 12 hours of overnight fasting. The tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1), t-PA/PAI-1 complex were measured by Elisa methods respectively. Data was analyzed by two-way ANOVA with repeated measures and independent t-student tests at P?0.05. The results showed that exhaustive exercise impaired fibrinolysis process and interestingly this destruction due exhaustive exercise after 8 weeks of activity was worse in training group than control group. Furthermore, significant increase in t-PA(P=0.04) and t-PA/PAI-1 complex (P=0.000) values and insignificant PAI-1(P=0.857) concentration was observed in the training group when compared with control group. In other words, regular training significantly increased t-PA (P=0.001) and insignificantly decreased PAI-1 (P=0.104) in training group when compared with the control group. These findings suggest that regular training does not highly influence destruction of hemostasis system due to exhaustive exercise. But regular training improves fibrinolysis process.