Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license
Authors
1 Department of Exercise Physiology, Sar.C., Islamic Azad University, Sari, Iran.
2 Department of Cardiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Abstract
Introduction: Diabetic cardiomyopathy increases the risk of heart failure. Ischemic preconditioning (IPC) has a protective effect against myocardial injury. The present study aimed to investigate the effect of aerobic training and IPC with different muscle masses on myocardial NLRP3 and LC3 gene expression in diabetic wistar rats.
Methods: 35 wistar rats were randomly divided into seven groups: control (C), diabetic (D), one-leg ischemia (1IP), two-legs ischemia (2IP), aerobic training (T), one-leg ischemia+aerobic training (1IPT), two-legs ischemia+aerobic training (2IPT). Diabetes was induced by one-step injection of STZ (50 mg/kg). IPC was included three 5-minute cycles of ischemia, followed by five minutes of reperfusion. The aerobic training was performed for six weeks and included running on a treadmill. The speed for the first week is set at 9 meters per minute for 15 minutes. By the sixth week, the training speed is increased to 18 meters per minute for 30 minutes. NLRP3 and LC3 gene expression was measured by RT-PCR method. Data were analyzed by one-way ANOVA and Tukey post hoc tests.
Results NLRP3 expression significantly increased, and LC3 decreased in D compared to C (p=0.0001). NLRP3 and LC3 decreased and increased significantly, respectively, in 1IP (p=0.0001,p=0.021), 2IP (p=0.0001,p=0.003), T (p=0.0001, p=0.005), 1IPT (p=0.0001) and 2IPT (p=0.0001) compared to the C.
Conclusion: In conclusion, the effects of ischemic preconditioning on reducing cardiac NLRP3 and increasing LC3 are independent of the mass of ischemic muscle involved. Aerobic training with IPC is more effective in improving NLRP3 and LC3 in diabetes.
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