Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license
Authors
- mitra modirrousta 1
- khadijeh irandoust 1
- mohammad nabiuni 2
- naheed mojgani 3
- latifeh karimzadeh bardeei 4
1 Department of Sport Science, Faculty of Social Science, Imam Khomeini International University, Qazvin, Iran
2 Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
3 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization_Alborz, Iran.
4 Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Abstract
Introduction: Cholestasis is a chronic liver disease that is closely associated with an imbalance in the gut microbiota. Since exercise training is a potent mediator of lipolysis, it is an effective approach to prevent cholestasis-related damage. This study aimed to investigate the potential protective effects of exercise training and probiotic supplementation on cholestatic liver.
Method: 30 male Wistar rats (230-250 g) were randomly divided to five groups: control, cholestasis, cholestasis with exercise training (3times/week), cholestasis with probiotic treatment (5times/week , 108 CFU/ml), and cholestasis subjected to both exercise training and probiotic treatment for a duration of three weeks. Liver tissue was harvested for histomorphometric studies, TGFβ gene expression and blood serum was collected for analysis. Data were analyzed using one-way ANOVA and Tukey's post hoc test with a significance level of P<0.001.
Results: The results showed a significant decrease in the number of healthy hepatocytes and a significant increase in Kupffer cells, blood TBili and ALP levels, as well as an increase in TGFβ gene expression in the cholestasis group compared to the control (P<0.001). Exercise training and probiotic consumption alone or in synergic compensated for liver tissue damage and the number of hepatocytes, a decrease in the number of Kupffer cells and a decrease in the amount of ALP, TBili, and TGFβ gene expression in the treatment groups. (P<0.001)
Conclusion: it is possible that probiotics, combined with exercise or alone, could be used as a treatment protocol in patients with cholestasis by reducing the process of liver tissue damage.
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