Document Type : Research Paper I Open Access I Released under CC BY-NC 4.0 license
Authors
1 Department of Exercise Physiology, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Corresponding Author, Department of Physical Education and Sport Sciences, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 Exercise Physiology Research Center, Lifestyle Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
4 Department of Physical Education and Sport Sciences, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract
Introduction: Autophagy is an anti-aging process and is very essential in heart health. The aim of the present study was to compare the effect of two models of high-intensity and moderate-intensity intermittent exercise on sirtuine genes and antioxidant defense factors in the heart muscle of elderly rats.
Methods: 24 male Wistar rats with an average weight of 350 ± 50 grams were randomly divided into control, moderate intensity continuous training (MICT) and high intensity interval training (HIIT) groups and underwent training for 8 weeks. Expression of Sirt1, Sirt3 genes was measured using real time-PCR technique and measurement of SOD and GPX concentration by optical absorption. To compare the groups, one-way analysis of variance was used at the alpha level of 0.05.
Results: The expression level of Sirt1 and Sirt3 genes in HIIT and MICT groups was significant compared to the control group (p<0.05). Also, there was no significant difference in Sirt1 between HIIT and MICT groups (p<0.05), and on the contrary, there was a significant difference in Sirt3 (p<0.05). SOD concentration in both HIIT and MICT groups was significant compared to the control group (p<0.05). GPX concentration in both HIIT and MICT groups was significant compared to the control group (p<0.05).
Conclusion: Results showed that both types of exercise moderated the expression of Sirt1 and Sirt3 genes compared to the non-exercise group. which probably activates the antiaging pathway of sirtuins in aging mice. Also, the concentration of antioxidant factors increased due to exercise, especially HIIT
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